Article
Chemistry, Analytical
James Otis, Joerg Hofmann, Thomas Schmidt, Christoph Buttersack, Roger Glaeser
Summary: In this study, Y-zeolite was used as the stationary phase in HPLC column, and methanol-water mixture was used as the eluent. The results showed that Y-zeolite exhibited excellent separation performance and could effectively separate isomers of polyols and monosaccharides. Compared to traditional hydrophilic interaction chromatography (HILIC), Y-zeolite showed superior selectivity and separation efficiency.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Kenichi Nagase, Yuta Ishizawa, Masakazu Inoue, Matsurika Kokubun, Sota Yamada, Hideko Kanazawa
Summary: The temperature-responsive spin column presents a simple sample preparation method using all-aqueous eluent for protein removal from serum, required for serum drug analysis. Two types of silica beads grafted with temperature-responsive copolymers were utilized as packing materials, enabling effective separation of serum proteins and analytes by changing the temperature during elution.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Beata Polak, Emilia Pajurek
Summary: The research focused on separating water- and fat-soluble vitamins using micellar systems of HPTLC and PPEC, investigating the influence of mobile phase composition on migration distances and zone separation. Comparisons between HPLC and PPEC showed differences in system capacity, with PPEC performing better for fat-soluble vitamins and exhibiting lower variances and reproducibilities.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Jonas Neumann, Sebastian Schmidtsdorff, Alexander H. Schmidt, Maria K. Parr
Summary: Currently, there is limited information on the use of ternary eluent compositions in supercritical fluid chromatography for peptide separation. This study investigates the benefits of adding acetonitrile to methanol as a modifier, increasing the resolution of peptide fingerprints and altering the elution order of analytes. A generic method development protocol for polypeptides is proposed based on the optimized conditions for three cyclic antibiotic peptides.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Bassam Lajin, Walter Goessler
Summary: The importance of element-selective detection with ICPMS has been enhanced by the introduction of tandem ICPMS/MS, enabling nonmetal speciation analysis. This study reports the first phosphorous speciation study in human urine using HPLC-ICPMS/MS, involving the determination of phosphoethanolamine. A simple derivatization procedure was employed for separation, and a novel chromatographic eluent, hexanediol, was used to address the challenge of eluting the hydrophobic derivative. The developed method showed fast chromatographic separation, no need for an isotopically labeled internal standard, and high accuracy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Organic
Yasumasa Fukushima, Shunichi Aikawa
Summary: The complex of alizarin complexone (ALC) and Ni2+ acts as a colorimetric probe for selectively recognizing histamine in aqueous solution. The color changes from red to purple, and histamine can be detected in a linear range of 5-150 μM with a detection limit of 7.68 μM.
TETRAHEDRON LETTERS
(2021)
Article
Biochemical Research Methods
Heidi Ostby, John-Kristian Jameson, Thales Costa, Vincent G. H. Eijsink, Magnus O. Arntzen
Summary: Research on oligosaccharides, including the product mixtures generated by LPMOs, is growing rapidly. A novel method based on dual electrolytic eluent generation has been developed to separate and quantify these compounds with high sensitivity and improved analytical precision. This new chromatographic platform offers simplified operation and rapid method optimization, overcoming drawbacks associated with manual eluent preparation.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Bassam Lajin, Walter Goessler
Summary: The use of DMC in ICPMS facilitates elution and detection of novel hydrophobic compounds, improves column recovery, without compromising detection limits.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Satoki Okabayashi, Leona Kawane, Nanda Yusentri Mrabawani, Takahiro Iwai, Tomohiro Narukawa, Motohiro Tsuboi, Koichi Chiba
Summary: A novel technique using HILIC combined with ICP-MS was developed to identify and quantify Gd-based contrast agents in river water. The study found both known Gd-based contrast agents and unidentified Gd compounds in river water samples, suggesting potential transformation or dissociation of Gd chelates during treatment in WWTP.
Article
Biochemistry & Molecular Biology
Niels A. W. de Kok, Marten Exterkate, Ruben L. H. Andringa, Adriaan J. Minnaard, Arnold J. M. Driessen
Summary: A versatile RP-UHPLC-MS method using 1-butanol as the eluent is presented for separating highly hydrophobic lipids, demonstrated through analysis of lipid extracts of Escherichia coli and Sulfolobus acidocaldarius, offering enhanced separation of cardiolipins and extending the analytical range for RPLC.
CHEMISTRY AND PHYSICS OF LIPIDS
(2021)
Article
Biochemical Research Methods
Qing Fu, Huanhuan Zhang, Zhuoshun Dai, Dasen Jiang, Meng Sun, Yanxiong Ke, Yu Jin, Xinmiao Liang
Summary: An effective strategy of using acetonitrile-methanol-water as mobile phase was developed to achieve acceptable peak shape of steviol glycosides in reversed-phase liquid chromatography (RPLC). It was found that the elution profile of RA is highly dependent on the ternary composition, and with the increase of acetonitrile concentration in the ternary eluent, the peak shape of RA changes from tailing to fronting shapes. The use of ternary eluent can control and adjust the peak shape of steviol glycosides in RPLC separation.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Chemistry, Physical
Wojciech Gluszewski, Natalia Okroj
Summary: The study discusses the impact of gamma radiation on the decomposition of 90% and 98% hydrogen peroxide, as well as its potential use as a rocket propellant. Results obtained through gravimetric and gas chromatography methods are compared, showing the relationship between dose size and post-radiation oxygen release. Gas chromatography with a packed column and a thermal conductivity detector is identified as a convenient method for evaluating the stability of HTP-based rocket propellant.
RADIATION PHYSICS AND CHEMISTRY
(2021)
Article
Chemistry, Physical
Daniel Adjei, Sergey A. Denisov, Mehran Mostafavi
Summary: This study investigated the dynamics of radiolytic species induced in fused silica by picosecond electron pulses. The results identified at least two main species induced in the material, one with strong absorption in the UV range and the other with maximum absorption at 580 nm. Additionally, low absorbance was observed in the NIR range, decaying within 11 ps after the pulse.
RADIATION PHYSICS AND CHEMISTRY
(2022)
Article
Automation & Control Systems
Xiaogang Wang, Yongjun Shi, Yankuo Guo, Xianfa Li, Xiaoyu Zhao
Summary: Laser forming is a non-contact forming technology that uses uneven thermal stress induced by a laser light source to deform plates. The study achieved saddle surface and bowl shape surface by using a round 7075 aviation aluminum plate and investigated the deformation rule under various technology parameters. Results show that the accuracy of the forming surface is affected by the non-uniform distribution of temperature on the heating line.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2021)
Article
Cell Biology
Daniel Pena-Oyarzun, Marcelo Rodriguez-Pena, Francesca Burgos-Bravo, Angelo Vergara, Catalina Kretschmar, Cristian Sotomayor-Flores, Cesar A. Ramirez-Sarmiento, Humbert De Smedt, Montserrat Reyes, William Perez, Vicente A. Torres, Eugenia Morselli, Francisco Altamirano, Christian A. M. Wilson, Joseph A. Hill, Sergio Lavandero, Alfredo Criollo
Summary: The formation of a protein complex between PKD2 and BECN1 is essential for the induction of autophagy, and this process relies on the presence of the CC1 domain of PKD2 and on the mobilization of intracellular Ca(2+) by PKD2.
