Journal
SCIENTIA HORTICULTURAE
Volume 129, Issue 3, Pages 449-458Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2011.04.015
Keywords
CHS; Promoter; Malus crabapple; cis-Element; Transgenic tobacco
Categories
Funding
- National Natural Science Foundation of China [31071785]
- Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality [PHR20110515]
- Fruit Tree Key Laboratory of Beijing University of Agriculture (BUA)
- Beijing Key Laboratory for Agricultural Application and New Technique
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Anthocyanins are secondary metabolites of higher plants that contribute to the colors of leaves and flowers, and chalcone synthase is the first key enzyme in anthocyanin biosynthesis. We isolated a 2.4-kb and a 1.8-kb 5'-upstream sequence of chalcone synthase (McCHS) from 'ever-green-leaf and 'ever-red-leaf color types of Malus crabapple cvs. 'Flame' and 'Royalty', respectively, and found a 743-bp fragment missing from 'Flame' when comparing these sequences. The missing sequence contained two MYBPLANT elements and three MYBCORE elements that are essential for MYB transcription-factor binding and inducing anthocyanin biosynthesis. Thus, we concluded that the lack of these important cis-elements was a reason for the low expression level of McCHS in 'Flame'. GUS activity and transient expression assays showed a series of 12 deletion promoter fragments had promoter activity and could drive GUS expression: however, the GUS staining color and the GUS activity were different, indicating that different fragments had different promoter activity. Tissue expression assays in stable transgenic tobacco showed that the McCHS promoter was expressed widely, i.e. in leaves, roots and stems. To sum up, the McCHS promoter has potential application in genetic transformation to improve plant color. (C) 2011 Elsevier B.V. All rights reserved.
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