Journal
RHEUMATOLOGY
Volume 51, Issue 6, Pages 987-991Publisher
OXFORD UNIV PRESS
DOI: 10.1093/rheumatology/ker430
Keywords
maternal microchimersim; juvenile dermatomyositis; autoimmunity
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Funding
- Wellcome Trust UK
- Action Medical Research UK
- Henry Smith Charity
- North Bristol Trust
- Diabetes UK
- Juvenile Diabetes Research Foundation
- European Foundation for the Study of Diabetes
- Diabetes UK [07/0003562] Funding Source: researchfish
- Versus Arthritis [18474] Funding Source: researchfish
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Objective. Recent advances in molecular techniques have revealed that there is bi-directional transfer of cells between mother and child during pregnancy, and the presence of a mother's cells in her child has been termed maternal microchimerism (MMc). There is the potential for maternal cells to provoke inappropriate immune responses in the child, which could be a factor in autoimmunity including JDM. The aim of this study was to determine whether maternal (female) cells could be detected in frozen muscle sections from seven males (age range 3-13 years) with JDM participating in the Juvenile Dermatomyositis National (UK and Ireland) Cohort Biomarker Study and Repository for Idiopathic Inflammatory Myopathies and sections of muscle controls (age range 2-12 years). Methods. At least 1000 cells from each section underwent FISH and confocal imaging through each nucleus. Concomitant IF for CD45 was used to determine whether MMc in muscle were lymphocytes. A non-parametric Mann-Whitney U-test was used to detect statistical differences. Results. The frequency of MMc was higher in JDM muscle (0.42-1.14%) than in controls (0.08-0.42%) P = 0.01. No CD45+ MMc were observed. Conclusion. These data confirm an increased frequency of MMc in JDM. More detailed characterization of MMc is required, particularly using phenotypic markers, to explain the role of these cells in JDM.
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