Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 479, Issue -, Pages 227-232Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2014.01.120
Keywords
Caffeine; Surface water; ELISA; Anthropogenic markers
Categories
Funding
- European Funds through COMPETE
- National Funds through the Portuguese Science Foundation (Fundacao para a Ciencia e Tecnologia, FCT) [PEst-C/MAR/LA0017/2013]
- FCT [SFRH/BD/74430/2010, SFRH/BPD/80315/2011]
- BAM
- Spanish Ministry of Science and Innovation [RYC-2010-05634]
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This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100 mu g L-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between
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