Review
Biochemistry & Molecular Biology
Anatoly N. Vereshchagin, Nikita A. Frolov, Ksenia S. Egorova, Marina M. Seitkalieva, Valentine P. Ananikov
Summary: Quaternary ammonium compounds (QACs) are well-known cationic biocides with a broad spectrum of antimicrobial activity, used in various products from personal care to disinfectants. Research focuses on developing new types of QACs and ILs to meet the increasing demand for efficient antimicrobials in modern society.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Songtai Gao, Yujun Sun, Zhong Lu, Nan Jiang, Huaiying Yao
Summary: Antibiotics overuse and misuse contribute to the emergence of multidrug-resistant bacterial strains and biofilm formation. This study screened out a combination of quaternary ammonium compound PDDA and Cu2+ as a promising antibacterial agent against Staphylococcus aureus. The combination showed strong synergistic antibacterial activity by disrupting membrane function, generating intracellular reactive oxygen, promoting Cu2+ uptake, and reducing biofilm extracellular matrix. In vitro biocompatibility studies demonstrated low cytotoxicity and hemolysis ratio. This work provides a novel and efficient strategy to combat planktonic and biofilm S. aureus infections.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Fateme Eskandari, Hossein Mofidi, Bahar Asheghi, Fateme Mohammadi, Ahmad Gholami
Summary: Nowadays, there is a growing concern about the inadequacy of new antimicrobials and the rise of antimicrobial resistance. In this study, the antimicrobial properties of zinc oxide nanoparticles (ZnONPs) were improved by coupling them with imidazolium (IM@ZnONPs). The results showed that IM@ZnONPs exhibited stronger antibacterial activities, especially against antimicrobial-resistant pathogens, compared to ZnONPs and zinc ions.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Allergy
Nicolas Migueres, Charlotte Debaille, Jolanta Walusiak-Skorupa, Agnieszka Lipinska-Ojrzanowska, Xavier Munoz, Vera van Kampen, Hille Suojalehto, Katri Suuronen, Martin Seed, Sewon Lee, Catherine Rifflart, Julien Godet, Frederic de Blay, Olivier Vandenplas
Summary: This study found that QAC-induced OA is associated with a highly eosinophilic pattern of airway response and provides further evidence supporting the sensitizing potential of QACs. The findings highlight the heterogeneous nature of the pathobiologic pathways involved in OA caused by LMW agents.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Infectious Diseases
Andrea T. Fessler, Anissa D. Scholtzek, Angela R. Schug, Barbara Kohn, Christiane Weingart, Anne-Kathrin Schink, Astrid Bethe, Antina Luebke-Becker, Stefan Schwarz
Summary: A study of 114 Staphylococcus isolates from companion animals revealed variations in susceptibility to antimicrobial agents, with a proportion of isolates carrying resistance genes. However, no signs of biocide resistance were detected at present.
Article
Chemistry, Multidisciplinary
Josisleine Recalde Allaion, Karina Ghougassian Barrionuevo, Maria Jose Grande Burgos, Antonio Galvez, Bernadette Dora Gombossy de Melo Franco
Summary: This study evaluated the tolerance of Staphylococcus aureus isolated from Minas artisanal cheeses to various biocides, as well as their resistance genes and enterotoxin production. The results showed that a significant percentage of isolates were resistant to at least one biocide and carried antibiotic resistance genes and enterotoxin genes. This highlights the importance of proper hygiene monitoring programs to ensure the safety of these products.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Farhana Parvin, Md. Arifur Rahman, Anand K. Deva, Karen Vickery, Honghua Hu
Summary: Staphylococcus aureus biofilms exhibit resistance to antibiotics and disinfectants, which is attributed to changes in the bacterial cell wall. Proteins involved in cell wall synthesis are upregulated in biofilms compared to planktonic organisms. Furthermore, the width of the cell wall and peptidoglycan production increase with biofilm culture duration and dehydration. Disinfectant tolerance is highest in dry surface biofilms, followed by hydrated biofilms, and lowest in planktonic bacteria, indicating that changes in the cell wall may contribute to the biocide resistance of S. aureus biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Nikita A. Frolov, Ksenia A. Fedoseeva, Karl A. Hansford, Anatoly N. Vereshchagin
Summary: Novel phenyl-based bis-quaternary ammonium compounds showed potent broad-spectrum biocidal activity against bacterial and fungal pathogens and have the potential to replace existing household sanitizers.
