Article
Biochemistry & Molecular Biology
Justin Sui, Jennifer C. Boatz, Jian Shi, Qianjiang Hu, Xiaoyun Li, Yingze Zhang, Melanie Koenigshoff, Corrine R. Kliment
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease characterized by lung scarring and remodeling. This study investigates the role of adenine nucleotide translocases (ANTs) in IPF pathogenesis and finds that the loss of ANT1 leads to mitochondrial dysfunction, increased cellular senescence, and enhanced lung fibrosis. The findings suggest that modulation of ANTs could be a potential therapeutic avenue for limiting pulmonary fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Parasitology
Yihan Wu, Zhu Ying, Jing Liu, Zhepeng Sun, Shuang Li, Qun Liu
Summary: This study identified and characterized the ANT protein in T. gondii. Depletion of TgANT inhibited parasite growth, impaired mitochondrial and apicoplast biogenesis, and resulted in abnormal cell division.
PARASITES & VECTORS
(2022)
Article
Agronomy
Mei Deng, Li Long, Yukun Cheng, Fangjie Yao, Fangnian Guan, Yuqi Wang, Hao Li, Zhien Pu, Wei Li, Qiantao Jiang, Yuming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Jirui Wang, Youliang Zheng, Yunfeng Jiang, Guoyue Chen
Summary: In this study, three quantitative trait loci (QTL) associated with adult-plant stripe rust resistance were identified from the Chinese wheat landrace Yibinzhuermai. Among them, QYr.YBZR-6AL and QYr.YBZR-7DS had major effects in different field environments. Furthermore, haplotype analysis revealed that the resistance allele at QYr.YBZR-6AL was present in a small proportion of Chinese wheat landraces.
Article
Plant Sciences
Yawen Zhang, Xiaoping Xin, Cory Matthew, Michael J. Christensen, Zhibiao Nan
Summary: This study investigated a serious rust infection on grass species in the Hulunber grassland of Inner Mongolia, China. Two rust-causing pathogens were identified on Leymus chinensis, with Puccinia elymi being the major pathogen on this species and also infecting Agropyron cristatum and Festuca ovina. It was also found that Puccinia striiformis caused stripe rust on L. chinensis and Bromus inermis. The incidence and severity of rust infection increased throughout the growing season, especially on grass species closely related to common crop hosts of the pathogens. High host grass density and the presence of a potential alternate host were identified as factors promoting rust incidence. These findings provide important insights into the ecological factors associated with the rust epidemic and can contribute to the development of control strategies.
Article
Plant Sciences
Yunqian Wang, Cong Liu, Yuanyuan Du, Kunyan Cai, Yanfeng Wang, Jia Guo, Xingxuan Bai, Zhensheng Kang, Jun Guo
Summary: This study identifies a Pst effector, PstSIE1, that targets the immune component TaSGT1 and is involved in suppressing wheat defense. Overexpression of PstSIE1 increases the number of rust pustules and reduces the accumulation of reactive oxygen species (ROS).
Review
Cell Biology
Elena Frigo, Ludovica Tommasin, Giovanna Lippe, Michela Carraro, Paolo Bernardi
Summary: This article introduces the formation of Ca2+-activated, high-conductance channels by F1FO (F)-ATP synthase and adenine nucleotide translocase (ANT) in the inner membrane of mitochondria from various eukaryotes. The permeability transition (PT), a Ca2+-dependent increase in permeability in the inner mitochondrial membrane, and its molecular mechanisms have been challenging for scientists for the past 70 years. The features of the PT (or lack thereof) in mammals, yeast, fruit flies, brine shrimp, and nematodes, as well as the intrinsic pathway of apoptosis and other forms of cell death, are discussed in this review.
Article
Biochemistry & Molecular Biology
Camila Cimadamore-Werthein, Stephany Jaiquel Baron, Martin S. King, Roger Springett, Edmund R. S. Kunji
Summary: The mitochondrial ADP/ATP carrier plays a crucial role in oxidative phosphorylation by importing ADP into the mitochondrial matrix and exporting ATP. Recent studies have revealed that the carrier functions as a monomer with a single substrate binding site and operates with a ping-pong kinetic mechanism. These findings reconcile the discrepancy between the previous assumptions and provide insights into the alternating access mechanism of the carrier.
