Article
Engineering, Biomedical
Jianhua Zhang, Hande Eyisoylu, Xiao-Hua Qin, Marina Rubert, Ralph Mueller
Summary: In this study, a novel human mesenchymal stem cell (hMSC)-laden GO/alginate/gelatin composite bioink was developed for forming 3D bone mimicking scaffolds. The GO composite bioinks showed improved bioprintability, scaffold fidelity, cell proliferation, osteogenic differentiation, and ECM mineralization. The optimal GO group was 1GO, which demonstrated great potential for 3D bioprinting of bone tissue models and tissue engineering applications.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Fei Huang, Hai Wang, Ying Zhang, Guozhen Wei, Yun Xie, Gui Wu
Summary: In this study, the inhibitor QNZ was investigated for its potential synergistic effect with BMP2 in promoting osteogenesis. It was found that QNZ can inhibit the proliferation and migration of fibroblasts, relieving their inhibitory effects on the differentiation of MSCs. The combination of QNZ and BMP2 showed enhanced osteoinductivity compared to BMP2 alone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Liudmila Leppik, Anna Gempp, Zyrafete Kuci, Selim Kuci, Peter Bader, Halvard Boenig, Ingo Marzi, Dirk Henrich
Summary: The clinical breakthrough of bone tissue engineering relies on providing consistent pharmacological quality BTE products, which is hindered by the availability of stem cells. To overcome this, the suggestion is to immobilize heterologous osteoinductive MSCs onto osteoconductive matrices, freeze them, and then release them as ready-to-use products for surgery. In our study, we found that freeze-thawed hMSCs maintained their metabolic activity and showed favorable osteogenic potential, possibly through the activation of HIF1 alpha and YBX1 genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.
Article
Engineering, Biomedical
Milica Marinkovic, Rukmani Sridharan, Francesco Santarella, Avi Smith, Jonathan A. Garlick, Cathal J. Kearney
Summary: Extracellular matrix, particularly collagen and glycosaminoglycan, is crucial for wound healing. Fibroblasts derived from iPS cells provide a novel source for scaffold fabrication to improve wound healing. Optimization of matrix production using macromolecular crowding, specifically Ficoll, significantly enhances collagen deposition without affecting composition, while Carrageenan increases glycosaminoglycan deposition. This work lays the groundwork for developing a cost-effective protocol for iPSF ECM production for tissue engineering scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Review
Respiratory System
Sakshi Phogat, Fama Thiam, Safiya Al Yazeedi, Filsan Ahmed Abokor, Emmanuel Twumasi Osei
Summary: The pulmonary extracellular matrix (ECM) plays a vital role in lung development, repair, and maintenance of tissue homeostasis. It regulates the function of lung fibroblasts, which are responsible for the production and repair of ECM proteins. In lung injury and chronic lung diseases, an abnormal feedback between fibroblasts and the altered ECM disrupts tissue homeostasis and leads to fibrotic changes and tissue remodeling.
