Article
Immunology
Qingfeng Wang, Shuang Wu, Xingsheng Ye, Shiyao Tan, Fanfan Huang, Guannan Su, Aize Kijlstra, Peizeng Yang
Summary: Public metagenomic studies have revealed an association between the gut microbiome and immune-mediated diseases. This study integrated sequencing data from various uveitis entities and compared the gut microbiome signatures, revealing significant differences between different diseases and healthy controls.
JOURNAL OF AUTOIMMUNITY
(2023)
Review
Cell Biology
Fabian Vega-Tapia, Mario Bustamante, Rodrigo A. Valenzuela, Cristhian A. Urzua, Loreto Cuitino
Summary: miRNAs play a role in regulating gene expression in inflammatory and autoimmune diseases, and their dysregulation is associated with the development of Vogt-Koyanagi-Harada (VKH) disease, a chronic multisystem pathology with autoimmune etiology. The drugs commonly used in VKH treatment have significant influence on miRNA expression, suggesting that miRNAs could be potential therapeutic targets for developing more effective treatments for VKH.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Xiuxing Liu, Qi Jiang, Jianjie Lv, Shizhao Yang, Zhaohao Huang, Runping Duan, Tianyu Tao, Zhaohuai Li, Rong Ju, Yingfeng Zheng, Wenru Su
Summary: In this study, we conducted a multimodal, high-dimensional immune atlas analysis using single-cell RNA sequencing and mass cytometry to investigate VKH patients undergoing TOFA therapy. The study revealed polarization of T cells, accumulation of monocytes, upregulation of cytokines and JAK/STAT signaling pathways in VKH patients. TOFA reversed the Th17/Treg imbalance, alleviated VKH symptoms, and reduced inflammation-related responses and intercellular interactions. Furthermore, the study identified two monocyte subpopulations strongly associated with VKH pathogenesis and mechanisms of TOFA treatment.
Article
Immunology
Jia Shu, Guannan Su, Jun Zhang, Zhangluxi Liu, Rui Chang, Qingfeng Wang, Peizeng Yang
Summary: Recent studies have uncovered the importance of circRNAs in various biological processes and autoimmune diseases, with a focus on their potential role in the classical autoimmune disease Vogt-Koyanagi-Harada (VKH) disease. Through RNA-seq analysis of VKH patients and healthy controls, differentially expressed circRNAs, miRNAs, and mRNAs were identified, suggesting a potential immunomodulatory function of circRNAs in the pathophysiology of VKH disease. Functional enrichment analyses indicated involvement of inflammatory response, adaptive immune response, and various signaling pathways in VKH disease pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Begona De Domingo, Miguel Lopez, Maria Lopez-Valladares, Esperanza Ortegon-Aguilar, Bernardo Sopena-Perez-Arguelles, Francisco Gonzalez
Summary: We present a case of exacerbation of Vogt-Koyanagi-Harada (VKH) disease following COVID-19 vaccination. A 46-year-old woman developed bilateral granulomatous uveitis after the first dose of COVID-19 mRNA vaccine (Comirnaty, Pfizer-BioNTech), and was diagnosed with complete VKH disease four days after receiving the second dose. The symptoms were successfully treated with high dose intravenous corticosteroids, followed by oral prednisone. The close temporal relationship between COVID-19 vaccination and exacerbation of VKH symptoms strongly suggests a causal link.
