Article
Engineering, Marine
Awatef Ali, Soheir El Sherif, Jamila Abd Alla, Sahya Maulu, Ahmed A. Tantawy, Mahmoud F. K. Soliman, Mohamed M. Hassan, Montaser M. Hassan, Hala Saber Khalil
Summary: This study thoroughly described the morphology and complex differentiation of the reproductive system, spermatogenesis, and spermatophores structure of the blue crab. The research provided detailed insights into the fine structure of mature spermatozoa, contributing to a better understanding of the reproductive biology of the blue crab.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Plant Sciences
Jaindra Nath Tripathi, Valentine Otang Ntui, Leena Tripathi
Summary: Banana is an important food crop in the tropics, but it faces challenges from diseases and pests. Traditional breeding methods have limitations, leading to the exploration of genetic modification and genome editing tools. Transgenic approaches have been used to develop improved banana varieties with resistance to certain diseases and pests, but more research is needed. Genome editing, particularly CRISPR/Cas, offers precise modifications to the banana genome and has potential in improving banana varieties with enhanced resistance to various constraints.
Article
Plant Sciences
Baojian Chen, Martijn Fiers, Bas J. W. Dekkers, Lena Maas, G. Wilma van Esse, Gerco C. Angenent, Yang Zhao, Kim Boutilier
Summary: Research shows that exposing explants to growth regulator 2,4-D can induce somatic embryogenesis in Arabidopsis seeds, with a crucial role played by ABA in promoting this process by maintaining the seed maturation environment and regulating the expression of transcription factors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Cell Biology
Jin Sun, Weisheng Zheng, Wenqiang Liu, Xiaochen Kou, Yanhong Zhao, Zehang Liang, Lu Wang, Zihao Zhang, Jing Xiao, Rui Gao, Shaorong Gao, Cizhong Jiang
Summary: The study derived TSCs from blastocysts using different methods and analyzed the dynamics of transcription and DNA methylation, revealing the crucial role of FGF4 treatment in TSC formation. Specific DNA methylation remodeling processes and transcription factor networks play a key role in TSC pluripotency.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Pallavi Goel, Shivani Malpotra, Songyukta Shyam, Deepak Kumar, Manoj Kumar Singh, Prabhat Palta
Summary: There is a significant difference in the global miRNA profile between cloned and IVF embryos, which is manifested throughout the course of embryonic development. The differentially expressed miRNAs target biological processes, molecular functions, cellular components, and protein classes in cloned embryos compared to IVF embryos. Cloned embryos are affected in major pathways related to embryonic development more than IVF embryos.
Article
Genetics & Heredity
Daiki Shikata, Shogo Matoba, Masashi Hada, Akihiko Sakashita, Kimiko Inoue, Atsuo Ogura
Summary: In this study, researchers identified RLTR45-int subfamily of ERVK as a strong reprogramming barrier for SCNT. This reprogramming barrier not only suppressed the expression of RLTR45-int elements, but also inhibited the expression of nearby genes, further affecting the development and survival of embryos.
FRONTIERS IN GENETICS
(2022)
Article
Veterinary Sciences
Lian Cai, Sang-Hwan Hyun, Eunhye Kim
Summary: This study reveals the important role of stem cell factor (SCF) in the development of porcine embryos and the establishment of embryonic stem cells (ESCs). SCF treatment has no significant effect on the development potential and blastocyst formation rate of both fertilized and cloned porcine embryos, but significantly increases the rate of hatched blastocysts in embryos cloned with Cloud eGFP as donor cells. The SCF/c-kit signaling pathway may play a pivotal role in embryo implantation. Moreover, SCF treatment significantly enhances the efficiency of deriving ESCs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Plant Sciences
Phisouda Sivilay, Yong Eui Choi
Summary: Direct somatic embryogenesis occurs in the thin cell layers of zygotic embryos of Aesculus hippocastanum without the intervention of callus stage, and it is suppressed by the presence of 2,4-D.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Genetics & Heredity
Yanhui Kang, Wanfeng Li, Lifeng Zhang, Liwang Qi
Summary: The over-expression of LaCDKB1;2 was found to enhance the quality of Larix kaempferi somatic embryos and promote cell proliferation rate, resulting in an increased number of somatic embryos.
