Journal
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
Volume 60, Issue 3, Pages 367-374Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S1021443713020040
Keywords
Solanum tuberosum; transgenic plants; Delta 12-acyl-lipid desaturase; polyunsaturated fatty acids; low temperatures; free-radical processes; CO2 exchange; antioxidant enzymes
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Funding
- Russian Foundation for Basic Research [11-04-00719a]
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The effects of potato (Solanum tuberosum L., cv. Desnitsa) plant transformation with the desA gene encoding Delta 12-acyl-lipid desaturase from Synechocystis sp. PCC 6803 on the regulation of free-radical processes in relation to plant tolerance to hypothermia are considered. It was shown that the content of polyunsaturated fatty acids (PUFA) in transformed plants was higher than in wild-type ones. In particular, the content of linoleic acid in transformants was higher by 35% and the content of linolenic acid was by 41% higher than in untransformed plants. In addition, transformation induced an increase in the absolute content of C-16-PUFA and on the whole resulted in a marked accumulation of membrane lipids. As judged from the values of the damage index and the ratio of photosynthesis to respiration in wild-type and transformed plants under cold treatment, these changes in lipid metabolism favored the protection of coupling membranes, thus preventing plants against free-radical oxidation under low-temperature stress. As a result, the intensity of oxidative stress in transformed plants was much lower than in wild-type ones, whereas antioxidant enzymes (superoxide dismutase, catalase, peroxidase) were not substantially activated under hypothermia.
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