Article
Chemistry, Multidisciplinary
Yasunari Matsuzaka, Shinji Kusakawa, Yoshihiro Uesawa, Yoji Sato, Mitsutoshi Satoh
Summary: This study utilizes deep learning to detect cancer-cell contamination in cell cultures, with optimized models for better classification and identification of disease cell types, showing a wide range of potential applications.
APPLIED SCIENCES-BASEL
(2021)
Article
Plant Sciences
Misato Ota, Toshiaki Makino
Summary: This study reviews the effectiveness of processing licorice root and finds that the traditional Chinese medicine method is more effective than the Japanese Kampo medicine method. Heating honey as a drug adjuvant can enhance the immunostimulatory effects of licorice root.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Andres F. Vallejo, James Davies, Amit Grover, Ching-Hsuan Tsai, Robert Jepras, Marta E. Polak, Jonathan West
Summary: Constellation sequencing (Constellation-Seq) is a molecular transcriptome filter that delivers two orders of magnitude sensitivity gains, reduces data sparsity, and sequencing costs. This technique reliably measures changes in gene expression, making it suitable for studying rare cell populations and exploring their biology with extreme resolution.
Article
Nutrition & Dietetics
Thayane Ferreira da Costa Fernandes, Naiane Beatriz Ferreira, Rafaela Ramos Campagnoli, Fabio da Silva Gomes, Filipe Braga, Isabel Antunes David, Isabela Lobo
Summary: Ultra-processed food products (UPF) have been associated with non-communicable diseases. The addictive nature of UPF and aggressive marketing strategies have created an emotional connection between UPF and consumers, supporting global consumption. Modulating emotional reactions to UPF is important in changing consumer behavior. A study using electroencephalography found that textual warnings about the health risks of consuming UPF changed emotional responses and increased motivation to avoid UPF.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
Raja Annapooranan, Sunil Suresh Jeyakumar, Robert J. Chambers, Rong Long, Shengqiang Cai
Summary: This work explores the fabrication of pressure sensitive adhesives (PSAs) using liquid crystal elastomers (LCEs) known for their excellent dissipation properties. The adhesive properties of the PSAs are evaluated and found to show significant rate and temperature dependence. The unique properties of LCE, such as soft elasticity and non-linear viscoelasticity, contribute to the high-rate dependent adhesion. The study demonstrates the potential of LCE as a new category of adhesives with special properties for novel engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Nutrition & Dietetics
Adam Drewnowski
Summary: This study examined plant-based beverages (PBBs) marketed as milks in the US and found that most of them contained added salt and sugar, with a majority falling under the category of ultra-processed foods. Coconut PBBs were noted for their high saturated fat content, while almond and pea products had higher nutrient density scores.
ADVANCES IN NUTRITION
(2021)
Article
Cell Biology
Xiangnan Zhang, Shuo Liang, Enze Wang, Ning Tao
Summary: The study aimed to build cellular aggregates of TS/A and normal fibroblasts or CAFs, and verify the value of this model in drug screening and the effect of CD44 on aggregate formation. The results showed that cellular aggregates were successfully formed in the improved culture medium, CAFs promoted the formation of cancer cell aggregates, eugenol had value in drug screening, and CD44 had an important effect on aggregate formation.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Spectroscopy
Jun-Hu Cheng, Xinxue Zhang, Ji Ma, Da-Wen Sun
Summary: In this study, a label-free fluorescent aptasensor based on polythymidine (poly(T))-templated copper nanoclusters (CuNCs) was developed for sensitive detection of tropomyosin (TM) in processed shrimp products. The aptasensor achieved a linear range of 0.1 to 50 μg/mL, a low limit of detection of 0.0489 μg/mL, and excellent recovery percentage in the complex food matrix, providing a new approach for food safety assurance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Nutrition & Dietetics
Anna Kokkinopoulou, Niki Katsiki, Ioannis Pagkalos, Nikolaos E. Rodopaios, Alexandra-Aikaterini Koulouri, Eleni Vasara, Sousana K. Papadopoulou, Petros Skepastianos, Maria Hassapidou, Anthony G. Kafatos
Summary: This study aimed to investigate the consumption of ultra-processed foods (UPFs) and its association with metabolic syndrome (MetS) in a Christian Orthodox church religious fasting population in Greece. The results showed that fasters consumed more unprocessed or minimally processed foods, such as seafood, fish, soy products, and fresh fruits, and also consumed more processed foods, such as fried potatoes and olives. Fasters with MetS had a higher intake of UPFs compared to fasters without MetS.