Article
Agriculture, Multidisciplinary
Elyeza Bakaze, Beloved Mensah Dzomeku, Jens-Norbert Wuensche
Summary: The study evaluated plant defense response in banana rhizome tissue against the banana weevil, with findings showing that susceptible variety experienced 30% damage compared to less than 5% in the tolerant variety. Plant defense substances in rhizome were correlated with larval damage and methyl jasmonate application induced higher defense potential. Tissue staining revealed increased deposition of defense compounds in response to pest attack.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Biochemical Research Methods
Shipra Goyal, Vijaya Chatterjee, Vishvas M. Kulkarni, Vishnu Bhat
Summary: This study successfully established cell suspension culture of Cenchrus ciliaris and achieved in vitro plant regeneration through induction of somatic embryogenesis. The method provided a potential source of competent cells for genetic transformation.
Article
Biotechnology & Applied Microbiology
Tanushree Chakraborty, K. Viswanatha Chaitanya, Nasim Akhtar
Summary: Cotyledon segments from germinated immature zygotic embryos of Pterocarpus santalinus were used for somatic embryogenesis. Different combinations of growth regulators were tested, and 2,4-D was found to be the most effective for embryogenic frequency. The number of somatic embryos and plantlets obtained varied with the concentrations of BAP and 2,4-D. 2,4-D and NAA, either alone or in combination with BAP, were found to significantly affect the embryonic potential of the cotyledon explant.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biochemistry & Molecular Biology
Tianjiao Zhang, Garry Wong
Summary: This study investigates the identity and distribution of piRNAs in human somatic tissues, revealing their potential role in neurodegenerative diseases, particularly Parkinson's disease. Several piRNAs are identified as potential biomarkers for Parkinson's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ah Reum Lee, Ji-Hoon Park, Sung Han Shim, Kwonho Hong, Hyeonwoo La, Kyung-Soon Park, Dong Ryul Lee
Summary: The study found that the activity of Rad 51 is lower in SCNT eggs and a significantly lower level of DSBs in SCNT embryos during reprogramming. Supplementation with RS-1 during the activation of SCNT embryos can increase RAD51 expression and DSB foci, thereby improving the efficiency of SCNT reprogramming. Through single-cell RNA-seq analysis, reactivation of genes and involvement of histone demethylase in reducing reprogramming-resistant regions were observed, which may contribute to overcoming developmental block and enhancing cell sources for regenerative medicine.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Pil-Soo Jeong, Hae-Jun Yang, Soo-Hyun Park, Min Ah Gwon, Ye Eun Joo, Min Ju Kim, Hyo-Gu Kang, Sanghoon Lee, Young-Ho Park, Bong-Seok Song, Sun-Uk Kim, Deog-Bon Koo, Bo-Woong Sim
Summary: Combining chaetocin and TSA can enhance development and maturation rates of porcine SCNT embryos by improving epigenetic reprogramming. The synergistic effect of the combined treatment is more effective in promoting blastocyst development compared to using chaetocin or TSA alone.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Nurzatil Sharleeza Mat Jalaluddin, Rofina Yasmin Othman, Jennifer Ann Harikrishna