Article
Pharmacology & Pharmacy
Pengfei Zhang, Xinyu Cheng, Huimin Sun, Yajing Li, Wuxuan Mei, Changchun Zeng
Summary: ATR treatment can reduce the manifestation of fatty liver in HFD mice, decrease the accumulation of LDs in the liver, promote the activation of AMPK which leads to decreased mTOR activity, and facilitate autophagosome formation for accelerated lipid degradation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Jean Finlayson, Neusha Barakati, Paul R. Langlais, Janet Funk, Rocio Zapata Bustos, Dawn K. Coletta, Moulun Luo, Wayne T. Willis, Lawrence J. Mandarino
Summary: This study characterized ANT1 abundance and acetylation in human skeletal muscle using a novel method with small amounts of tissue samples. The findings indicate that acetylation of Lys23 reduces ADP binding to ANT1, providing insights into the potential effects of acetylation on ANT1 function in human muscle.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Cell & Tissue Engineering
X. Du, Z. Jiang, G. Fang, R. Liu, X. Wen, Y. Wu, S. Hu, Z. Zhang
Summary: This study aimed to investigate the role and mechanism of meniscal cell lysate (MCL) in fibroblast-like synoviocytes (FLSs) and osteoarthritis (OA). MCL was found to be substantially enriched in the synovial fluid of OA patients, promoting the release of inflammatory cytokines from FLSs through MCL phagocytosis. ANT3 was up-regulated in MCL and OA-FLSs, corresponding to impaired mitochondrial function and cell viability. Silencing ANT3 restored mitochondrial homeostasis, alleviating synovial inflammation and preventing early-stage OA.
BONE & JOINT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wayne Willis, Elizabeth Willis, Sarah Kuzmiak-Glancy, Katon Kras, Jamie Hudgens, Neusha Barakati, Jennifer Stern, Lawrence Mandarino
Summary: The study presents a luciferase-based assay for evaluating ADP kinetic parameters in mitochondria, showing that oxidative capacity and substrate combinations can dramatically influence ADP kinetics. The research also suggests that substrate-enhanced capacity to generate protonmotive force increases the OxPhos K(0.5)ADP, leading to flux control at ANT and subsequently affecting ADP-ANT binding dissociation constant.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Entomology
Zhen He, Yang Fang, Fengchao Zhang, Yang Liu, Xinkai Cheng, Jiajia Wang, Dechen Li, Dengsong Chen, Fan Wu
Summary: In Drosophila, Ant2 plays a crucial role in spermatid individualization and maturation. Knockdown of Ant2 leads to abnormalities in spermatid individualization and sperm maturation, resulting in male infertility. Ant2 knockdown also causes a decrease in tubulin polyglycylation, disruption of mitochondrial function, and an increase in apoptotic cells. Transcriptome and proteome analyses reveal significant changes in gene and protein expression, including reproductive-related glutathione transferases.
Article
Biochemistry & Molecular Biology
Pavla Pruchova, Klara Gotvaldova, Katarina Smolkova, Lukas Alan, Blanka Holendova, Jan Tauber, Alexander Galkin, Petr Jezek, Martin Jaburek
Summary: This study reveals the antioxidant and anti-inflammatory roles of iPLA(2)gamma in the brain. It demonstrates that iPLA(2)gamma is activated by tert-butylhydroperoxide and releases free fatty acids, which leads to an increase in respiratory rate and a decrease in mitochondrial superoxide production. It also shows that iPLA(2)gamma plays a key role in the remodeling of cardiolipins in the brain.
Article
Cell Biology
Fatih Yergoz, Julian Friebel, Nicolle Kraenkel, Ursula Rauch-Kroehnert, Heinz-Peter Schultheiss, Ulf Landmesser, Andrea Doerner
Summary: ANT1 in ischemic heart tissue promotes an anti-inflammatory immune response by increasing the infiltration of anti-inflammatory cells and reducing the activity of pro-inflammatory cells, resulting in improved cardiac function.
Article
Cell Biology
Corrine R. Kliment, Jennifer M. K. Nguyen, Mary Jane Kaltreider, YaWen Lu, Steven M. Claypool, Josiah E. Radder, Frank C. Sciurba, Yingze Zhang, Alyssa D. Gregory, Pablo A. Iglesias, Venkataramana K. Sidhaye, Douglas N. Robinson
Summary: Airway hydration and ciliary function are critical to airway homeostasis and dysregulated in chronic obstructive pulmonary disease (COPD), and members of the mitochondrial ADP/ATP transporter family adenine nucleotide translocase (ANT) play a protective role against cigarette smoke, highlighting a potential for upregulation of ANT proteins in COPD protection.
