Article
Biochemistry & Molecular Biology
Jorge Sanchez-Lopez, Alejandro Atares, Marybel Jaquez-Gutierrez, Ana Ortiz-Atienza, Carmen Capel, Benito Pineda, Begona Garcia-Sogo, Fernando J. Yuste-Lisbona, Rafael Lozano, Vicente Moreno
Summary: Screening of 862 T-DNA lines identified mutants defective in different phases of indirect adventitious organogenesis in tomato, including callus growth, bud differentiation, and shoot-bud development. Mutants of TDC-1 and TDS-1 genes showed specific roles in cell proliferation and organization of adventitious shoot meristems, respectively, while mutants of TDB-1, -2, -3 genes exhibited multiple changes in vegetative and reproductive traits, with the discovery of the involvement of the SlMAPKKK17 gene in shoot-bud differentiation.
Article
Biotechnology & Applied Microbiology
H. David Raja, P. Srinivasan, R. Tamilvanan
Summary: This study aims to produce genetically pure clones of N. alata through indirect organogenesis using purified Vermicast Extract and various concentrations of plant growth regulators. An efficient protocol for indirect organogenesis of Naregamia alata using purified VCE was established. The combination of purified VCE and plant growth regulators was found to enhance the regeneration ability and genetic stability of N. alata.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Manal El-salato Ala El-naby Ahmed
Summary: A successful and efficient micropropagation protocol has been developed for the clonal propagation and conservation of Dimocarpus longan. The use of salicylic acid and methyl jasmonate as elicitors for increasing in vitro production of secondary metabolites in plants is demonstrated in this work.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Pooja Jaiswal, Nishi Kumari, Sarvesh Pratap Kashyap, Shailesh Kumar Tiwari
Summary: The in vitro raised plantlets were obtained through direct organogenesis and served as donor plants for the collection of leaf explants. Successful induction of callogenesis and shoot differentiation was observed through the manipulation of cytokinins in the culture medium. The genetic fidelity of micropropagated plants was confirmed through molecular analysis using SCoT and ISSR markers, with 12.5% polymorphism reported due to callus-mediated shoot regeneration.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Zishan Ahmad, Vikas Yadav, Anwar Shahzad, Abolghassem Emamverdian, Muthusamy Ramakrishnan, Yulong Ding
Summary: Casuarina equisetifolia, an important tree in tropical and subtropical regions, has been successfully propagated in vitro using nodal segments. This micropropagation protocol can be used for large-scale multiplication and germplasm preservation, meeting the increasing demands of C. equisetifolia in the forest industry.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Thirupathi Koppula, Dulam Sandhya, Gulab Khan Rohela, Saritha Kommidi, Mustafa Mohammed
Summary: Solena amplexicaulis is a creeping cucurbit with high medicinal values. In this study, in vitro micropropagation was successfully used as an alternative method for its propagation and conservation. By optimizing the plant growth regulators and culture conditions, a large number of shoots were induced and subsequently rooted. The resulting plantlets showed high survival rates when transferred to natural field conditions. The genetic fidelity of tissue-cultured plants was also confirmed.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Plant Sciences
Biswajit Pramanik, Sutanu Sarkar, Somnath Bhattacharyya, Saikat Gantait
Summary: The present study focused on optimizing the in vitro regeneration system of Bacopa monnieri, achieving advancements in proliferation, synthetic seed production, acclimatization, and genetic fidelity assessment. The optimal medium composition and hormone concentrations promoted shoot multiplication and rooting from shoot tip explants. Synthetic seeds produced using sodium alginate and calcium chloride successfully regenerated in half-strength MS medium.