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2019)
Article
Plant Sciences
Nee Kiew Poon, Chee How Teo, Rofina Yasmin Othman
JOURNAL OF GENERAL PLANT PATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Nazrin Abd-Aziz, Boon Chin Tan, Nur Ardiyana Rejab, Rofina Yasmin Othman, Norzulaani Khalid
MOLECULAR BIOTECHNOLOGY
(2020)
Review
Agriculture, Multidisciplinary
Sheue Ni Ong, Sima Taheri, Rofina Yasmin Othman, Chee How Teo
JOURNAL OF PLANT DISEASES AND PROTECTION
(2020)
Article
Biotechnology & Applied Microbiology
Xue Li Tan, Rofina Yasmin Othman, Chee How Teo
Article
Biotechnology & Applied Microbiology
Mohtaram Mahmoudieh, Mohamad Roff Mohd Noor, Jennifer Ann Harikrishna, Rofina Yasmin Othman
JOURNAL OF APPLIED GENETICS
(2020)
Article
Multidisciplinary Sciences
Ka Yun Tan, Avirup Dutta, Tze King Tan, Ranjeev Hari, Rofina Y. Othman, Siew Woh Choo
Article
Agriculture, Multidisciplinary
Narendren Rengasamy, Rofina Y. Othman, Hang S. Che, Jennifer A. Harikrishna
Summary: The combination of red, blue, and UV-A light proved to be the most effective in improving germination rates and yields for Stevia rebaudiana. While red and blue light combinations were effective for plant growth, supplemental UV-A irradiation significantly delayed flowering and enhanced yields. Overall, the use of red, blue, and UV-A light resulted in the best productivity for Stevia rebaudiana.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Genetics & Heredity
SiouNing Aileen See, Zee Hong Goh, Yen Yew Chan, Khai En Chong, Geok Yuan Annie Tan, Subha Bhassu, Rofina Yasmin Othman
Summary: Disease outbreak is a major challenge in shrimp aquaculture industry. This study developed a DPO-based multiplex PCR system for simultaneous detection of shrimp viruses. The system showed potential for rapid and effective screening of shrimp viruses in laboratory settings.
Letter
Environmental Sciences
Siew Woh Choo, Ju Lian Chong, Philippe Gaubert, Alice Catherine Hughes, Stephen O'Brien, Anne-Lise Chaber, Agostinho Antunes, Sara Platto, Nick Ching-Min Sun, Li Yu, Klaus-Peter Koepfli, Tulshi Laxmi Suwal, Mukesh Thakur, Stephan Ntie, Elisa Panjang, Jayaraj Vijaya Kumaran, Tariq Mahmood, Sean P. Heighton, Dago Dorji, Bi Sery Gonedele, Bryan Raveen Nelson, Chabi A. M. S. Djagoun, Ing Hoe Loh, Prativa Kaspal, Siim Pauklin, Toby Michelena, Hongxiang Zhu, Leonard Lipovich, Xuechen Tian, Siwei Deng, Christopher E. Mason, Jingyang Hu, Robert White, Nicholas S. Jakubovics, Wei Yee Wee, Tze King Tan, Kum Thong Wong, Steve Paterson, Ming Chen, Yixin Zhang, Rofina Yasmin Othman, Larry C. Brown, Bairong Shen, Guanghou Shui, Mia Yang Ang, Yunqi Zhao, Yongming Li, Bo Zhang, Cheng Tung Chong, Yu Meng, Aloysius Wong, Jianzhong Su, Hasmahzaiti Omar, Hua Shen, Choo Hock Tan, Hongyu Xu, Ian C. Paterson, Minyan Wang, Chee-Kai Chan, Siyuan Zhang, Avirup Dutta, Tay Sun Tee, Noel P. D. Juvigny-Khenafou, Naresh V. R. Mutha, Muhamad Afiq Aziz
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agronomy
Narendren Rengasamy, Rofina Yasmin Othman, Hang Seng Che, Jennifer Ann Harikrishna
Summary: This study found that photoperiod manipulation can increase productivity and energy use efficiency in indoor cultivation of Stevia rebaudiana plants in non-native environments. Continuous 16-hour photoperiod with supplemental red and blue light resulted in the highest biomass accumulation and metabolite yields.
