Article
Medicine, Research & Experimental
Defu Chen, David W. Nauen, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Ang Li, Honghua Guan, Carmen Kut, Kaisorn L. Chaichana, Chetan Bettegowda, Alfredo Quinones-Hinojosa, Xingde Li
Summary: This study systematically investigated the feasibility of using multiphoton microscopy for rapid histopathological assessment of label-and processing-free surgical specimens, focusing on infiltrating glioma. The results showed that multiphoton microscopy robustly captures diagnostic features of glioma and deep learning can assist with image recognition.
Article
Chemistry, Multidisciplinary
Yingxian Zhang, Jincheng Zhong, Hui Cheng, Jie Huang, Zhenhui Li, Chi Zhang, Zhiang Gao, Zhourui Xu, Gaixia Xu, Ping Qiu, Ke Wang
Summary: By detailed measurements and comparison of the effective attenuation length (l(e)) in different excitation wavelengths, this study demonstrates that 3-photon microscopy at 1700 nm enables the deepest penetration in brain imaging in vivo.
NANOSCALE ADVANCES
(2023)
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemical Research Methods
Ryan T. Lang, Bryan Q. Spring
Summary: The study utilized Ti:Sapphire femtosecond lasers with a wavelength-tunable source of pulsed excitation to demonstrate peak molecular brightness spectra for eight Alexa Fluor dyes, showing deeper imaging depth and improved photostability at longer wavelengths. The introduction of new generations of pulsed lasers with wider tunable ranges enhances the accuracy and stability of microscopy imaging.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Multidisciplinary Sciences
Karolina Vernmark, Annika Knutsen, Per Loftas, Xiao-Feng Sun, Mona Pathak
Summary: This study investigated the survival outcomes of rectal cancer patients with mucinous adenocarcinoma (MAC) and non-mucinous adenocarcinoma (NMAC) after adjuvant chemotherapy. The results showed that MAC patients had better overall survival and a trend towards improved cancer-specific survival after chemotherapy. In contrast, there was no significant improvement in survival for NMAC patients.
Article
Oncology
Yu-Wen Zhou, Yi-Xiu Long, Ye Chen, Ji-Yan Liu, Dan Pu, Jia-Yan Huang, Feng Bi, Qiu Li, Hong-Feng Gou, Meng Qiu
Summary: The study compared the efficacy of first-line bevacizumab plus chemotherapy and cetuximab plus chemotherapy in metastatic colorectal cancer patients with mucinous adenocarcinoma or mucinous component. The results showed that bevacizumab-based chemotherapy was associated with significantly better overall survival in patients with mucinous adenocarcinoma or mucinous component, regardless of tumor sites.
Article
Endocrinology & Metabolism
Tadayuki Yoshitake, Seymour Rosen, Lucas C. Cahill, Simon Lamothe, Ashley Ward, James G. Fujimoto
Summary: Nonlinear microscopy (NLM) is a promising technique for rapid evaluation of bone pathology. It enables visualization of normal bone architecture, articular cartilage, and pathological bone features, but training is required for accurate evaluation. Further studies are needed to assess diagnostic performance and address evaluation challenges with NLM.
Article
Multidisciplinary Sciences
Michael Hamersky, Khushi Tekale, L. Matthew Winfree, Mathew J. M. Rowan, Lindsey Seldin
Summary: Understanding cell behaviors is crucial for disease intervention, and optimizing imaging methods is necessary for observing cell dynamics. This study presents a customized microscope stage insert for inverted confocal microscopes, facilitating intravital imaging in intact animals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Pathology
Yehe Liu, Richard M. Levenson, Michael W. Jenkins
Summary: This article explains the technical basis of slide-free microscopy, reviews common slide-free optical microscopy techniques, and discusses the opportunities and challenges involved in clinical implementation.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Biochemical Research Methods
J. Brunet, C. L. Walsh, W. L. Wagner, A. Bellier, C. Werlein, S. Marussi, D. D. Jonigk, S. E. Verleden, M. Ackermann, Peter D. Lee, Paul Tafforeau
Summary: The authors present a protocol for preparing and mounting whole human organs for X-ray hierarchical phase-contrast tomography, resulting in high-resolution images. Imaging across different scales is crucial for understanding organ morphology and pathophysiological changes. The protocol enables scanning of large soft-tissue samples, including intact human organs, with enhanced contrast and reduced sample movement and bubble formation.