RESPIRATORY RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
F. Urciuolo, G. Imparato, P. A. Netti
Summary: The extracellular microenvironment plays a crucial role in regulating cell functions and tissue development. Misregulation of this cell-ECM interaction can lead to dysfunctional tissues and diseases. Tissue engineering approaches should aim to recapitulate the native cell microenvironment to achieve functional tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Oncology
Kavitha Unnikrishnan, Lynda Velutheril Thomas, Ram Mohan Ram Kumar
Summary: The lack of traditional cancer treatments has led to a demand for new clinical techniques. Utilizing 3D culture systems allows for the recreation of in vivo tumor microenvironments, leading to better modeling of human tumors and improved therapy results. The use of 3D scaffold models in cancer tissue engineering has the potential to enhance preclinical studies and facilitate clinical translation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Engineering, Biomedical
Changru Zhang, Ya Ren, Weiqing Kong, Yihao Liu, Heyue Li, Han Yang, Bin Cai, Kerong Dai, Chengwei Wang, Liang Tang, Haoyi Niu, Jinwu Wang
Summary: In this study, a double-bond-functionalized photocurable mesoporous bioglass sol was developed for the fabrication of personalized and highly porous PMBG/TCP biphasic scaffolds. The scaffolds exhibited regulated mechanical properties and degradation behavior through the varying amount of TCP doping. In vitro and in vivo experiments confirmed the release of SiO2 and Ca2+ from the scaffolds, creating a bone regeneration microenvironment that promotes angiogenesis and accelerates bone tissue regeneration.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Biotechnology & Applied Microbiology
Maria Grazia Tupone, Michele d'Angelo, Vanessa Castelli, Mariano Catanesi, Elisabetta Benedetti, Annamaria Cimini
Summary: Exploring and developing multifunctional intelligent biomaterials is crucial for improving next-generation therapies in tissue engineering and regenerative medicine. These biomaterials can mimic the in situ microenvironment and provide specialized bioactive signals to regulate the surrounding biological habitat, contributing to wound healing and biomaterial integration. The connection between stem cells and biomaterials can stimulate specific modifications in cell properties and fate, influencing processes such as self-renewal, adhesion, and differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Environmental
Rezgar Hasanzadeh, Peyman Mihankhah, Taher Azdast, Asghar Rasouli, Mohadese Shamkhali, Chul B. Park
Summary: This article introduces the advantages and applications of 3D and 4D printing in fabricating tissue engineering scaffolds, with a focus on the potential of hydrogels in 4D printing scaffolds. Furthermore, the effects of 3D and 4D printing parameters on the mechanical, morphological, and biological properties of scaffolds are investigated.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Antonia Vogt, Konstantinos Kapetanos, Neophytos Christodoulou, Dimitrios Asimakopoulos, Mark A. Birch, Andrew W. McCaskie, Wasim Khan
Summary: Tissue engineering and cell therapy have the potential to treat chronic disorders. This systematic review aims to assess the influence of patient age on the chondrogenic potential of mesenchymal stromal cells (MSCs) in regenerative therapy. The review reveals conflicting outcomes regarding the chondrogenic potential of MSCs in relation to age.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Binbin Sun, Meifei Lian, Yu Han, Xiumei Mo, Wenbo Jiang, Zhiguang Qiao, Kerong Dai
Summary: An anatomically correct IVD scaffold was fabricated using 3D bioprinting technology, which successfully regenerated the structure and function of the nucleus pulposus and annular fibrosus in vivo. The scaffold exhibited good biomechanical function and demonstrated the clinical application potential of dual growth factors-releasing IVD scaffold. Evaluation in large mammal animal models is necessary for further study.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Mai Yoshino, Mikihito Kajiya, Hiroki Yoshii, Shin Morimoto, Susumu Horikoshi, Misako Tari, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: This study compares the biological properties of widely-used MSC spheroids and newly-developed C-MSCs, providing insights into the distinct features of each 3D cell culture method and their potential applications in MSC-based cell therapy.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Yilin Han, Marianne King, Evgenii Tikhomirov, Povilas Barasa, Cleide Dos Santos Souza, Jonas Lindh, Daiva Baltriukiene, Laura Ferraiuolo, Mimoun Azzouz, Maurizio R. Gullo, Elena N. Kozlova
Summary: The study aims to determine the survival and differentiation of boundary cap neural crest stem cells (BC) in gelatin-based 3D bioprinted bioink scaffolds for the fabrication of spinal cord organoids on a chip. The gelatin-based and enzymatically cross-linked hydrogel was found to be a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but further measures are needed to achieve uniform neural differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Shani Attias Cohen, Haneen Simaan-Yameen, Claudia Fuoco, Cesare Gargioli, Dror Seliktar
Summary: This study presents a hydrogel-based strategy for encapsulating therapeutic AONs, which can enhance stability and cellular uptake. The injectable PF microspheres can sustainably release the AONs and achieve cellular uptake, making it a promising approach for treating DMD.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Yingchun Su, Christoph Alexander Mueller, Xuya Xiong, Mingdong Dong, Menglin Chen
Summary: This article proposes two novel strategies for functionalizing melt electrowritten microfibrous scaffolds with nanomaterials to enhance osteogenic differentiation for tissue regeneration. The modified scaffolds showed significantly higher calcium mineralization levels and superior results in terms of ALP activity and mineralization compared to the nonmodified scaffolds. The nanotopography mimicking bone anatomy contributed to the improved performance.