ACTA PHYSIOLOGIAE PLANTARUM
(2021)
Article
Plant Sciences
Jung-Woo Lee, Jang-Uk Kim, Kyong-Hwan Bang, Nayeong Kwon, Young-Chang Kim, Ick-Hyun Jo, Young-Doo Park
Summary: This study aims to establish an efficient regeneration and acclimatization system for Panax ginseng Meyer, a crop with high demand due to its medicinal properties but hindered by low reproductive efficiency. The results showed that the basal media MS, N6, and GD with specific nitrogen content and NH4+/NO3- ratio yielded the highest rate of somatic embryogenesis. Full-strength MS medium was best for somatic embryo induction, while diluted MS medium had a more positive effect on embryo maturation. The germination medium containing 1/2 MS facilitated good shoot development and 1/2 SH medium yielded outstanding root development.
Article
Biotechnology & Applied Microbiology
Jyoti Mamgain, A. Mujib, Bushra Ejaz, Basit Gulzar, Moien Qadir Malik, Rukaya Syeed
Summary: An efficient regeneration method has been developed in Tylophora indica, with histology and molecular markers indicating genetic homogeneity of the regenerated population. This study is important for addressing the challenges of large scale propagation of the plant in the wild.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Chemistry, Physical
M. Manokari, M. Cokul Raj, Abhijit Dey, Mohammad Faisal, Abdulrahman A. Alatar, Nirmal Joshee, Mahipal S. Shekhawat
Summary: This study investigated the impact of Silicon nanoparticles (SiNPs) on in vitro adventitious root formation and structural developments in Hedyotis biflora. It was found that SiNPs promoted the morpho-anatomical improvements in roots, resulting in thicker, sturdier roots with increased biomass and length. Microscopic analysis showed that SiNPs-derived roots had fully differentiated structures, while control roots only had primary structures. The addition of SiNPs also improved acclimatization efficiency and survival rate of the plantlets.
Article
Agronomy
Piotr Palka, Bozena Muszynska, Agnieszka Szewczyk, Bozena Pawlowska
Summary: In this study, the phenolic compound content and composition of Lilium candidum adventitious bulbs formed in vitro were determined. The use of zinc oxide nanoparticles (ZnO NPs) in the culture medium, along with LED light spectra dominated by red and blue light, significantly increased the content of phenolic compounds.
Article
Biotechnology & Applied Microbiology
Genhai Hu, Maoni Chao, Yuanzhi Fu, Xiaohong Zhang
Summary: A protocol for bud induction from adventitious roots of purple sweet potato and a new method for acclimatizing regenerated seedlings by hydroponics were successfully developed in this study. The use of purple sweet potato leaves as explants, along with different hormones at different stages, resulted in the induction of callus, adventitious roots, and buds. A highly efficient plant regeneration system in sweet potato was established, which may be valuable for transgenic genetic improvement.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Plant Sciences
Kourosh Vahdati, Rasoul Sadeghi-Majd, Adriana F. Sestras, Ricardo Julian Licea-Moreno, Augusto Peixe, Radu E. Sestras
Summary: Walnut propagation methods are crucial for maintaining genetic structure and transmitting traits to offspring. Grafting, cutting, and in vitro culture are commonly used techniques, but are influenced by various factors. In vitro culture is the most suitable method for walnut cloning, but it has limitations for commercial propagation.