Article
Multidisciplinary Sciences
Dengbo Ji, Jinying Jia, Xinxin Cui, Zhaowei Li, Aiwen Wu
Summary: Mucinous colorectal adenocarcinoma (MC) has poor response to chemotherapy and prognosis compared to non-MC (NMC). Fibroblast activation protein (FAP) is upregulated in MC patients and negatively correlated with prognosis and therapeutic outcomes in CRC patients. FAP promotes CRC cell growth, invasion, metastasis, and chemoresistance. Myosin phosphatase Rho-interacting protein (MPRIP) is a direct interacting protein of FAP. FAP influences chemotherapy efficiency and prognosis by promoting crucial CRC functions and inducing recruitment of tumor-associated macrophages (TAMs) and M2 polarization through the Rho/Hippo/YAP signaling pathway. Knockdown of FAP reverses tumorigenicity and chemoresistance in CRC cells. FAP may serve as a marker for prognosis and therapeutic target to overcome chemoresistance in MC patients.
Article
Biochemistry & Molecular Biology
Sammy Hermans, Jacob Pilon, Dennis Eschweiler, Johannes Stegmaier, Carmen A. H. Severens-Rijvers, Salwan Al-Nasiry, Marc van Zandvoort, Dimitrios Kapsokalyvas
Summary: Pre-eclampsia is a severe complication in pregnancy with limited diagnostic and therapeutic options, and there is controversy surrounding its etiology and phenotypes. Using multiphoton microscopy (MPM) imaging, the native placental three-dimensional morphology can be visualized with subcellular resolution, allowing for the identification of various imaging targets such as trophoblast organization, villous tree structure, syncytial knots, fibrosis, and vascular networks. Preliminary data suggests that pre-eclampsia is characterized by increased syncytial knot density, paddle-like villous sprouts, abnormal villous volume-to-surface ratio, and decreased vascular density. Quantifying 3D microscopic images shows potential for phenotyping pre-eclampsia in placental villous tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Benedetto Neri, Roberto Mancone, Luca Savino, Sara Schiavone, Vincenzo Formica, Francesca Pizzi, Silvia Salvatori, Michelangela Mossa, Stefano Migliozzi, Mariasofia Fiorillo, Cristina Morelli, Alessandro Moscardelli, Elisabetta Lolli, Emma Calabrese, Giuseppe S. S. Sica, Giovanni Monteleone, Livia Biancone
Summary: Chronic active inflammation is a known risk factor for colorectal cancer (CRC) in inflammatory bowel disease (IBD), while the adenoma-carcinoma sequence appears to be associated with sporadic CRC. Mucinous and signet-ring cell adenocarcinomas have been reported to have worse prognosis. In this study, the frequency and characteristics of these two types of adenocarcinomas were investigated in IBD patients compared to age-matched non-IBD Controls.
Article
Multidisciplinary Sciences
Juo-Nang Liao, Wei-Liang Chen, Chao-Yuan Lo, Man-Hong Lai, Huang-Lung Tsai, Yu-Ming Chang
Summary: In this study, a nondestructive method using two-photon fluorescence and second-harmonic generation imaging was demonstrated to quantify starch granules within chloroplasts of intact leaves across a day-night cycle. The results provided micron scale spatial resolution of starch distribution within leaves and showed starch circadian patterns consistent with previous studies. This method is a potential tool for revealing the real-time heterogeneity of starch circadian rhythm in leaf cells without the need for destructive sample preparation.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Kseniya S. Shin, Shuaiqian Men, Angel Wong, Colburn Cobb-Bruno, Eleanor Y. Chen, Dan Fu
Summary: A multimodal quantitative imaging approach combining SRS, TPF, and SHG microscopy can provide useful diagnostic information for bone tissue, with the potential to separate bone cancer-associated diagnostic categories with an average accuracy of 92%.
ANALYTICAL CHEMISTRY
(2022)