Article
Engineering, Biomedical
Raziel Hamami, Haneen Simaan-Yameen, Cesare Gargioli, Dror Seliktar
Summary: This study compares four different bioprocessing techniques for producing hydrogel microcarriers, aiming to achieve effective cell encapsulation while maintaining high cell viability and small particle size for injectability. The results show that the reverse thermal gelation technique produces cell-laden microgels with minimal specialized equipment and bioprocessing steps. The average microgel size is significantly smaller and more uniform when produced without cells.
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Sofie Husted Laursen, Signe Gellert Hansen, Mehmet Berat Taskin, Menglin Chen, Lise Wogensen, Jens Vinge Nygaard, Susanne Maigaard Axelsen
Summary: This study provides new insights into future pelvic organ prolapse (POP) repair in postmenopausal women by demonstrating the sufficient support and biocompatibility of electrospun polycaprolactone (PCL) meshes coated with stem cells and growth factor in elderly rats in the long term, thus highlighting its potential clinical significance.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Engineering, Environmental
Yanping Zhang, Alice Le Friec, Di Sun, Menglin Chen
Summary: The development of flexible electronics with adjustable performance offers a promising approach for treating cardiac diseases, overcoming the limitations of conventional electronic systems due to biomechanical mismatches. By exploring the natural flow behavior of polymer melt in Melt electrowriting (MEW), researchers created a fibrous mesh electrode with a sinusoidal anisotropic structure resembling the collagen fiber architecture in the heart. This anisotropic, stretchable electrode mesh demonstrated the ability to synchronize cardiomyocyte beating through its biomimetic viscoelastic behavior and fine-tuned electrical properties, offering great potential for cardiac repair.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
C. A. Mueller, P. Li, Y. Wang, M. Dong, B. Tian, M. Chen
Summary: Mounting evidence supports the central role of the nervous system in disease pathogenesis. Wireless electrical modulation of neural cells via opto-stimulation is an emerging approach to control neural behavior. This study successfully combines bionic fibers with nano-groove topography and opto-response to guide neurite outgrowth, providing a potential tool for studying nervous system/disease interaction.
MATERIALS TODAY NANO
(2023)
Article
Engineering, Biomedical
Jordi Amagat, Yingchun Su, Frederik Hobjerg Svejso, Alice Le Friec, Steffan Moller Sonderskov, Mingdong Dong, Ying Fang, Menglin Chen
Summary: In this study, porous and conductive hydrogel-based nerve guidance conduits (NGCs) with anisotropic, self-snapping properties were successfully fabricated for peripheral nerve regeneration. The electroactive g-C3N4 H/rGO3 hydrogel supported normal viability and proliferation of neural cells, with significantly longer neurites observed compared to pristine g-C3N4 H hydrogel. Furthermore, the NGC with aligned microchannels showed optimal neurite guidance, indicating great potential for repairing peripheral nerve injuries.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Physical
Zhiqun Xie, Dionysios D. Dionysiou, Shuang Luo, Menglin Chen, Zongsu Wei
Summary: In order to enhance the utilization of peroxymonosulfate (PMS) and the stability of catalyst, a dual-reaction center catalyst Cu-Mg0.388Al2.408O4-BN was developed without expensive rare metals. The presence of Mg resulted in a positively charged surface, which facilitated the adsorption of bisphenol A (BPA) and PMS anions under neutral pH conditions. The formation of an electron-rich Cu center was achieved due to the relatively low electronegativity of Mg and the cation-pi interaction between Cu and boron nitride (BN). The combination of electrostatic attraction and dual-reaction center enabled the rapid degradation of BPA through electron transfer and subsequent activation of PMS.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2023)
Article
Cell Biology
Alessio Reggio, Francesca De Paolis, Salma Bousselmi, Felice Cicciarelli, Sergio Bernardini, Alberto Rainee, Dror Seliktar, Stefano Testa, Carmine Cirillo, Paolo Grumati, Stefano Cannata, Claudia Fuoco, Cesare Gargioli
Summary: In human dystrophies, ectopic deposition of fat and fibrous tissue from FAPs exacerbates progressive muscle wasting. LY2090314 interferes with adipogenesis and has the potential to limit FAP-derived fat infiltrates in dystrophic muscles.