JOURNAL OF CELL SCIENCE
(2021)
Article
Plant Sciences
Yuanyuan Zhao, Xueling Huang, Qiao Li, Lili Huang, Zhensheng Kang, Jie Zhao
Summary: The emergence of new Puccinia striiformis f. sp. tritici races overcoming wheat resistance is a challenging issue. In a study conducted in Shaanxi Province, China, the phenotypic and genotypic diversity of the pathogen on barberry plants and its relationship with the population in wheat fields were determined. A total of 120 races and 132 genotypes were identified, indicating a close relationship and strong gene flow between the populations on barberry and wheat. This study highlights the important role of barberry plants in the epidemiology and management of stripe rust.
Article
Plant Sciences
Gensheng Zhang, Wei Liu, Lin Wang, Meng Ju, Xiaxia Tian, Zhimin Du, Zhensheng Kang, Jie Zhao
Summary: Through analyzing the avirulence and virulence of 120 selfed progeny lines from Berberis shensiana, we found that the TSA-6 Puccinia striiformis f. sp. tritici (Pst) isolate is virulent against the Yr5 resistance gene and exhibited 73 different virulence phenotypes compared to the parental isolate. Further analysis revealed the genetic composition of the TSA-6 isolate at various resistance loci and identified quantitative trait loci associated with virulence at 20 Yr resistance loci. These findings contribute to understanding Pst virulence evolution and simplifying breeding for wheat cultivars with effective resistance to wheat stripe rust.
Article
Plant Sciences
Zhimin Du, Yuelin Peng, Gensheng Zhang, Li Chen, Shuchang Jiang, Zhensheng Kang, Jie Zhao
Summary: This study revealed that the wheat stripe rust pathogen can undergo sexual reproduction on susceptible barberry in late autumn in Tibet, China. The findings contribute to the understanding of wheat stripe rust and provide insights into disease management in the region.
Article
Biochemistry & Molecular Biology
Jinping Wei, Xiaodong Wang, Zeyu Hu, Xiaojie Wang, Jialiu Wang, Jianfeng Wang, Xueling Huang, Zhensheng Kang, Chunlei Tang
Summary: The obligate biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) employs the effector Hasp98 to inhibit wheat's immune responses and promote the infection of P. striiformis. Hasp98 interacts with the wheat protein TaMAPK4, inhibiting its kinase activity and interfering with the MAPK signaling pathway. This study provides insights into how Pst effectors regulate host defense responses and promote stripe rust disease.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Linying Du, Xueling Huang, Li Ding, Zhongxue Wang, Dongling Tang, Bin Chen, Lanjiya Ao, Yuling Liu, Zhensheng Kang, Hude Mao
Summary: This study reveals the important role of TaERF87 in regulating drought tolerance in wheat. TaERF87 enhances drought tolerance by activating the expression of proline biosynthesis genes and synergistically interacts with TaAKS1. These findings contribute to the development of elite drought-tolerant wheat varieties.
Article
Biochemistry & Molecular Biology
Yingchao Sun, Xiaojie Wang, Feiyang Liu, Haoyu Guo, Jianfeng Wang, Zetong Wei, Zhensheng Kang, Chunlei Tang
Summary: Plant cell surface RLKs play a crucial role in recognizing and responding to pathogen invasion. In this study, a specific RLK gene (TaBIR1) was found to be upregulated during wheat resistance to Puccinia striiformis f. sp. tritici (Pst) infection. TaBIR1 was shown to be located on the plasma membrane and its knockdown resulted in decreased wheat resistance to stripe rust. Overexpression of TaBIR1 triggered constitutive immune responses in Nicotiana benthamiana. Moreover, TaBIR1's immune response is dependent on the homologues of NbBAK1 in wheat.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Fan Ji, Aihong Zhou, Bofan Liu, Yue Liu, Yaoxuan Feng, Xiaodong Wang, Lili Huang, Zhensheng Kang, Gangming Zhan
Summary: This study assessed the resistance risk of wheat leaf rust to the fungicide triadimefon in China. The majority of tested Pt isolates were sensitive to triadimefon, but 10.2% exhibited varying degrees of resistance. The resistant isolates showed strong adaptive traits in parasitic fitness. No correlation was found between triadimefon and other fungicides with similar or different modes of action. The risk of resistance to triadimefon in Pt may be low to moderate.
