Use of eugenol in Jundia da Amazonia (Leiarius marmoratus): effects on sedation and evaluation hemogasometry

This work aims to evaluate the use of eugenol as an anesthetic for jundia da Amazonia (Leiarius marmoratus), measuring the time to anesthesia induction in different concentracoes and their effects in gas exchange. The results were analyzed according to a completely randomized design (DIC) with eight treatments (control, 10, 20, 40, 80, 120, 150, 200 mg L-1 of eugenol) and ten repetitions. Time values of anesthetic induction and recovery, according to the level of eugenol underwent polynomial regression (p<0.05). The analysis of variance (ANOVA) and averages were compared by Tukey test. Were determined the time of anesthesia and recovery. The parameters were analyzed blood glucose, pH, partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), concentration of bicarbonate (HCO3-), sodium (Na), potassium (K), calcium (Ca) and chloride (Cl). The concentrations of 40 and 80 mg.L-1 of eugenol were the ones who presented time deep anesthesia induction of 59.5 +/- 17.5 sec. and 58.4 +/- 18.6 sec. respectively. The concentrations above 120 mg.L-1 presented mortality. These fish subjected to different level of eugenol did not show changes in blood oxygen pressure (PaO2) and carbon dioxide (PaCO2). The fish when subjected to concentrations above 80 mg L-1 showed an increase of bicarbonate. The plasma glucose values showed significant elevation in response to the bath with anesthetic eugenol. The concentrations of sodium, chloride, potassium and plasma protein remained constants indicating that the process of anesthesia was not long lasting enough to induce changes in the electrolyte balance. Within the conditions of this study, it can be concluded that eugenol is a suitable anesthetic for the silver. This product may be used at a concentration of 40 mg.L-1 without causing changes hemogasometrica.