Journal
SCIENTIA HORTICULTURAE
Volume 120, Issue 3, Pages 379-385Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2008.11.035
Keywords
Somatic embryos; Total proteins; Glutelin; ABA; Sucrose; Arginine
Categories
Funding
- Tunisian Ministry of Scientific Research, Technology
- Development of Competencies
- International Atomic Energy Agency [RAF/5/049]
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Abscisic acid (ABA), arginine and sucrose were evaluated for their effects on the morphology, germination rates and protein content of date palm somatic embryos (SE). Different concentrations of these supplements in the culture medium were used. The comparative study of SE length and thickness between treated and untreated SE revealed no differences, except for ABA (20 mu M), which increased thickness. A decrease of water coil tent (WC) in favor of an increase in dry weight(DW) was observed in all treated SE, especially with Sucrose (90 g l (1)) and ABA (20 mu M). Only ABA (20 and 4 mu M) caused a proliferation rate Of the Cultures higher than those in the control. Although all the tested compounds increased protein content, ABA (20 mu M) was more effective in protein enrichment than arginine and Sucrose treatments. The SDS-PAGE protein profiles showed a significant difference between treated and untreated SE. A protein band of 22 kDa, identified as glutelin in a previous work, was accumulated after treatment with 20 mu M ABA or 3 mM arginine. These findings may contribute to further understanding of the mechanisms involved in the accumulation of specific storage proteins in several plants. (C) 2008 Elsevier B.V. All rights reserved.
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