Journal
SCIENCEASIA
Volume 40, Issue 2, Pages 141-144Publisher
THAILANDS NATL SCIENCE & TECHNOLOGY DEVELOPMENT AGENCY
DOI: 10.2306/scienceasia1513-1874.2014.40.141
Keywords
medicinal mushroom; agaricales; soxhlet extraction; phenolic compound
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Funding
- higher Education Research Promotion and National Research University Project of Thailand, Office of the higher Education Commission
- Centre of Excellence-Oil Palm, Kasetsart University
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Phellinus linteus (PL), a mushroom known as krathin phiman in Thai, has been used as a traditional medicine in the treatment of various cancers. Hispolon obtained from PL ethanol extracts is a promising anti-cancer compound. In this study, ethanol concentration and extraction time were varied for a soxhlet extraction. The content of hispolon in a crude extract was determined by high performance liquid chromatography with a UV detector. The presence of hispolon in the crude extract was demonstrated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with daughter ion scanned mode. The results showed that both hispolon and Thai PL have a similar parent ion at m/z 218.91 and daughter ions at m/z 176, 160, 135.
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