Article
Nanoscience & Nanotechnology
Lea A. Lumper, Gerald J. K. Schaffar, Michael Sommerauer, Verena Maier-Kiener
Summary: This study compares confocal laser scanning and scanning electron microscopy for material characterization at elevated temperatures. Investigations on two alloys provide important insights into their imaging capabilities, limitations, and general information obtained, which contribute to the advancement of high-temperature materials science.
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING
(2023)
Article
Engineering, Chemical
Emily W. Tow, Behzad Rad, Robert Kostecki
Summary: This study investigates the behavior of biofouling on water reuse membranes using confocal microscopy. Through in situ monitoring and image analysis, the growth and removal processes of biofilms and the production of extracellular polymeric substances are revealed. The results provide important insights for the development of improved techniques for biofouling mitigation.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Review
Polymer Science
Aref Abbasi Moud
Summary: In this brief overview, we discussed the applications of confocal laser scanning microscopy and Raman imaging spectroscopy in studying polymer blends. The results show that these techniques can be used to evaluate various components and properties of polymer mixes.
Article
Optics
Hamed Qazvini, Hamid Latifi, Amir Asadollahi
Summary: One of the main challenges in scanning microscopy is to increase the scanning speed without compromising the spatial resolution of the image. This paper presents a solution using Lissajous scanning and phase modulation techniques to achieve higher imaging speeds while maintaining resolution. Experimental results show that this approach can significantly improve both temporal and spatial resolution.
Article
Chemistry, Multidisciplinary
Yuhan Li, Milo S. P. Shaffer
Summary: This study demonstrates the novel use of confocal microscopy for high-resolution characterization, in situ laser reduction, and versatile patterning of graphene oxide (GO) films. The reduction process can be attributed to thermal transfer and accumulation, which can be controlled over multiple length scales. This technique allows for the fabrication of conductive reduced GO films and 3D microscale architectures, and provides a valuable tool for well-controlled and scalable fabrication.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Optics
Xiaohan Du, Camilo Florian, Craig B. Arnold
Summary: Existing auto-focusing methods in laser processing typically include two independent modules, one for surface detection and another for [Formula: see text]-axis adjustment. To alleviate the slow processing speed, the researchers developed a single-lens approach that combines surface detection and focus control in a dual-beam setup. The approach provides instantaneous surface tracking and accelerates the axial alignment process, offering great potential for enhancing the speed of advanced manufacturing processes in three-dimensional space.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Zhiping Li, Liangshun Luo, Yanqing Su, Binbin Wang, Liang Wang, Tong Liu, Mengjia Yao, Chen Liu, Jingjie Guo, Hengzhi Fu
Summary: In this paper, the transformation behavior from the α phase to γ lamellae in γ-TiAl alloys during the cooling process was investigated using high-temperature laser scanning confocal microscopy (HTLSCM). The nucleation and growth behaviors of γ lamellae were studied at moderate cooling rates, and the effect of cooling rate on the hardness of the alloys was also examined.
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
(2022)
Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Materials Science, Multidisciplinary
Haoxin Liu, Ming Zhong, Yang Shen, Zhanjun Wang, Somnath Basu, Cong Wang
Summary: Salient phase transformations, including successive delta-gamma-martensite transformations, were observed in the coarse-grained heat-affected zone of P92 heat-resistant steel using a confocal scanning laser microscope. The final microstructure consisted of transformed martensites and retained delta-ferrites. The growth rate of martensite laths ranged from 86 to 354 μm/s, indicating a wide range of rates. The fast cooling rate prevented complete transformation of delta-ferrite to gamma-austenite due to the non-equilibrium condition.
MATERIALS CHARACTERIZATION
(2023)
Article
Chemistry, Physical
Tianyu Yan, Xinyu Wang, Siting Liu, Dawei Fan, Xinyi Xu, Qi Zeng, Hui Xie, Xiaoli Yang, Shouping Zhu, Xiaopeng Ma, Zhen Yuan, Xueli Chen
Summary: A novel NIR-II CLSM system has been developed using inexpensive SiPM for multicolor biological imaging in vivo, achieving spatial resolution close to the diffraction limit and very low fluorescence detection sensitivity. Experiments in mice demonstrated comparable multicolor imaging performance in both NIR-I and NIR-II windows.
Article
Materials Science, Multidisciplinary
Evgenii Vasilev, Jie Wang, Marko Knezevic
Summary: This study introduces a new metric to quantitatively assess fracture surfaces, successfully distinguishing between fracture in fatigue and fracture in monotonic tension. The metric is also sensitive to the ductility of tested alloys and the presence of critical defects.
MATERIALS CHARACTERIZATION
(2022)
Article
Chemistry, Applied
Pranita Mhaske, Asgar Farahnaky, Stefan Kasapis
Summary: The efficacy of commercially available image analysis software in quantifying 3D images captured with CLSM was evaluated. The study focused on the phase behavior of a mixed system of agarose and gelatin that was allowed to gel by controlled cooling. Results showed that imaging software could effectively model the phase behavior of the gels.
FOOD HYDROCOLLOIDS
(2023)
Article
Biology
Kathrin Malfeld, Nina Armbrecht, Holger A. Volk, Thomas Lenarz, Verena Scheper
Summary: Recent research has found that auditory synaptopathies play a crucial role in sensorineural hearing loss, and cochlear implant procedures may lead to physiological hearing loss in patients with residual hearing. The study developed a new method to examine the inner ear at the synapse level, aiming to better understand the cascade of hearing pathologies with the goal of developing targeted therapeutics.
Article
Plant Sciences
Jan Knoblauch, Rainer Waadt, Asaph B. Cousins, Hans-Henning Kunz
Summary: Researchers have established three microscopy protocols to determine the in situ volume of chloroplasts using chlorophyll fluorescence, embedded CFP markers, and calculations from serial block-face scanning electron microscopy. The obtained data were validated by comparing them with mutant lines. The study also found that the size of guard cell plastids is not governed by the same division machinery as mesophyll plastids.
Article
Materials Science, Multidisciplinary
Xin Li, Fei Gao, Junhong Jiao, Guangming Cao, Yong Wang, Zhenyu Liu
Summary: The experimental results demonstrate that the solidification mode of 316H austenitic stainless steel is ferrite-austenite type (FA mode) regardless of the cooling rate. A peritectic reaction occurs between ferrite and liquid phase during solidification. The higher cooling rate leads to a higher volume fraction of 6-ferrite.
MATERIALS CHARACTERIZATION
(2021)
