Article
Spectroscopy
Zsolt Preisz, Sandor Kunsagi-Mate
Summary: The study found that both methotrexate and its photodegradation products bind to human serum albumin through entropy-driven interactions, leading to increased stability of the albumin. Additionally, the presence of methotrexate increases the activation energy, which may contribute to fine tuning methotrexate applications in therapy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Applied
Dimuthu Bogahawaththa, Todor Vasiljevic
Summary: Shearing can alter the secondary structure and denaturation extent of proteins, with different effects observed for IgG and BSA. Shearing at specific temperatures may have a countereffect against thermal denaturation, while in most conditions, shearing induced denaturation of proteins.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Physical
Md Iqbal Hossain, G. J. Blanchard
Summary: Room temperature ionic liquids (RTILs) have unique properties, including the ability to support induced free charge density gradient. When diluted, RTILs form micelle-like structures and show compositional non-uniformity. In this study, we investigated the dilution of two imidazolium RTILs with polar aprotic solvents and found that the properties depend on the RTIL anion. Evidence of aggregated domains in the non-affected regions of RTILs was observed, and the domain size increased with increasing dilution.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Jimin Park, Mi-Sun Kim, Taeseong Park, Young Hwan Kim, Dong Hae Shin
Summary: Pharmaceutical-grade HSA has altered oxidative and ligand-binding properties compared to native HSA, which provides important insights for the production of p-HSA solutions and HSA-based biomaterials with higher biorelevance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Shizhen Meng, Qingqing Yu, Ming Li, Xin Liu, Xiaole Zhao, Kejia Wu, Qiao Wang, Yan Liu, Yongning Wu, Zhiyong Gong
Summary: This study focuses on the binding interactions between alkyl imidazolium-based ionic liquids (ILs) and human serum albumin (HSA). The ILs were found to primarily bind to the hydrophobic cavity of HSA through hydrogen bonding and van der Waals forces, forming stable complexes via static quenching. The binding of ILs affected the secondary structure of HSA and may influence their bioavailability and distribution.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Danuta Kruk, Elzbieta Masiewicz, Sylwia Lotarska, Roksana Markiewicz, Stefan Jurga
Summary: H-1 spin-lattice relaxation experiments were conducted on a series of ionic liquids containing different alkyl chain lengths of bis(trifluoromethanesulfonyl)imide anion and cations. The results provided insights into the rotational and translational dynamics of the cations in comparison to molecular liquids, indicating a temperature-dependent ratio between the correlation times of these motions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Ziqiao Yan, Minyi He, Yuwei Zhang, Gengxin Hu, Hong Li
Summary: The study demonstrates that methylene blue can significantly amplify the oxidation signals of tyrosine residues in proteins. By evaluating various factors, the mechanism of signal amplification is determined. This method of signal enhancement shows good selectivity, high reproducibility, and strong anti-interference ability.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Instruments & Instrumentation
Cecilia Spedalieri, Julian Plaickner, Eugen Speiser, Norbert Esser, Janina Kneipp
Summary: The UVRR spectra of BSA and HSA proteins in aqueous solution were compared to distinguish between them despite their similar amino acid composition and structure. Tryptophan residues were detected despite their low abundance, and the dominant contribution from tryptophan, tyrosine, and phenylalanine was observed in the spectra. In the high-frequency region, the signals from overtones and combination bands of phenylalanine and tyrosine were clearly observed.
APPLIED SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Francis H. C. Tsao, Keith C. Meyer
Summary: The age-dependent conformational stability of human serum albumin was evaluated using the fluorescent bilayer liposome assay. It was found that albumin in the sera of older individuals exhibited hydrophobic effects on liposomes and made liposomal membrane more prone to hydrolysis, while albumin in the sera of younger individuals remained stable. These findings suggest that age-related changes in albumin stability may impact its normal functions and membrane integrity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Ana M. Ferreira, Maria V. Quental, Mukesh Sharma, Dibyendu Mondal, Augusto Q. Pedro, Joao A. P. Coutinho, Mara G. Freire
Summary: Early infection diagnosis is essential for reducing morbidity and mortality rates. A study investigated the use of aqueous biphasic systems (ABS) composed of polypropylene glycol 400 and cholinium-based ionic liquids (ILs) at different pH values for pre-treating human serum, aiming to separate DNA from human serum albumin (HSA) to improve the accuracy of DNA quantification. The ABS demonstrated high extraction efficiencies of DNA to the IL-rich phase, with minimal DNA loss. It was found that adjusting the pH allowed for selective isolation of DNA from HSA. The IL-rich phase of the ABS was shown to maintain DNA integrity and could potentially serve as a DNA storage medium.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Physical
Elham Ziaee, Behzad Shareghi, Sadegh Farhadian
Summary: BSA, as a carrier protein, plays important roles in maintaining extracellular fluid volume stability and interacting with polyamines such as spermine. The binding of spermine to BSA alters its structure and significantly affects its fluorescence properties, indicating a stabilizing effect of spermine on BSA.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Eszter Fliszar-Nyul, Zelma Faisal, Renata Skaper, Beata Lemli, Bayarsaikhan Bayartsetseg, Csaba Hetenyi, Patrik Gombos, Andras Szabo, Miklos Poor
Summary: Beauvericin, cyclopiazonic acid, and sterigmatocystin are emerging mycotoxins that can contaminate food and animal feed, resulting in economic losses and health risks. Human serum albumin can form stable complexes with these mycotoxins, influencing their toxicokinetics and potentially serving as a cost-effective affinity protein. This study investigated the interactions of these mycotoxins with human serum albumin using various techniques, revealing the formation of low to moderately strong complexes. The interactions of cyclopiazonic acid with site markers suggest potential toxicological importance.
Article
Biochemistry & Molecular Biology
Alexey Chubarov, Anna Spitsyna, Olesya Krumkacheva, Dmitry Mitin, Daniil Suvorov, Victor Tormyshev, Matvey Fedin, Michael K. Bowman, Elena Bagryanskaya
Summary: The Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to study reversible non-covalent dimers of Human Serum Albumin (HSA), showing that chemical modifications of Cys-34 can affect dimerization. The weak non-covalent dimers formed by HSA at physiological concentrations have a well-defined structure and can easily revert back to monomers, playing an important role in transport and binding processes.
Article
Chemistry, Multidisciplinary
Dorota Kowalska, Stefan Stolte, Dariusz Wyrzykowski, Piotr Stepnowski, Joanna Dolzonek
Summary: The bioaccumulation potential of ionic liquids (ILs) is often overlooked, despite their confirmed environmental persistence and toxicity risk. Studies have shown that ILs can strongly bind to human serum albumin (HSA), indicating a potential role of blood proteins in bioaccumulation. Additionally, the interaction between IL cations and anions may also affect their affinity to HSA.
Review
Pharmacology & Pharmacy
Aziz Ullah, Goeun Shin, Sung In Lim
Summary: This review evaluates the current state of knowledge regarding various aspects of ABMs and the unique advantages of ABM-mediated drug delivery. It also discusses how ABMs can be specifically modulated to maximize potential benefits in clinical development.
DRUG DISCOVERY TODAY
(2023)