Article
Multidisciplinary Sciences
Efe Sezgin, Gamze Terlemez, Berkay Bozkurt, Goksel Bengi, Hale Akpinar, Ilker Buyuktorun
Summary: This study aims to investigate whether qPCR analysis of common candidate bacteria from a patient's fecal microbiome can serve as a fast and reliable personalized microbial biomarker for monitoring the disease course of inflammatory bowel diseases (IBD). The results showed that the relative abundance of anti-inflammatory and beneficial bacteria, such as Faecalibacterium prausnitzii, Akkermansia muciniphila, and Streptococcus thermophilus, was significantly lower in IBD patients compared to the control group. The relative abundance of these common species was also correlated with the severity of IBD. The qPCR analysis demonstrated lower variance and higher statistical power compared to NGS data, suggesting that it can be a powerful and cost-effective approach for monitoring disease status and identifying personalized treatment options for IBD patients.
Review
Chemistry, Medicinal
Yijie Song, Man Yuan, Yu Xu, Hongxi Xu
Summary: Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders caused by abnormal immune response. The development of proinflammatory cytokine therapeutic agents, including TNF inhibitors, shows promise for treating IBD. Antagonizing other cytokines has shown advantages in clinical trials and can be used to target anti-TNF therapy failure. Additionally, blocking lymphocyte homing helps alleviate inflammation and tissue damage.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Cell Biology
Bartolo Tamburini, Marco Pio La Manna, Lidia La Barbera, Leila Mohammadnezhad, Giusto Davide Badami, Mojtaba Shekarkar Azgomi, Francesco Dieli, Nadia Caccamo
Summary: Inflammatory bowel disease (IBD) is a pressing global health problem with increasing incidence worldwide. While the exact etiology is still unclear, a complex interaction of factors, including immune system defects, microbial dysbiosis, genetic predisposition, and environmental factors, contribute to the development of inflammation. This review aims to analyze the intricate balance between these elements in maintaining intestinal homeostasis and preventing or minimizing the adverse effects of IBD on health.
Article
Medicine, General & Internal
Burcu Ozdemir, Ali Atay, Meral Akdogan Kayhan, Yasemin Ozderin Ozin, Dilara Turan Gokce, Adalet Altunsoy, Rahmet Guner
Summary: The study found that tissue CMV-DNA PCR testing is more useful for diagnosing CMV colitis and enables rapid and appropriate treatment.
Article
Oncology
Aileen I. Fernandez, Niki Gavrielatou, Leena McCann, Saba Shafi, Myrto K. Moutafi, Sandra Martinez-Morilla, Ioannis A. Vathiotis, Thazin Nwe Aung, Vesal Yaghoobi, Yalai Bai, Yvonne G. Chan, Jodi Weidler, Roy Herbst, Michael Bates, David L. Rimm
Summary: This study investigates the use of PD-L1 mRNA level as a predictive biomarker for nonresponse to immune checkpoint inhibitors (ICIs) in lung cancer patients. The results show that high levels of PD-L1 and PD-L2 mRNA are associated with improved long-term benefit and overall survival, while low PD-L1 mRNA level has a high negative predictive value for absence of long-term benefit.
JOURNAL OF THORACIC ONCOLOGY
(2022)
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Salvatori Silvia, Marafini Irene, Venuto Chiara, Laudisi Federica, Neri Benedetto, Lavigna Diletta, Franchin Martina, De Cristofaro Elena, Biancone Livia, Calabrese Emma, Giannarelli Diana, Giovanni Monteleone
Summary: Frailty is common in hospitalized patients with inflammatory bowel disease (IBD) and has been associated with an increased risk of adverse outcomes. This prospective study revealed that nearly one-fifth of IBD patients were frail, with active disease being an independent risk factor for the frail phenotype.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Biochemical Research Methods
John S. Cognetti, Maya T. Moen, Matthew G. Brewer, Michael R. Bryan, Joshua D. Tice, James L. McGrath, Benjamin L. Miller
Summary: Tissue chip devices aim to simulate human physiology on a small scale and improve upon animal models. By incorporating photonic biosensors, this research enables real-time sensing of specific biomolecules in a model tissue chip device, which has applications in drug development and disease modeling.
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Gastroenterology & Hepatology
Padhmanand Sudhakar, Judith Wellens, Bram Verstockt, Marc Ferrante, Joao Sabino, Severine Vermeire
Summary: Inflammatory bowel disease (IBD) is a global disease that affects the gastrointestinal tract and also has an impact on the mental and emotional well-being of patients. Despite medical advancements, only a third of diagnosed patients achieve short to medium-term remission. Therefore, some patients might resort to complementary and alternative approaches to manage their symptoms. This study reviews the efficacy of these approaches and proposes a patient-centric clinical care model and recommendations.
