Journal
RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY B
Volume 5, Issue 2, Pages 320-325Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1990793111020400
Keywords
human serum albumin; denaturation; fluorescence; probe; eosin
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Funding
- Russian Foundation for Basic Research [07-02-00459]
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An analysis of the intrinsic fluorescence of the protein and the fluorescence of the eosin molecular probe in solutions of the protein was used to study the denaturation of human serum albumin (HSA) under the action of cetyltrimethylammonium bromide (CTAB), a cationic detergent, at various pH values. The denaturation of HSA under the influence of CTAB is a single-stage process over the entire pH range covered, pH (3.5-8.0). The maximum possible loosening of HSA globules in the presence of CTAB is reached at [CTAB] = 4 mmol/l. This detergent effectively denatures HSA at pH values higher than the pI of the protein (4.7).
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