Article
Plant Sciences
Abdul Kader, Sankar Narayan Sinha, Parthadeb Ghosh
Summary: This study successfully produced genetically stable plants from callus of Ficus religiosa using shoot tip explants. Different morphological calluses were formed based on plant growth regulator treatments, and the highest organogenesis response and shoot length were achieved with specific combinations of growth regulators. The regenerated microshoots were further multiplied and hardened under different conditions. The study also demonstrated the absence of any variation among the callus-derived plantlets with their respective mother donor plant.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Plant Sciences
Dilmini Alahakoon, Anne Fennell
Summary: This study analyzed the genetic basis of grapevine root system architecture, which is important for improving sustainability in grapevine cultivation under changing climates. The researchers identified 31 QTL associated with various root traits and identified candidate genes that could be used for breeding programs to tailor grapevine root system characteristics.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Peng Jia, Rui Yan, Yuan Wang, Feng-huan Gao, Yang Liu, Qing-long Dong, Hao-an Luan, Xue-mei Zhang, Han Li, Su-ping Guo, Guo-hui Qi
Summary: The KNOX gene plays crucial roles in plant development, and this study identified 19 KNOX gene members in kiwifruit. One of the genes, AcKNOX11, is selectively expressed in flower buds and shoots, and its expression can be induced by ABA and melatonin. Functional analysis revealed that AcKNOX11 delays flowering, reduces plant height, alters leaf shape, and inhibits seed germination through ABA-melatonin interaction.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Dan Hua, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Huan-Huan Chen, Ning-Wei Lai, Lin-Tong Yang, Xin Ye, Li -Song Chen
Summary: This study revealed that nitrogen deficiency can lead to increased production of reactive oxygen species (ROS) and methylglyoxal (MG), but also enhances the ability of leaves and roots to detoxify these molecules. The effects of nitrogen deficiency on ROS and MG generation and their detoxification systems were found to be different in leaves and roots, with roots being more affected.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Shenghan Yang, Chonlong Chio, Wensheng Qin, Yanxi Pei, Guangpeng Pei, Yuen Zhu, Hua Li
Summary: This study evaluates the immobilization and deactivation of pathogen-produced enzymes and toxic metabolites by biochar in tomato Fusarium wilt and finds that biochar can effectively prevent disease occurrence.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Deborah Pagliaccia, Michelle Ortiz, Michael Rodriguez, Sophia Abbott, Agustina De Francesco, Madison Amador, Valeria Lavagi, Benjamin Maki, Francesca Hopkins, Jonathan Kaplan, Samantha Ying, Georgios Vidalakis
Summary: This study investigates the effectiveness of organic soil amendments (bokashi, biochar, and their combination) in promoting soil health, nutrient availability, and plant growth. The results show that these amendments can significantly alter soil parameters and have positive effects on soil and plant health.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yu-Jeong Kwon, Min Jeong Hong, Minkyu Park, Dawon Jeon, Gah-Hyun Lim, Sungyul Chang, Dong-Hwan Kim, Jin-Baek Kim, Jundae Lee, Yeong Deuk Jo
Summary: Flowing LOCUS T (FT)-like genes play crucial roles in flowering induction in plants. Pepper FT-like genes are mostly expressed in leaves and interact with FLOWERING LOCUS D, promoting or delaying flowering. The unique evolutionary process of pepper FT-like genes provides insights for improving productivity.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Ziwen Su, Juan Yan, Binbin Zhang, Meng Sun, Zhixiang Cai, Zhijun Shen, Ruijuan Ma, Mingliang Yu
Summary: Bagging is a crucial cultivation measure that affects the quality of peach fruit by regulating exposure to light. This study investigated the effects of bagging on the content of phenolic compounds in peach fruit and identified the most sensitive compounds to bagging treatment. The study also explored the regulatory role of bagging on phenolic synthetic-related genes. The results showed that bagging affected the accumulation of different phenolics in different peach cultivars and light intensity played a significant role in the accumulation of anthocyanins and other phenolic substances in peach fruit. Additionally, the expression of structural genes and transcription factors related to phenolic synthesis was regulated by bagging and light conditions. These findings provide a foundation for understanding the regulation mechanism of light on the synthesis of phenolic compounds in peach fruit.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yanfang Zhang, Xinzhi Cui, Zhilei Du, Xiulian Li, Bangdi Liu, Meng Liu, Xiangquan Zeng, Fengjun Guo, Xinguang Fan, Shuyang Sun
Summary: In this study, melatonin treatment effectively improved the aroma quality of apricots during low temperature storage by maintaining firmness and soluble solids content, inhibiting ethylene production, and regulating enzyme activities related to aroma synthesis.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Sang-Mo Kang, Shifa Shaffique, Md. Injamum-Ul-Hoque, Sarah Owdah Alomrani, Yong-Sung Park, In-Jung Lee
Summary: Salinity is a global environmental problem that affects the physiology and morphology of plants. Melatonin has been found to improve photosynthesis, antioxidant activities, and seedling characteristics in milk thistle plants exposed to salinity stress, thereby enhancing their salt tolerance.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Nadia Borgini, Haifa Benmoussa, Mohamed Ghrab, Mehdi Ben Mimoun
Summary: This study investigates the agroclimatic requirements of Prunus species cultivars growing in warm areas using Partial Least Squares regression. The findings reveal that the chilling and heat requirements of the cultivars appear discontinuous, with overlaps or transition periods between the two phases. The warm mean temperatures occurring during the chilling period are the main determinant of the flowering of the studied cultivars.