Article
Environmental Sciences
T. Alinejad, S. Bhassu, F. B. Mustafa, R. Y. Othman
GLOBAL NEST JOURNAL
(2020)
Article
Agriculture, Multidisciplinary
Umaiyal Munusamy, Yusmin Mohd-Yusuf, Nadiya Akmal Baharum, Kamilatulhusna Zaidi, Rofina Yasmin Othman
PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES
(2019)
Article
Parasitology
W. M. Al-Madhagi, N. M. Hashim, N. A. A. Ali, R. Othman
TROPICAL BIOMEDICINE
(2019)
Article
Biotechnology & Applied Microbiology
Umaiyal Munusamy, Yusmin Mohd-Yusuf, Kamilatulhusna Zaidi, Nadiya Akmal Baharum, Rofina Yasmin Othman
RESEARCH JOURNAL OF BIOTECHNOLOGY
(2019)
Article
Horticulture
Peng Jia, Rui Yan, Yuan Wang, Feng-huan Gao, Yang Liu, Qing-long Dong, Hao-an Luan, Xue-mei Zhang, Han Li, Su-ping Guo, Guo-hui Qi
Summary: The KNOX gene plays crucial roles in plant development, and this study identified 19 KNOX gene members in kiwifruit. One of the genes, AcKNOX11, is selectively expressed in flower buds and shoots, and its expression can be induced by ABA and melatonin. Functional analysis revealed that AcKNOX11 delays flowering, reduces plant height, alters leaf shape, and inhibits seed germination through ABA-melatonin interaction.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Dan Hua, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Huan-Huan Chen, Ning-Wei Lai, Lin-Tong Yang, Xin Ye, Li -Song Chen
Summary: This study revealed that nitrogen deficiency can lead to increased production of reactive oxygen species (ROS) and methylglyoxal (MG), but also enhances the ability of leaves and roots to detoxify these molecules. The effects of nitrogen deficiency on ROS and MG generation and their detoxification systems were found to be different in leaves and roots, with roots being more affected.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Shenghan Yang, Chonlong Chio, Wensheng Qin, Yanxi Pei, Guangpeng Pei, Yuen Zhu, Hua Li
Summary: This study evaluates the immobilization and deactivation of pathogen-produced enzymes and toxic metabolites by biochar in tomato Fusarium wilt and finds that biochar can effectively prevent disease occurrence.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Deborah Pagliaccia, Michelle Ortiz, Michael Rodriguez, Sophia Abbott, Agustina De Francesco, Madison Amador, Valeria Lavagi, Benjamin Maki, Francesca Hopkins, Jonathan Kaplan, Samantha Ying, Georgios Vidalakis
Summary: This study investigates the effectiveness of organic soil amendments (bokashi, biochar, and their combination) in promoting soil health, nutrient availability, and plant growth. The results show that these amendments can significantly alter soil parameters and have positive effects on soil and plant health.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yu-Jeong Kwon, Min Jeong Hong, Minkyu Park, Dawon Jeon, Gah-Hyun Lim, Sungyul Chang, Dong-Hwan Kim, Jin-Baek Kim, Jundae Lee, Yeong Deuk Jo
Summary: Flowing LOCUS T (FT)-like genes play crucial roles in flowering induction in plants. Pepper FT-like genes are mostly expressed in leaves and interact with FLOWERING LOCUS D, promoting or delaying flowering. The unique evolutionary process of pepper FT-like genes provides insights for improving productivity.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Ziwen Su, Juan Yan, Binbin Zhang, Meng Sun, Zhixiang Cai, Zhijun Shen, Ruijuan Ma, Mingliang Yu
Summary: Bagging is a crucial cultivation measure that affects the quality of peach fruit by regulating exposure to light. This study investigated the effects of bagging on the content of phenolic compounds in peach fruit and identified the most sensitive compounds to bagging treatment. The study also explored the regulatory role of bagging on phenolic synthetic-related genes. The results showed that bagging affected the accumulation of different phenolics in different peach cultivars and light intensity played a significant role in the accumulation of anthocyanins and other phenolic substances in peach fruit. Additionally, the expression of structural genes and transcription factors related to phenolic synthesis was regulated by bagging and light conditions. These findings provide a foundation for understanding the regulation mechanism of light on the synthesis of phenolic compounds in peach fruit.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yanfang Zhang, Xinzhi Cui, Zhilei Du, Xiulian Li, Bangdi Liu, Meng Liu, Xiangquan Zeng, Fengjun Guo, Xinguang Fan, Shuyang Sun
Summary: In this study, melatonin treatment effectively improved the aroma quality of apricots during low temperature storage by maintaining firmness and soluble solids content, inhibiting ethylene production, and regulating enzyme activities related to aroma synthesis.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Sang-Mo Kang, Shifa Shaffique, Md. Injamum-Ul-Hoque, Sarah Owdah Alomrani, Yong-Sung Park, In-Jung Lee
Summary: Salinity is a global environmental problem that affects the physiology and morphology of plants. Melatonin has been found to improve photosynthesis, antioxidant activities, and seedling characteristics in milk thistle plants exposed to salinity stress, thereby enhancing their salt tolerance.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Nadia Borgini, Haifa Benmoussa, Mohamed Ghrab, Mehdi Ben Mimoun
Summary: This study investigates the agroclimatic requirements of Prunus species cultivars growing in warm areas using Partial Least Squares regression. The findings reveal that the chilling and heat requirements of the cultivars appear discontinuous, with overlaps or transition periods between the two phases. The warm mean temperatures occurring during the chilling period are the main determinant of the flowering of the studied cultivars.