Article
Nutrition & Dietetics
Florencia Alcaire, Leticia Vidal, Leandro Machin, Lucia Antunez, Ana Gimenez, Maria Rosa Curutchet, Gaston Ares
Summary: The information included on food packages plays a crucial role in influencing consumer perceptions and decisions. This study examines the use of health-related cues on processed and ultra-processed food packages in Uruguay to inform policy making.
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Public, Environmental & Occupational Health
Natalia Oliveira, Daniela Silva Canella
Summary: The consumption of ultra-processed beverages is associated with health problems, while the purchase of minimally processed beverages declined. With an increase in the proportion of ultra-processed foods and beverages in the diet, the volume of ultra-processed beverage purchases rose and minimally processed beverages declined.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Chemistry, Analytical
Song Nie, Tyler Greer, Reid O'Brien Johnson, Xiaojing Zheng, Albert Torri, Ning Li
Summary: LC-MS is a powerful tool for analyzing host cell proteins during antibody drug development, but the dynamic range between therapeutic antibodies and HCPs poses challenges. The ULTLB method enables sensitive and simple analysis of numerous HCPs without the need for cumbersome enrichment steps.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiaohui Yu, Haopeng Zhang, Yufei Wang, Xiaoshan Fan, Zibiao Li, Xu Zhang, Tianxi Liu
Summary: In this study, novel conductive composite hydrogels with high stretchability, ultra-softness, excellent conductivity, and good self-healing ability were developed. The hydrogels were formed by incorporating polyaniline nanoparticles (PANI-NPs) into poly(poly(ethylene glycol) methacrylate-co-acrylic acid) (P(PEG-co-AA)) scaffolds through dynamically electrostatic interactions and hydrogen bonds. The PANI-NPs served as conductive fillers, while the enhanced interactions between PANI-NPs and P(PEG-co-AA) matrix imparted high stretchability, low modulus, excellent elasticity, and fast self-healing ability to the hydrogel. The introduction of a binary solvent system also allowed for ideal anti-freezing properties. Composite hydrogel-based sensors were proposed, demonstrating states-independent properties, low detection limit, high linearity, and excellent anti-fatigue performance.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Food Science & Technology
Leticia Vidal, Geronimo Brunet, Isabel Bove, Alejandra Girona, Dario Fuletti, Gaston Ares
Summary: This study investigated the automatic mental associations and affective reactions of parents of children under 12 months old towards ultraprocessed products. The majority of parents had negative associations with certain ultraprocessed products, while some exhibited positive affective reactions towards others. Parents of infants aged 1-4 months, as well as those with low educational level and socioeconomic status, were more likely to have a positive affective reaction towards ultraprocessed products. Additionally, beliefs about feeding practices significantly influenced parents' associations with ultraprocessed products.
FOOD QUALITY AND PREFERENCE
(2021)
Article
Food Science & Technology
Lucia Perez, Lucia Antunez, Tobias Otterbring, Gaston Ares
Summary: This study assessed the impact of nutritional warnings on mental associations raised by advertisements featuring ultra-processed products. The results suggest that nutritional warnings may be an effective strategy to increase consumer awareness of the nutritional characteristics of processed products without affecting the ability of advertisements to convey their intended message.
