Article
Chemistry, Analytical
Prajal Chettri, Kalathur Mohan Ganesh, Sahashransu Satyajeet Mahapatra, A. S. Vishwanathan, Shailesh Srivastava
Summary: Optofluidic chips are cost-effective platforms for miniaturized devices to perform biochemical reactions at a microscale. In this study, a dye reduction-based electron-transfer activity monitoring (DREAM) assay was adopted to rapidly assess bacterial activity in bioreactors. A three-layered PMMA-based optofluidic chip coupled with a detection system consisting of an LED source and a photodiode was used, and data acquisition was done through a user-friendly GUI. The study also compared the performance of a photodiode-based device with expensive spectrophotometers for optoelectronic automation.
ANALYTICAL METHODS
(2023)
Article
Green & Sustainable Science & Technology
Ning Duan, Jiangmin Xiong, Qi Feng, Lihui Wang, Fan Yang, Hua Ding
Summary: This paper proposes a domestic sewage collection model for rural areas and develops an operable village sewage treatment plan in Jiangxia District, Wuhan City. The plan effectively reduces pollution in the Yangtze River and contributes to the restoration of its ecological environment.
Article
Chemistry, Multidisciplinary
Akito Nakagawa, Marissa K. Cooper, Maria Kost-Alimova, James Berstler, Binglan Yu, Lorenzo Berra, Elizabeth S. Klings, Mary S. Huang, Matthew M. Heeney, Donald B. Bloch, Warren M. Zapol
Summary: The study developed a high-throughput sickling assay to screen for compounds that can inhibit sickling in sickle cell disease (SCD). The compounds SNS-314 mesylate and voxelotor were found to successfully prevent sickling, potentially expanding the therapeutic options for SCD.
Article
Biochemical Research Methods
Weiruo Zhang, Irene Li, Nathan E. Reticker-Flynn, Zinaida Good, Serena Chang, Nikolay Samusik, Saumyaa Saumyaa, Yuanyuan Li, Xin Zhou, Rachel Liang, Christina S. Kong, Quynh-Thu Le, Andrew J. Gentles, John B. Sunwoo, Garry P. Nolan, Edgar G. Engleman, Sylvia K. Plevritis
Summary: CELESTA is an unsupervised machine learning algorithm that identifies cell types based on markers and spatial information. By applying CELESTA to imaging data of colorectal cancer and head and neck squamous cell carcinoma, researchers identified tissue architecture associated with lymph node metastasis and validated their findings through cell-cell interaction analysis.
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Review
Biochemistry & Molecular Biology
Wencke Walter, Claudia Haferlach, Niroshan Nadarajah, Ines Schmidts, Constanze Kuhn, Wolfgang Kern, Torsten Haferlach
Summary: Artificial intelligence is becoming indispensable in the healthcare sector, with applications including image processing, disease classification, and information recognition. It can help improve diagnostic accuracy and treatment success rates.
Article
Plant Sciences
Fahad Al-Qurainy, Mohamed Tarroum, Salim Khan, Mohammad Nadeem, Abdel-Rhman Z. Gaafar, Saleh Alansi, Norah S. Alfarraj
Summary: The genome size and phytochemical compounds in the leaves and callus of Abrus precatorius were evaluated. The genetic stability and chemical compositions of callus and leaves were analyzed, and the compounds were identified using GC-MS.
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Article
Immunology
Adriana Victoria Sabljic, Silvina Sonia Bombicino, Juan Ignacio Marfia, Luciano Lucas Guerra, Alberto Penas Steinhardt, Natalia Ines Faccinetti, Ruben Francisco Iacono, Edgardo Poskus, Aldana Trabucchi, Silvina Noemi Valdez
Summary: A novel flow cytometric microsphere-based immunoassay (FloCMIA) using recombinant human proinsulin (PI) as an alternative to the Radioligand Binding Assay (RBA) was developed in this study. The method showed fair ability to distinguish between samples from diabetic patients and normal controls, with a prevalence of 50% for PAA in the diabetic patient population. The assay demonstrated good performance, wide dynamic range, and the potential for multiplex immunoassays for the detection of autoantibodies in Type 1 Diabetes Mellitus and other autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Nicolas Fissolo, Laura Calvo-Barreiro, Herena Eixarch, Ursula Boschert, Carmen Espejo, Xavier Montalban, Manuel Comabella
Summary: Cladribine exerts regulatory effects on the proliferation and apoptosis of lymphocyte subsets while its impact on immune cell activation is mediated through both DCK-dependent and independent pathways. It shows cytotoxicity on monocytes and dendritic cells, affecting the activation of monocytes but not the maturation of dendritic cells.