Article
Infectious Diseases
Emily M. Meredith, Lauren T. Harven, Andrew D. Berti
Summary: This study compared the effects of different mechanisms of tolerance induction on the effectiveness of antibiotics against Staphylococcus aureus. It was found that each mechanism of tolerance rendered at least one antibiotic ineffective, and each antibiotic was rendered ineffective by at least one mechanism of tolerance. Further research is needed to evaluate additional antibiotics, combination therapy, and different tolerance inducers.
Article
Chemistry, Medicinal
Nikita Frolov, Elena Detusheva, Nadezhda Fursova, Irina Ostashevskaya, Anatoly Vereshchagin
Summary: The study demonstrates that quaternary ammonium compounds based on pyridine structures with aromatic spacers have high antibacterial activity against clinically significant microorganisms, show synergistic effects in antiseptic formulations, and exhibit good antibiofilm properties.
Article
Chemistry, Physical
Kullapa Chanawanno, Pumis Thuptimdang, Suchada Chantrapromma, Hoong-Kun Fun
Summary: A new class of quaternary ammonium compounds (QACs) with two different spacer lengths was developed and tested for antibacterial activity. The compounds with a longer spacer showed broad bactericidal effect against both Gram-negative and Gram-positive bacteria, with substantial anti-Methicillin-Resistant Staphylococcus aureus (anti-MRSA) activity. The impact of these compounds on MRSA can be increased by varying the length of their spacers.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Microbiology
Li Shen, Jiao Zhang, Yao Chen, Lulin Rao, Xinyi Wang, Huilin Zhao, Bingjie Wang, Yanghua Xiao, Jingyi Yu, Yanlei Xu, Junhong Shi, Weihua Han, Zengqiang Song, Fangyou Yu
Summary: In this study, a novel small-molecule compound CY-158-11 was synthesized and found to have inhibitory effects on the biofilm formation of Staphylococcus aureus. The subinhibitory concentration of CY-158-11 did not affect bacterial growth and showed no toxicity to cells. The compound effectively prevented initial attachment, repressed PIA production, and suppressed genes involved in biofilm formation. Overall, CY-158-11 inhibits biofilm formation in S. aureus by inhibiting cell adhesion and the expression of icaA gene related to PIA production.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Niclas Nordholt, Kate O'Hara, Ute Resch-Genger, Mark A. T. Blaskovich, Bastian Ruehle, Frank Schreiber
Summary: Quaternary ammonium compounds (QACs) are widely used in disinfectants and preservatives, but their mode-of-action and resistance mechanisms in bacteria are not fully understood. Researchers synthesized a fluorescent QAC, NBD-DDA, and demonstrated its usefulness in molecular and single-cell studies. The results showed that NBD-DDA has comparable antimicrobial activity to widely used QACs, and provided insights into its mode-of-action and resistance mechanisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Chemistry, Medicinal
Evgeniya A. Saverina, Nikita A. Frolov, Olga A. Kamanina, Vyacheslav A. Arlyapov, Anatoly N. Vereshchagin, Valentine P. Ananikov
Summary: In the past, the focus was mainly on individual antibacterial activity, but now there is a shift towards affecting more complex cellular frameworks and using multiple strategies to combat biofilms at different stages. Quaternary ammonium compounds (QACs) play a key role in these treatments. This review summarizes the literature data on the effectiveness of using commercially available and newly synthesized QACs, as well as synergistic treatment techniques based on them. It also discusses the development and application of antimicrobial coatings to prevent biofilm formation on various surfaces.
ACS INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Chao Zhang, Bingshao Liang, Zhile Xiong, Zhuwei Liang, Hao Cai, Huamin Zhong, Yongqiang Xie, Yuanwei Xie, Xiaochun Liu, Shiying Xie, Fangjun Lan, Zhenwen Zhou
Summary: The purpose of this study was to investigate the distribution of biocide resistance genes among S. aureus isolates from school-age children in Guangzhou, and to explore the relationship between these genes and clonal complex genotypes as well as biofilm formation in biocide resistance. The results showed that mepA was the most prevalent biocide resistance gene, while qacG gene was not detected. The qacA/B and mupA genes were highly associated with resistance to CHG and mupirocin, and biofilm formation was found to contribute to this biocide resistance.
INFECTION AND DRUG RESISTANCE
(2022)