FOOD QUALITY AND PREFERENCE
(2022)
Article
Biochemistry & Molecular Biology
Kei Saito, Tohru Fujiwara, Shunsuke Hatta, Masanobu Morita, Koya Ono, Chie Suzuki, Noriko Fukuhara, Yasushi Onishi, Yukio Nakamura, Shin Kawamata, Ritsuko Shimizu, Masayuki Yamamoto, Hideo Harigae
MOLECULAR AND CELLULAR BIOLOGY
(2019)
Article
Cell & Tissue Engineering
Jung-Hyun Kim, Alex Alderton, Jeremy M. Crook, Nissim Benvenisty, Catharina Brandsten, Meri Firpo, Peter W. Harrison, Shin Kawamata, Eihachiro Kawase, Andreas Kurtz, Jeanne F. Loring, Tenneille Ludwig, Jennifer Man, Joanne C. Mountford, Marc L. Turner, Steve Oh, Lygia da Veiga Pereira, Patricia Pranke, Michael Sheldon, Rachel Steeg, Stephen Sullivan, Michael Yaffe, Qi Zhou, Glyn N. Stacey
Article
Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Shinji Kusakawa, Takuya Kuroda, Mayumi Futamura, Mitsuhide Ogawa, Hidemi Mochizuki, Eri Kikkawa, Hatsue Furukawa, Masato Nagaoka, Yoji Sato
Summary: The MEASURE study aims to standardize the tumorigenicity evaluation of hPSC-derived CTPs, finding that the HEC assay and MACS can improve sensitivity to residual undifferentiated hPSCs. Multisite experiments confirmed the effectiveness of these methods in detecting low levels of undifferentiated cells.
Article
Multidisciplinary Sciences
Takamasa Hirai, Ken Kono, Rumi Sawada, Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama, Akifumi Matsuyama, Naoya Koizumi, Naoki Utoguchi, Hiroyuki Mizuguchi, Yoji Sato
Summary: The study demonstrates that the Ad vector is the most efficient in detecting undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells. It also shows that the Ad vector selectively eliminates hiPSC-NPCs, enabling sensitive detection of hiPSCs. This cytotoxic viral vector could enhance the quality and safety of hiPSCs-NPCs for therapeutic use.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Keiko Sugai, Miho Sumida, Tomoko Shofuda, Ryo Yamaguchi, Takashi Tamura, Tsuneo Kohzuki, Takayuki Abe, Reo Shibata, Yasuhiro Kamata, Shuhei Ito, Toshiki Okubo, Osahiko Tsuji, Satoshi Nori, Narihito Nagoshi, Shinya Yamanaka, Shin Kawamata, Yonehiro Kanemura, Masaya Nakamura, Hideyuki Okano
Summary: Our group has conducted extensive studies on the use of human induced pluripotent cell-derived neural stem/progenitor cell grafts in models of spinal cord injury. We are preparing to initiate a first-in-human clinical study in subacute SCI patients. The study aims to assess safety and preliminary evidence of impact on neurological function and quality-of-life outcomes.
REGENERATIVE THERAPY
(2021)
Review
Cell & Tissue Engineering
Yoshiteru Kamiyama, Yoichi Naritomi, Yuu Moriya, Syunsuke Yamamoto, Tsukasa Kitahashi, Toshihiko Maekawa, Masahiro Yahata, Takeshi Hanada, Asako Uchiyama, Akari Noumaru, Yoshiyuki Koga, Tomoaki Higuchi, Masahiko Ito, Hiroyuki Komatsu, Sosuke Miyoshi, Sadaaki Kimura, Nobuhiro Umeda, Eriko Fujita, Naoko Tanaka, Taku Sugita, Satoru Takayama, Akihiko Kurogi, Satoshi Yasuda, Yoji Sato
Summary: Information on the biodistribution of cell therapy products is crucial for predicting and assessing their efficacy and toxicity. Understanding regulatory requirements, implementation status, and analytical methods is necessary for conducting BD studies. The results of this review will serve as a useful resource for implementing BD studies.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Takako Yamamoto, Yoji Sato, Satoshi Yasuda, Masayuki Shikamura, Takashi Tamura, Chiemi Takenaka, Naoko Takasu, Masaki Nomura, Hiromi Dohi, Masayo Takahashi, Michiko Mandai, Yonehiro Kanemura, Masaya Nakamura, Hideyuki Okano, Shin Kawamata
Summary: This study suggests that genetic testing can predict abnormal tissue formation after transplantation of iPSC derivatives for cell therapy. While no positive correlation was found between the presence of SNVs/deletions and abnormal tissue formation, a copy number greater than 3 was strongly correlated. Additionally, CNV analysis may be useful in predicting abnormal tissue formation and reducing the need for tumorigenicity tests.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Orthopedics
Takehiko Matsushita, Tomoyuki Matsumoto, Daisuke Araki, Kanto Nagai, Yuichi Hoshino, Takahiro Niikura, Atsuhiko Kawamoto, Masahiro J. Go, Shin Kawamata, Masanori Fukushima, Ryosuke Kuroda
Summary: The study examines the safety and effectiveness of IK-01 (CaReSTM) in treating focal cartilage injury of the knee. Results show that IK-01 significantly improves clinical scores and pain levels in patients, and MRI evaluations indicate similarity with surrounding cartilage.