Article
Medicine, Research & Experimental
Dinh Thi Thai Ha, Kittirat Glab-ampai, Pornchai Rojsitthisak, Opa Vajragupta
Summary: This study successfully constructed CXCR4 overexpressing HEK293T cells and verified the feasibility of using non-radioactive binding assay with these cells. The assay can serve as an alternative tool for screening anticancer compounds targeting CXCR4 and is essential for mechanism validation in drug discovery.
Article
Biodiversity Conservation
Thomas Weisse, Jitka Jezberova, Michael Moser
Summary: The study measured the feeding and growth rates of the sessile peritrich ciliate Vorticella similis on seven picocyanobacterial strains and their accompanying bacterial flora, confirming that V. similis is an indiscriminative suspension feeder whose ingestion rates depend on picoplankton abundance and biomass. The results provide valuable information on the feeding activity of V. similis and highlight the importance of peritrich ciliates in water purification processes.
ECOLOGICAL INDICATORS
(2021)
Review
Medical Laboratory Technology
Nadia Guell, Pablo Mozas, Alba Jimenez-Rueda, Milos Miljkovic, Jordi Junca, Marc Sorigue
Summary: This study examines the methodological and conceptual aspects of flow cytometric classification of leukemic B-cell lymphoproliferative disorders (B-LPDs) and discusses the challenges in integrating different biological data points, the under examination of misclassification costs, and the impact of the lack of a true diagnostic gold standard. The study concludes that without addressing these considerations, external reproducibility and interobserver agreement cannot be achieved, and the field cannot progress without a true gold standard.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Article
Medical Laboratory Technology
Wouter H. G. Hubens, Tiago Maie, Matthis Schnitker, Ledio Bocova, Deepika Puri, Martina Wessiepe, Jan Kramer, Lothar Rink, Steffen Koschmieder, Ivan G. Costa, Wolfgang Wagner
Summary: Cell-type specific DNA methylation can be used to accurately count different leukocyte subsets in blood. In this study, the authors developed targeted DNAm assays and used digital droplet PCR to measure DNAm levels in venous blood and dried blood samples. The results showed good correlations between epigenetic estimates and conventional cell counting methods, and dried blood samples facilitated self-sampling for easier testing accessibility.
CLINICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Toshimi Nakajima, Mao Kuragano, Makoto Yamada, Ryo Sugimoto
Summary: This study compared the contribution of submarine groundwater discharge (SGD) to river nutrient budgets at nearshore and embayment scales, and found that SGD-derived nutrients become more important at larger spatial scales.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Fan Liu, Lei Zhang, Chongyang Zhang, Ziguang Chen, Jingguang Li
Summary: NO2 emissions from wall-mounted gas stoves used for household heating have become a significant source of indoor pollution in Chinese urban areas. The high indoor concentration of NO2 poses potential health risks to residents. It is urgently necessary to establish relevant regulations and implement emission reduction technologies to reduce NO2 emissions from wall-mounted gas stoves.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Letter
Environmental Sciences
Hans Peter H. Arp, Raoul Wolf, Sarah E. Hale, Sivani Baskaran, Juliane Gluege, Martin Scheringer, Xenia Trier, Ian T. Cousins, Harrie Timmer, Roberta Hofman-Caris, Anna Lennquist, Andre D. Bannink, Gerard J. Stroomberg, Rosa M. A. Sjerps, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Daniel Zahn, Herve Gallard, Tobias Mohr, Ivo Schliebner, Michael Neumann
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Philomina Onyedikachi Peter, Binessi Edouard Ifon, Francois Nkinahamira, Kayode Hassan Lasisi, Jiangwei Li, Anyi Hu, Chang-Ping Yu
Summary: This study investigates the relationship between dissolved organic matter (DOM) and Rare Earth Elements (REEs) in sediments from Yundang Lagoon, China. The results show four distinct fluorescent components, with protein-like substances being the most prevalent. Additionally, the total fluorescence intensity and LREE concentrations exhibit a synchronized increase from Outer to Inner to Songbai Lake core sediments. The findings demonstrate a strong correlation between DOM content and pollution levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Surya Gupta, Pasquale Borrelli, Panos Panagos, Christine Alewell
Summary: The objective of this study is to incorporate soil hydraulic properties into the erodibility factor (K) of USLE-type models. By modifying and improving the existing equations for soil texture and permeability, the study successfully included information on saturated hydraulic conductivity (Ksat) into the calculation of K factor. Using the Random Forest machine learning algorithm, two independent K factor maps with different spatial resolutions were generated. The results show that the decrease in K factor values has a positive impact on the modeling of soil erosion rates.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Jesmin Akter, Wendy J. M. Smith, Yawen Liu, Ilho Kim, Stuart L. Simpson, Phong Thai, Asja Korajkic, Warish Ahmed