Article
Virology
Yanhui Zhang, Hualong Guo, Siyu Zhou, Daipeng Chen, Gang Xu, Zhensheng Kang, Li Zheng
Summary: We report a new mitovirus, named Puccinia striiformis mitovirus 2 (PsMV2), from the wheat stripe rust fungus. PsMV2 exhibited high multiplication during Pst infection and suppressed programmed cell death triggered by Bax. Silencing of PsMV2 reduced fungal growth and pathogenicity of Pst. Our results indicate that PsMV2 promotes pathogenicity and has a wide distribution in field isolates of Pst.
Review
Agronomy
Aqleem Abbas, Zhenhao Zhang, Hongxia Zheng, Mohammad Murtaza Alami, Abdulmajeed F. Alrefaei, Qamar Abbas, Syed Atif Hasan Naqvi, Muhammad Junaid Rao, Walid F. A. Mosa, Qamar Abbas, Azhar Hussin, Muhammad Zeeshan Hassan, Lei Zhou
Summary: Plant diseases pose a major threat to global food production. Traditional detection methods often fail during the early stages of pathogenesis, while remote sensing techniques using drones are effective for rapid identification of plant diseases. Drones play a crucial role in monitoring, detecting, and diagnosing plant pathogens, providing high-resolution data at a low cost.
Article
Biochemistry & Molecular Biology
Jia Guo, Ying Mou, Yuanxing Li, Qing Yang, Xue Wang, Haocheng Lin, Zhensheng Kang, Jun Guo
Summary: This study identified 131 chitinase genes in 10 fungal genomes and found 16 chitinase genes from Puccinia striiformis f. sp. tritici (Pst). The expression of PstChia1 was the highest during Pst infection and it showed chitinase enzyme activity. Silencing PstChia1 limited the growth and reduced the virulence of Pst, indicating its role in pathogenicity and interference with plant immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Muye Xiao, Dezhi Chen, Saifei Liu, Anle Chen, Anfei Fang, Binnian Tian, Yang Yu, Chaowei Bi, Zhensheng Kang, Yuheng Yang
Summary: A chitin deacetylase called PsCDA2 was identified in the wheat stripe rust fungus Pst, which inhibits plant cell death and protects the fungus from host chitinase. This discovery provides insights into the mechanism of plant immune suppression and has implications for wheat stripe rust control.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Wanlu Duan, Zhenkai Hao, Huihui Pang, Yuxi Peng, Yiwen Xu, Yanfei Zhang, Ying Zhang, Zhensheng Kang, Jing Zhao
Summary: Regulation of host gene expression by fungal pathogens is an important strategy for promoting disease. In this study, a fungal effector called PstGTA1 was identified from Puccinia striiformis f. sp. tritici (Pst). It was found to have transcriptional activating activity and contribute to the virulence of Pst by targeting the promoter of a wheat gene called TaSIG. This study provides the first evidence for a fungal effector modulating host gene transcription through promoter binding and histone acetylation.
Article
Plant Sciences
Aqleem Abbas, Amjad Ali, Azhar Hussain, Abdulwahed Fahad Alrefaei, Syed Atif Hasan Naqvi, Muhammad Junaid Rao, Iqra Mubeen, Tahir Farooq, Fatih Olmez, Faheem Shehzad Baloch
Summary: The study aimed to examine the genetic diversity and phylogenetic relationships of various R. solani anastomosis groups (AGs) associated with legume crops. It was found that different legume crops have distinct genetic differentiation within the same AGs, but isolates within the same AGs show high sequence similarity.
Article
Microbiology
Mengxin Zhao, Yanhui Zhang, Hualong Guo, Pengfei Gan, Mengmeng Cai, Zhensheng Kang, Yulin Cheng
Summary: This study identified and analyzed the function of CAP superfamily proteins in the wheat stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). The results showed that PsCAP1, PsCAP4, and PsCAP5 play significant roles in Pst infection in wheat.
Article
Microbiology
Chengcai Yan, Haiting Hao, Zhe Wang, Shuaishuai Sha, Yiwen Zhang, Qingpeng Wang, Zhensheng Kang, Lili Huang, Lan Wang, Hongzu Feng
Summary: Years of outbreaks of woody canker (Cryptosphaeria pullmanensis) in the United States, Iran, and China have resulted in massive economic losses. Utilizing the MaxEnt model, the spatiotemporal distribution of C. pullmanensis was predicted under twelve climate change scenarios. C. pullmanensis is mostly widespread in high-suitability regions, but suitable land areas are diminishing globally. Migration trend is towards higher latitudes and elevations, and China's estimated suitable area shifted eastward. The results of this study are valuable for countries where the infection has not yet fully spread and for nations where the species has been discovered.
