Journal
SCIENTIA HORTICULTURAE
Volume 158, Issue -, Pages 16-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2013.04.019
Keywords
Cut rose flower; Reference gene; RT-PCR; qRT-PCR
Categories
Funding
- National Natural Science Foundation of China [31171992]
- Beijing Nova Program [2009B51]
Ask authors/readers for more resources
Optimal reference genes are important for data normalization so that accurate and reliable gene expression measurements may be obtained in both semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR (qRT-PCR) methods. This study firstly evaluated potential reference genes in petals of rose flower (Rosa hybrida) under postharvest stress conditions and in various floral organs during flower opening and senescence, combining both RT-PCR and qRT-PCR analysis. The expression stabilities of gene members from three traditional housekeeping gene families - actin (RhACT), tubulin (RhTUB) and ubiquitin (RhUBI) - were assessed using two analysis software packages, geNorm and NormFinder. The results showed that, for cut rose flower, the optimal reference genes were RhUBI1 for dehydration treatment and receptacles; RhTUB2 for exogenous ethylene; RhACT4 for gibberellic and abscisic acid treatments, wounding and stamens; RhUBI6 for petals; RhUBI2 for sepals; and RhACT1 for gynoecia, respectively. Our results provide guidelines for reference gene(s) selection under different postharvest conditions and point the way towards more accurate and widespread use of qRT-PCR in rose flower. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available