DISEASE MODELS & MECHANISMS
(2023)
Article
Chemistry, Applied
Farzaneh Nasrollahzadeh, Maria Julia Spotti, Kasper Skov, Tizazu Mekonen, Menglin Chen, Mario M. Martinez
Summary: The study investigates the impact of incorporating polar gelatin and non-polar zein on the mechanical properties of high moisture meat analogue prototypes. The addition of these proteins alters the elasticity and tenderness of the extrudates, with different effects observed for soy and mung bean prototypes. The concentration-dependent changes in protein structures and water mobility highlight the potential of multifunctional fillers and top-down/bottom-up approaches for creating plant-based structured foods with meat-like texture.
FOOD HYDROCOLLOIDS
(2023)
Article
Engineering, Biomedical
Revital Goldshmid, Haneen Simaan-Yameen, Liaura Ifergan, Claudia Loebel, Jason A. Burdick, Dror Seliktar
Summary: This study evaluates how mechanical properties of a scaffold can alter the phenotype and genotype of human mesenchymal stromal cells (hMSCs), aiming to enhance their differentiation potential along the myogenic, neurogenic, or chondrogenic lineages. The shear storage modulus of a hydrogel scaffold was controlled by adjusting the amount of cross-linker, and it was found that materials with lower storage moduli promoted neurogenic differentiation, while those with intermediate moduli favored myogenic differentiation, and higher moduli favored chondrogenic differentiation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Biomaterials
Jordi Amagat, Christoph Alexander Mueller, Bjarke Norrehvedde Jensen, Xuya Xiong, Yingchun Su, Natasja Porskjaer Christensen, Alice Le Friec, Mingdong Dong, Ying Fang, Menglin Chen
Summary: We developed an injectable, nanofibrous 2D flexible hydrogel sheet-based neural stimulation device that can be non-invasively implanted via syringe injection for optoelectrical and biochemical dual stimulation of neurons. The device consists of mechanically reinforced nanofibers loaded with photocatatlytic g-C3N4/GO nanoparticles for blue light stimulation and nerve growth factor (NGF) loaded nanofibers for biochemical stimulation of neural differentiation. This device represents a crucial early step in developing a minimally invasive system for neural stimulation.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Electrical & Electronic
Bjarke Norrehvedde Jensen, Yuting Wang, Alice Le Friec, Sadegh Nabavi, Mingdong Dong, Dror Seliktar, Menglin Chen
Summary: A wireless electromagnetic neural stimulation patch has been developed, which combines electromagnetic induction and physical guidance cues to promote neural cell growth and treat neurological diseases. In vitro experiments confirmed the biocompatibility and efficacy of the patch's electromagnetic stimulation in promoting neurite outgrowth, demonstrating its great potential for non-invasive neurological therapeutics.
NPJ FLEXIBLE ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Ruodan Xu, Junwei Gao, Can Cao, Mingfei Shi, Yonghui Zhang, Shihao Hong, Shijie Guo, Menglin Chen, Ping Song, Gaoshuang Fu, Jing Li, Tengxiao Liang, Yingchun Miao, Lu Tang, Jinsheng Yang, Ning Li, Mingdong Dong
Summary: This study investigates the susceptibility of Alzheimer's disease (AD) patients with Apolipoprotein (APOE) e4 to COVID-19 and the underlying mechanism. The findings demonstrate that AD patients have increased invasion of SARS-CoV-2 in their lung cells, along with altered expression of viral receptors and co-receptors. Additionally, the study shows that HDL mimetics can protect APOE e4-bearing lung cells against SARS-CoV-2 invasion, potentially offering a therapeutic strategy for COVID-19.
