Article
Cell Biology
Toneisha Stubbs, James I. Bingman, Jason Besse, Kirk Mykytyn
Summary: Primary cilia are important for neuronal functions and their dysfunction is linked to ciliopathies. Bardet-Biedl syndrome proteins play a crucial role in trafficking G protein-coupled receptors (GPCRs) in neuronal cilia, and their absence leads to abnormal localization of signaling proteins in the brain.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ricardo Fradique, Erika Causa, Clara Delahousse, Jurij Kotar, Laetitia Pinte, Ludovic Vallier, Marta Vila-Gonzalez, Pietro Cicuta
Summary: Cilia density, distribution, and beating frequency are crucial properties of airway epithelial tissues. This article introduces a novel and open-source method for fully characterizing cilia beating frequency and motile cilia coverage in an automated manner without any user intervention. The method successfully differentiates between different coverage densities, identifies small patches of cilia in larger fields of view, and provides a comprehensive characterization of cilia beating frequency in all moving areas.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Chemistry, Analytical
Yanrong Wen, Ge Ge, Dan Xie, Zhen Liu
Summary: A comprehensive analytical platform is lacking for tracking and quantifying cell migration behaviors and molecular dynamics at the single-cell level. In this study, an integrated single living cell analysis platform was developed to address this limitation. It enables long-term observation of migration behavioral phenotypes in single cells and simultaneous analysis of signaling proteins and complexes, providing insights into the molecular mechanism and phenotypes in cell migration.
ANALYTICAL CHEMISTRY
(2023)
Review
Physiology
Himanshu Malhotra, Cassandra L. Barnes, Peter D. Calvert
Summary: Retinal photoreceptors are neurons that convert light into electrical signals for transmission to the brain. The functions of photoreceptors are divided into three specialized compartments- the outer segment, inner segment, and pre-synaptic terminal. Disruption of this compartmentalization can lead to devastating blinding diseases.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lance Lee, Lawrence E. Ostrowski
Summary: Over the past two decades, our understanding of motile cilia and their role in disease has greatly increased, with critical information and insight coming from the analysis of mouse models. Mouse models have made significant contributions to genetic and cell biological studies of motile cilia, and have been at the forefront of research on mammalian motile cilia and PCD pathogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Editorial Material
Multidisciplinary Sciences
Jeffrey A. Farrell
Summary: CellOracle, a computational tool, can predict the interaction of gene networks in programming cell identity during embryonic development. This tool will contribute to a better understanding of the regulation of development.
Editorial Material
Multidisciplinary Sciences
Alain Chedotal
Summary: The GPC3-Unc5 protein complex is found to regulate cell migration in embryos and cancer, indicating a similarity in the migration mechanisms of developing neurons and metastatic cancer cells.
Article
Cell Biology
Yong Ha Youn, Shirui Hou, Chang-Chih Wu, Daisuke Kawauchi, Brent A. Orr, GilesW. Robinson, David Finkelstein, Makoto M. Taketo, Richard J. Gilbertson, Martine F. Roussel, Young-Goo Han
Summary: This study reveals that primary cilia have different functions in different types of tumors and uncovers the molecular mechanisms underlying their involvement in tumorigenesis.
GENES & DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Huai-Bin Hu, Zeng-Qing Song, Guang-Ping Song, Sen Li, Hai-Qing Tu, Min Wu, Yu-Cheng Zhang, Jin-Feng Yuan, Ting-Ting Li, Pei-Yao Li, Yu-Ling Xu, Xiao-Lin Shen, Qiu-Ying Han, Ai-Ling Li, Tao Zhou, Jerold Chun, Xue-Min Zhang, Hui-Yan Li
Summary: Dynamic assembly and disassembly of primary cilia play critical roles in tissue development and homeostasis. The authors have identified lysophosphatidic acid (LPA) as a physiological extracellular factor that initiates cilia disassembly through both YAP/TAZ mediated transcription and calcium/calmodulin mediated activation of Aurora A.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Amir Mohammad Rahimi, Mingfang Cai, Sigrid Hoyer-Fender
Summary: In this study, the embryonic mouse fibroblast cell line NIH3T3 was utilized to establish homogeneous cell lines for research on molecular signatures and pathways associated with ciliation.
Editorial Material
Multidisciplinary Sciences
Till Klecker, Benedikt Westermann
Summary: Mitochondria, essential organelles in cells, are successfully inherited during cell division through three types of interaction with filaments of actin protein.
Article
Cell Biology
Min Huang, Xinlong Kong, Zaiming Tang, Zaisheng Lin, Ruida He, Muqing Cao, Xiujuan Zhang
Summary: The protein TCHP plays a crucial role in cell cycle progression by disassembling cilia, and its depletion leads to cell cycle arrest in G(0) phase, which cannot be reversed by blocking cilia assembly. Additionally, TCHP-induced cell cycle arrest is not mediated by centrosome surveillance mechanism, but requires inhibition of Rb or both Rb and p53 signaling pathways to reverse the phenotype.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biology
Benjamin Barsi-Rhyne, Aashish Manglik, Mark von Zastrow
Summary: Beta-arrestins are important regulators of cellular signaling, involving in the desensitization and endocytosis of GPCRs. Two different modes of endocytic activity have been discovered, one dependent on the C-terminus and the other dependent on the C-lobe of beta-arrestins. These modes are triggered in a receptor-specific manner and have opposite effects on the signaling output of receptors.
Article
Biology
Hyeran Helen Jeon, Jessica Kang, Jiahui (Madelaine) Li, Douglas Kim, Gongsheng Yuan, Nicolette Almer, Min Liu, Shuying Yang
Summary: The effects of IFT80 deletion in osteocytes on orthodontic and physiological bone remodeling were investigated, and it was found that the deletion did not significantly impact these processes.
Article
Cardiac & Cardiovascular Systems
Saejeong Park, Zhiyuan Ma, Georgia Zarkada, Irinna Papangeli, Sarin Paluri, Nour Nazo, Felix Rivera-Molina, Derek Toomre, Sudarshan Rajagopal, Hyung J. Chun
Summary: This study discovered the localization of beta-arrestins in primary cilia of endothelial cells and their interaction with the flagellar transport protein complex component IFT81. Knockdown of beta-arrestins resulted in weakened shear stress response and disrupted interaction with the type II bone morphogenetic protein receptor, leading to impaired endothelial shear response. Mice with endothelial specific deletion of beta-arrestins exhibited impaired retinal angiogenesis.
PULMONARY CIRCULATION
(2022)