Summary: The choice of workflow in wastewater surveillance has a significant impact on SARS-CoV-2 concentrations, while having minimal effects on HF183 and no effect on HAdV 40/41 concentrations. Certain components in the workflow can be interchangeable, but factors such as buffer type, chloroform, and homogenization speed can affect the recovery of viruses and bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Yu Luo, Xueting Yang, Diwei Wang, Hongmei Xu, Hongai Zhang, Shasha Huang, Qiyuan Wang, Ningning Zhang, Junji Cao, Zhenxing Shen
Summary: Atmospheric PM2.5, which can generate reactive oxygen species (ROS), is associated with cardiorespiratory morbidity and mortality. The study found that both the mass concentration of PM2.5 and the DTT activity were higher during the heating season than during the nonheating season. Combustion sources were the primary contributors to DTT activity during the heating season, while secondary formation dominated during the nonheating season. The study also revealed that biomass burning had the highest inherent oxidation potential among all sources investigated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Erin L. Murphy, Leah R. Gerber, Chelsea M. Rochman, Beth Polidoro
Summary: Plastic pollution has devastating consequences for marine organisms. This study uses a trait-based framework to develop a vulnerability index for marine mammals, seabirds, and sea turtles in Hawai'i. The index ranks 63 study species based on their vulnerability to macroplastic pollution, providing valuable information for species monitoring and management priorities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Kenji Maurice, Amelia Bourceret, Sami Youssef, Stephane Boivin, Liam Laurent-Webb, Coraline Damasio, Hassan Boukcim, Marc-Andre Selosse, Marc Ducousso
Summary: Growing pressure from climate change and agricultural land use is destabilizing soil microbial community interactions. Little is known about microbial community resistance and adaptation to disturbances, hindering our understanding of recovery latency and implications for ecosystem functioning. This study found that anthropic disturbance and natural disturbance have different effects on the topology and stability of soil microbial networks.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Yunhao Li, Yali Feng, Haoran Li, Yisong Yao, Chenglong Xu, Jinrong Ju, Ruiyu Ma, Haoyu Wang, Shiwei Jiang
Summary: Deep-sea mining poses a serious threat to marine ecosystems and human health by disturbing sediment and transmitting metal ions through the food chain. This study developed a new regenerative adsorption material, OMN@SA, which effectively removes metal ions. The adsorption mechanism and performance of the material for metal ion fixation were investigated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Antonio Medici, Margherita Lavorgna, Marina Isidori, Chiara Russo, Elena Orlo, Giovanni Luongo, Giovanni Di Fabio, Armando Zarrelli
Summary: Valsartan, a widely used antihypertensive drug, has been detected in high concentrations in surface waters due to its unchanged excretion and incomplete degradation in wastewater treatment plants. This study investigated the degradation of valsartan and identified 14 degradation byproducts. The acute and chronic toxicity of these byproducts were evaluated in key organisms in the freshwater trophic chain.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Jiang Lin, Lianbao Chi, Qing Yuan, Busu Li, Mingbao Feng
Summary: This study investigated the photodegradation behavior and product formation of two representative pharmaceuticals in simulated estuary water. The study found that the formed transformation products of these pharmaceuticals have potential toxicity on marine organisms, including oxidative stress and damage to cellular components.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Hua Fang, Dongdong Jiang, Ye He, Siyi Wu, Yuehong Li, Ziqi Zhang, Haoting Chen, Zixin Zheng, Yan Sun, Wenxiang Wang
Summary: This study revealed that exposure to lower levels of air pollutants led to decreased pregnancy rates, with PM10, NO2, SO2, and CO emerging as the four most prominent pollutants. Individuals aged 35 and above exhibited heightened susceptibility to pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Ali Shaan Manzoor Ghumman, Rashid Shamsuddin, Amin Abbasi, Mohaira Ahmad, Yoshiaki Yoshida, Abdul Sami, Hamad Almohamadi
Summary: In this study, inverse vulcanized polysulfides (IVP) were synthesized by reacting molten sulfur with 4-vinyl benzyl chloride, and then functionalized using N-methyl D-glucamine (NMDG). The functionalized IVP showed a high mercury adsorption capacity and a machine learning model was developed to predict the amount of mercury removed. Furthermore, the functionalized IVP can be regenerated and reused, providing a sustainable and cost-effective adsorbent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Rita Bonfiglio, Renata Sisto, Stefano Casciardi, Valeria Palumbo, Maria Paola Scioli, Erica Giacobbi, Francesca Servadei, Gerry Melino, Alessandro Mauriello, Manuel Scimeca
Summary: This study investigated the presence of aluminum in human colon cancer samples and its potential association with biological processes involved in cancer progression. Aluminum was found in tumor areas of 24% of patients and was associated with epithelial to mesenchymal transition (EMT) and cell death. Additional analyses revealed higher tumor mutational burden and mutations in genes related to EMT and apoptosis in aluminum-positive colon cancers. Understanding the molecular mechanisms of aluminum toxicity may improve strategies for the management of colon cancer patients.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)