4.7 Article

Plant tolerance to diesel minimizes its impact on soil microbial characteristics during rhizoremediation of diesel-contaminated soils

Journal

SCIENCE OF THE TOTAL ENVIRONMENT
Volume 409, Issue 19, Pages 4087-4093

Publisher

ELSEVIER
DOI: 10.1016/j.scitotenv.2011.06.025

Keywords

Lolium perenne; n-alkanes; Soil microbial community; Soil pollution; Soil quality; Trifolium repens

Funding

  1. [ETORTEK-BERRILUR-III-IE09-242]
  2. [UPV/EHU-GV IT-299-07]
  3. [PLANTOX-UE09+/48]

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Soil contamination due to petroleum-derived products is an important environmental problem. We assessed the impacts of diesel oil on plants (Trifolium repens and Lolium perenne) and soil microbial community characteristics within the context of the rhizoremediation of contaminated soils. For this purpose, a diesel fuel spill on a grassland soil was simulated under pot conditions at a dose of 12,000 mg diesel kg(-1) DW soil. Thirty days after diesel addition, T. repens (white clover) and L perenne (perennial ryegrass) were sown in the pots and grown under greenhouse conditions (temperature 25/18 degrees C day/night, relative humidity 60/80% day/night and a photosynthetic photon flux density of 400 mu mol photon m(-2) s(-1)) for 5 months. A parallel set of unplanted pots was also included. Concentrations of n-alkanes in soil were determined as an indicator of diesel degradation. Seedling germination, plant growth, maximal photochemical efficiency of photosystem II (F-v/F-m), pigment composition and lipophylic antioxidant content were determined to assess the impacts of diesel on the studied plants. Soil microbial community characteristics, such as enzyme and community-level physiological profiles, were also determined and used to calculate the soil quality index (SQI). The presence of plants had a stimulatory effect on soil microbial activity. L perenne was far more tolerant to diesel contamination than T. repens. Diesel contamination affected soil microbial characteristics, although its impact was less pronounced in the rhizosphere of L perenne. Rhizoremediation with T. repens and L perenne resulted in a similar reduction of total n-alkanes concentration. However, values of the soil microbial parameters and the SQI showed that the more tolerant species (L perenne) was able to better maintain its rhizosphere characteristics when growing in diesel-contaminated soil, suggesting a better soil health. We concluded that plant tolerance is of crucial importance for the recovery of soil health during rhizoremediation of contaminated soils. (C) 2011 Elsevier B.V. All rights reserved.

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