ASIA-PACIFIC JOURNAL OF SPORT MEDICINE ARTHROSCOPY REHABILITATION AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yoshitaka Shirasago, Hidesuke Fukazawa, Shotaro Nagase, Yoshimi Shimizu, Tomoharu Mizukami, Takaji Wakita, Tetsuro Suzuki, Hideki Tani, Masuo Kondoh, Takuya Kuroda, Satoshi Yasuda, Yoji Sato, Kentaro Hanada, Masayoshi Fukasawa
Summary: The entry of the hepatitis C virus into host cells is a complex process mediated by various host factors. Through studying a highly infectious HCV-JFH1-tau substrain, researchers discovered that a single mutation in the E2 glycoprotein, M706L, was essential for infecting non-permissive cells. This mutant virus was able to use CLDN1, CLDN6, and CLDN9 to infect cells other than hepatocytes. Additionally, induction pluripotent stem cells were found to be susceptible to HCV infection, suggesting that HCV may change its receptor requirement through a single amino acid mutation to infect non-hepatic cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Takamasa Hirai, Ken Kono, Shinji Kusakawa, Satoshi Yasuda, Rumi Sawada, Akihiko Morishita, Shinko Hata, Atsushi Wakita, Takayasu Kageyama, Ryo Takahashi, Sono Watanabe, Norihiko Shiraishi, Yoji Sato
Summary: This study demonstrated the reproducibility of the cellular immortality test in detecting tumorigenic cellular impurities, as well as its ability to detect immortalized cells with different growth characteristics, contributing to the safety and quality of hCTPs.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Connie L. Chen, Louise Delsing, Mick D. Fellows, Gabor Foldes, Shinji Kusakawa, Lucilia Pereira Mouries, Yoji Sato
Summary: The study evaluated the sensitivity and reproducibility of the highly efficient culture (HEC) assay for detecting residual undifferentiated human pluripotent stem cells (hPSCs) in cell therapy products. All facilities were able to detect spiked hiPSCs in hiPSC-derived cardiomyocytes, with repeatability being the main factor affecting reproducibility. The results show that the HEC assay is highly sensitive and can be widely used for tumorigenicity evaluation of hPSC-derived cell therapy products.
REGENERATIVE MEDICINE
(2023)
Article
Toxicology
Yuji Yamashita, Tamio Sato, Kaori Noishiki, Kinji Kobayashi, Asako Uchiyama, Hiroyuki Izumi, Takashi Tamura, Masayuki Shikamura, Yu Oinishi, Miho Saito, Shin Kawamata
Summary: Based on survival data, it appears reasonable to terminate tumorigenicity study at 52 weeks of age in NOG mice.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2021)
Article
Genetics & Heredity
Takumi Miura, Satoshi Yasuda, Yoji Sato
Summary: Next-generation sequencing (NGS) has revolutionized genetic/genomic research, particularly in detecting mutations associated with disease risk and evaluating cell substrates for biopharmaceuticals. This study developed a simple method to estimate the limit of detection (LOD) for low-frequency somatic mutations using whole-exome sequencing (WES) by NGS. Results showed that with 15 Gbp or more sequencing data, WES was able to detect mutations with allele frequencies between 5% and 10%, providing a rationale for setting a cutoff threshold in interpreting somatic genetic mutations.
Article
Multidisciplinary Sciences
Fumiya Ohashi, Shigeru Miyagawa, Satoshi Yasuda, Takumi Miura, Takuya Kuroda, Masayoshi Itoh, Hideya Kawaji, Emiko Ito, Shohei Yoshida, Atsuhiro Saito, Tadashi Sameshima, Jun Kawais, Yoshiki Sawa, Yoji Sato
SCIENTIFIC REPORTS
(2019)
