Journal
SCIENTIA HORTICULTURAE
Volume 161, Issue -, Pages 170-180Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2013.06.014
Keywords
Dendrobium 'Zahra FR 62'; Plant growth regulators; Protocorm-like body; In vitro propagation; Culture system
Categories
Funding
- Indonesian Ornamental Crops Research Institute
- Indonesian Agriculture Research and Development Agency
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A mass propagation protocol for Dendrobium 'Zahra FR 62', the result of hybridization between Dendrobium 'Sonia Deep Pink' x Dendrobium 1265, was successfully established. Half-strength Murashige and Skoog (MS) medium containing 1 mg/L thidiazuron (TDZ) and 0.5 mg/L N-6-benzyladenine (BA) resulted in high protoccorm-like body (PLB) formation and initial proliferation of Dendrobium using shoots as the donor explant via repeated subcultures. High proliferation and optimal growth of PLBs was possible by culturing PLBs in a 3-L bioreactor with 10 vessel volumes per minute (vvm) (CS-9) containing half-strength MS medium with 0.05 mg/L BA (M-2). A total of 807.7 PLBs formed with an average of 247.5 new PLBs per subculture. The culture system was improved by adding 15 g of explant to 5 vvm of dissolved oxygen, although good quality PLBs formed when 10 g of explant was combined with 10 vvm of dissolved oxygen. PLBs converted into healthy plantlets by culturing them on half-strength MS containing 0.05 mg/L BA. Thereafter, germinated PLBs were subcultured on half-strength MS medium with full vitamins and free of plant growth regulators (RM-1). Rooted plantlets were successfully acclimatized ex vitro with 90-100% survival using Cycas rumphii bulk and grew well after repotting individually into a mixture of C. rumphii bulk and wood charcoal (1:1, v/v). (C) 2013 Elsevier B.V. All rights reserved.
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