4.0 Article

Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance

Journal

SEMINA-CIENCIAS AGRARIAS
Volume 33, Issue 2, Pages 687-695

Publisher

UNIV ESTADUAL LONDRINA
DOI: 10.5433/1679-0359.2012v33n2p687

Keywords

Essential oil; rosemary; bacterial resistance

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Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E. coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20 mu L oil pure rosemary were placed on the plates. After 24h/37 degrees C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria.

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