SCIENTIA HORTICULTURAE
(2024)
Review
Horticulture
Kayise Hypercia Maseko, Thierry Regnier, Belinda Meiring, Obiro Cuthbert Wokadala, Tonna Ashim Anyasi
Summary: Bananas are a significant tropical fruit with diverse cultivars, providing essential minerals, vitamins, and phytochemicals. The Cavendish group is preferred, but identifying suitable cultivars and maturity stages is crucial for various applications.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Godfrey Ouma, Joshua Wanyama, Isa Kabenge, Joseph Jjagwe, Mukulu Diana, John Muyonga
Summary: Irrigation plays a crucial role in increasing eggplant yields amidst climate change, but the deficit irrigation level for optimal growth remains uncertain. This study examined the effect of deficit drip irrigation on eggplant performance and found that irrigating at 75% field capacity maximizes yield and water saving in sandy clay loam soil.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Andrea Marcone, Giorgio Impollonia, Michele Croci, Henri Blandinieres, Stefano Amaducci
Summary: This study used Sentinel-2 satellite images to estimate biophysical and biochemical parameters of spinach crops and found that canopy-level parameters were estimated more accurately. The estimation of the canopy-scale parameter AGB using MCARI showed the highest accuracy, while the estimation of the leaf-scale parameter LCC using NDWI showed the lowest accuracy. At the field scale, the validation results for AGB estimation using SR were the best.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Chengsheng Gong, Guangjun Guo, Baogui Pan, Changzhou Gao, Xianwei Zhu, Shubin Wang, Jinbing Liu, Weiping Diao
Summary: This study investigated the role of key cytokines and metabolic factors in the formation of plant-type traits in pepper. Through integrated analysis of RNA-seq and metabolite determination, the study identified the low content of gibberellin and high content of auxin as important factors causing plant dwarfing, and jasmonic acid as a metabolic factor affecting branch traits. A total of 131 candidate genes involved in metabolite synthesis and the formation of plant-type traits were identified.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yue Ma, Jia-Chen Hu, Yang Yu, Xin Cheng, Yan-Li Du, Qiang Zhao, Ji-Dao Du
Summary: Cadmium and drought stress significantly inhibited the growth of adzuki bean seedling plants, with the combined stress showing the highest degree of inhibition. Cd+D treatment reduced Cd accumulation in adzuki bean seedling roots. The treatment altered antioxidant enzyme activities and cell cycle phases in the roots, leading to changes in reactive oxygen species content.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Lucette Adet, Danae M. A. Rozendaal, Arthur Tapi, Pieter A. Zuidema, Philippe Vaast, Niels P. R. Anten
Summary: This study investigated the effects of water stress on cocoa trees and found that they are highly sensitive to water stress, with different genotypes exhibiting varied responses. Potassium application did not mitigate the negative effects of water stress. There was significant variation among genotypes in terms of leaf and crown traits, and positive interactions between genotype and irrigation were observed. These findings provide insights into the acclimation strategies of cocoa trees and can be useful for selecting drought-tolerant genotypes.
SCIENTIA HORTICULTURAE
(2024)