Journal
SCIENTIA HORTICULTURAE
Volume 121, Issue 2, Pages 237-242Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2009.01.035
Keywords
DAMD; Genetic diversity; Punica granatum; RAPD; UPGMA
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Funding
- Department of Biotechnology, New Delhi
- Department of Science and Technology, New Delhi
- Council for Scientific and Industrial Research, New Delhi
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The genus Punica (Punicaceae) is distributed in Iran, Afghanistan, India and Mediterranean countries. Iran is considered to be its primary center of origin. In India Punica granatum is found in wild only in Western Himalayan regions comprising, Jammu and Kashmir, Himachal Pradesh and Uttarakhand states. However, there is little information available about the genetic variation present in pomegranates in the regions. In this paper we describe the use of DAMD and RAPID methods that generate the profiles, to study genetic diversity in wild genotypes of the P. granatum in India. Forty-nine accessions representing two regions of Western Himalaya were analyzed. Similarity coefficient value varied from 0.08 to 0.79 across different accessions. The results indicate that DAMD (97.08%) revealed more polymorphism in comparison to RAPD (93.72%). The results show that these methods are sufficiently informative to unravel the genetic variations in wild pomegranates in Western Himalayas. (c) 2009 Elsevier B.V. All rights reserved.
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