SCIENTIA HORTICULTURAE
(2024)
Review
Horticulture
Kayise Hypercia Maseko, Thierry Regnier, Belinda Meiring, Obiro Cuthbert Wokadala, Tonna Ashim Anyasi
Summary: Bananas are a significant tropical fruit with diverse cultivars, providing essential minerals, vitamins, and phytochemicals. The Cavendish group is preferred, but identifying suitable cultivars and maturity stages is crucial for various applications.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Godfrey Ouma, Joshua Wanyama, Isa Kabenge, Joseph Jjagwe, Mukulu Diana, John Muyonga
Summary: Irrigation plays a crucial role in increasing eggplant yields amidst climate change, but the deficit irrigation level for optimal growth remains uncertain. This study examined the effect of deficit drip irrigation on eggplant performance and found that irrigating at 75% field capacity maximizes yield and water saving in sandy clay loam soil.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Andrea Marcone, Giorgio Impollonia, Michele Croci, Henri Blandinieres, Stefano Amaducci
Summary: This study used Sentinel-2 satellite images to estimate biophysical and biochemical parameters of spinach crops and found that canopy-level parameters were estimated more accurately. The estimation of the canopy-scale parameter AGB using MCARI showed the highest accuracy, while the estimation of the leaf-scale parameter LCC using NDWI showed the lowest accuracy. At the field scale, the validation results for AGB estimation using SR were the best.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Chengsheng Gong, Guangjun Guo, Baogui Pan, Changzhou Gao, Xianwei Zhu, Shubin Wang, Jinbing Liu, Weiping Diao
Summary: This study investigated the role of key cytokines and metabolic factors in the formation of plant-type traits in pepper. Through integrated analysis of RNA-seq and metabolite determination, the study identified the low content of gibberellin and high content of auxin as important factors causing plant dwarfing, and jasmonic acid as a metabolic factor affecting branch traits. A total of 131 candidate genes involved in metabolite synthesis and the formation of plant-type traits were identified.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yue Ma, Jia-Chen Hu, Yang Yu, Xin Cheng, Yan-Li Du, Qiang Zhao, Ji-Dao Du
Summary: Cadmium and drought stress significantly inhibited the growth of adzuki bean seedling plants, with the combined stress showing the highest degree of inhibition. Cd+D treatment reduced Cd accumulation in adzuki bean seedling roots. The treatment altered antioxidant enzyme activities and cell cycle phases in the roots, leading to changes in reactive oxygen species content.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Lucette Adet, Danae M. A. Rozendaal, Arthur Tapi, Pieter A. Zuidema, Philippe Vaast, Niels P. R. Anten
Summary: This study investigated the effects of water stress on cocoa trees and found that they are highly sensitive to water stress, with different genotypes exhibiting varied responses. Potassium application did not mitigate the negative effects of water stress. There was significant variation among genotypes in terms of leaf and crown traits, and positive interactions between genotype and irrigation were observed. These findings provide insights into the acclimation strategies of cocoa trees and can be useful for selecting drought-tolerant genotypes.
SCIENTIA HORTICULTURAE
(2024)