Article
Gastroenterology & Hepatology
Giorgos Bamias, Theresa T. Pizarro, Fabio Cominelli
Summary: This review describes the cellular and molecular immunological mechanisms of intestinal fibrosis in IBD, with a particular focus on animal models of intestinal fibrosis. Based on this research, potential future targets for antifibrotic therapies for IBD patients can be identified.
INFLAMMATORY BOWEL DISEASES
(2022)
Review
Nutrition & Dietetics
Michal Sienkiewicz, Patrycja Szymanska, Jakub Fichna
Summary: Inflammatory bowel disease is a group of chronic relapsing disorders with insufficient available therapeutic approaches; current treatment strategies target immune system dysfunctions, with auxiliary therapies involving personalized diet and healthy lifestyle.
ADVANCES IN NUTRITION
(2021)
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Gastroenterology & Hepatology
Joseph Diab, Rania Al-Mahdi, Sandra Gouveia-Figueira, Terkel Hansen, Einar Jensen, Rasmus Goll, Thomas Moritz, Jon Florholmen, Guro Forsdahl
INFLAMMATORY BOWEL DISEASES
(2019)
Article
Infectious Diseases
H. Kileng, T. Gutteberg, R. Goll, E. J. Paulssen
BMC INFECTIOUS DISEASES
(2019)
Article
Biochemical Research Methods
Armin Schniers, Rasmus Goll, Yvonne Pasing, Sveinung Wergeland Sorbye, Jon Florholmen, Terkel Hansen
CLINICAL PROTEOMICS
(2019)
Article
Gastroenterology & Hepatology
Joseph Diab, Terkel Hansen, Rasmus Goll, Hans Stenlund, Maria Ahnlund, Einar Jensen, Thomas Moritz, Jon Florholmen, Guro Forsdahl
INFLAMMATORY BOWEL DISEASES
(2019)
Article
Gastroenterology & Hepatology
Midori Kjellin, Hege Kileng, Dario Akaberi, Navaneethan Palanisamy, Ann-Sofi Duberg, Astrid Danielsson, Magnhild Gangsoy Kristiansen, Johan Nojd, Soo Aleman, Tore Gutteberg, Rasmus Goll, Anders Lannergard, Johan Lennerstrand
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2019)
Article
Gastroenterology & Hepatology
Mona Dixon Gundersen, Rasmus Goll, Christopher Graham Fenton, Endre Anderssen, Sveinung Wergeland Sorbye, Jon Ragnar Florholmen, Ruth Hracky Paulssen
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Joseph Diab, Terkel Hansen, Rasmus Goll, Hans Stenlund, Einar Jensen, Thomas Moritz, Jon Florholmen, Guro Forsdahl
Article
Gastroenterology & Hepatology
Christian Borde Arkteg, Rasmus Goll, Mona Dixon Gundersen, Endre Anderssen, Christopher Fenton, Jon Florholmen
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2020)
Article
Medicine, General & Internal
Peter Holger Johnsen, Frank Hilpusch, Per Christian Valle, Rasmus Goll
Article
Gastroenterology & Hepatology
Leif Kyrre Berg, Rasmus Goll, Erik Fagerli, Judith Krey Ludviksen, Hilde Fure, Odd Sverre Moen, Sveinung W. Sorbye, Tom Eirik Mollnes, Jon Florholmen
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2020)
Article
Multidisciplinary Sciences
Guanglin Cui, Aping Yuan, Zhenfeng Li, Rasmus Goll, Jon Florholmen
SCIENTIFIC REPORTS
(2020)
Article
Gastroenterology & Hepatology
Rasmus Goll, Peter Holger Johnsen, Erik Hjerde, Joseph Diab, Per Christian Valle, Frank Hilpusch, Jorunn Pauline Cavanagh
Article
Multidisciplinary Sciences
Christian Borde Arkteg, Sveinung Wergeland Sorbye, Lene Buhl Riis, Stig Manfred Dalen, Jon Florholmen, Rasmus Goll
Summary: Despite being in clinical and endoscopic remission, 7-35% of UC patients show histological inflammation on a level classified as active disease, depending on the index and cut-off used for evaluation. The ability to accurately and consistently classify these patients could be improved.
Article
Multidisciplinary Sciences
Oddmund Nestegard, Behrouz Moayeri, Fred-Arne Halvorsen, Tor Tonnesen, Sveinung Wergeland Sorbye, Eyvind Paulssen, Kay-Martin Johnsen, Rasmus Goll, Jon Ragnar Florholmen, Kjetil K. Melby
Summary: This study characterized the antimicrobial resistance pattern of H. pylori before and after treatment, and found that clarithromycin resistance was more frequent in the treatment-resistant group. No resistance was observed for amoxicillin and levofloxacin.
Article
Endocrinology & Metabolism
M. A. Larsen, V. T. Isaksen, E. J. Paulssen, R. Goll, J. R. Florholmen