Article
Immunology
Han Jiang, Zhaohui Li, Long Yu, Ying Zhang, Li Zhou, Jianhua Wu, Jing Yuan, Mengyao Han, Tao Xu, Junwen He, Shan Wang, Chengfeng Yu, Sha Pan, Min Wu, Hangyu Liu, Haihong Zeng, Zhu Song, Qiangqiang Wang, Shen Qu, Junwei Zhang, Yafei Huang, Junyan Han
Summary: The study revealed that VKH patients have abnormal activation and differentiation of peripheral lymphocytes, and GC therapy with methylprednisolone (MP) can suppress the pathogenic immune response by directly inhibiting T cell activation and affecting monocyte subsets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Ninan Jacob, Mudit Tyagi, Jay Chhablani, Raja Narayanan, Anup Kelgaonkar, Mukesh Jain, Sumit Randhir Singh, Niroj Kumar Sahoo
Summary: This paper evaluates the role of retinal pigment epithelium (RPE) as a biomarker of inflammation in acute Vogt-Koyanagi-Harada (VKH) disease. The study found a significant decrease in RPE thickness and a significant increase in RPE reflectivity following resolution of the disease. Furthermore, there was a significant correlation between RPE and choroidal thickness during the acute stage of the disease.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Shizhao Yang, Tianyu Tao, Zhaohao Huang, Xiuxing Liu, He Li, Lihui Xie, Feng Wen, Wei Chi, Wenru Su
Summary: The study found that adalimumab was an effective and safe option for sight-threatening Vogt-Koyanagi-Harada (VKH) patients refractory to conventional therapy. It controlled inflammation, preserved visual function, and reduced the use of glucocorticoids.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Wanyun Zhang, Zhijun Chen, Kun Yi, Guannan Su, Yaning Liu, Yang Deng, Yinan Zhang, Qingfeng Cao, Yanlin Pu, Xiang Luo, Yujie Lai, Peizeng Yang
Summary: In this study, the researchers discovered that DNA 5-hmC and the TET2 gene play an important role in the development of Vogt-Koyanagi-Harada (VKH) disease. They found that the levels of DNA 5-hmC and TET activity were elevated in CD4+ T cells from active VKH patients, and identified 6 candidate target genes involved in the development of VKH disease. Functional experiments showed that TET2 up-regulated the expression of LRRC39, which in turn influenced Th1/Treg responses in CD4+ T cells. This study provides new insights into the pathogenesis of VKH and a potential target for epigenetic therapy.
CLINICAL IMMUNOLOGY
(2023)
Review
Medicine, General & Internal
Priscilla Manni, Maria Carmela Saturno, Massimo Accorinti
Summary: Vogt-Koyanagi-Harada (VKH) is a rare multisystem inflammatory disease that can be triggered by COVID-19 infection or vaccination. VKH cases following COVID-19 infection and vaccination have been reported, and the patients generally respond well to corticosteroid therapy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Guannan Su, Liping Du, Hongsong Yu, Minghui Li, Ruocheng Huang, Xiaonan Yang, Detao Wang, Qing Wang, Peizeng Yang
Summary: We conducted a genome-wide methylation association analysis of VKH disease by studying 60 VKH patients and 60 healthy controls. We identified aberrant CpG sites in both HLA and non-HLA gene regions that were associated with VKH disease. The mRNA levels of certain genes were found to be increased in VKH patients, consistent with the hypomethylated CpG status in those gene regions. Additionally, seven aberrantly methylated CpG sites were identified as potential diagnostic markers for VKH disease.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Immunology
Rui Chang, Yan Ji, Jing Xu, Yuxian Lai, Hang Zhang, Zhenyu Zhong, Guannan Su, Peizeng Yang
Summary: In this study, it was found that downregulation of Fc epsilon receptor Ig (FCER1G) due to DNA hypermethylation is responsible for resistance to the combination regimen of corticosteroids and cyclosporin A (CsA) in female patients. However, chlorambucil (CLB) reverses this resistance by inducing FCER1G expression via DNA demethylation. Modulation of FCER1G may be a promising sensitization strategy in female patients with resistance to CsA and corticosteroids.
CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Wanqian Li, Jun Tan, Siyuan He, Yingying Yue, Huan Liu, Ruonan Li, Xiaotang Wang, Guoqing Wang, Wei Fan, Chenyang Zhao, Qian Zhou, Peizeng Yang, Shengping Hou
Summary: Vogt-Koyanagi-Harada (VKH) disease is a blinding eye disease characterized by an autoimmune response against melanocytes in multiple organs. The role of retinal pigment epithelium (RPE) in the disease is crucial, but not well understood. In this study, a hiPSC RPE model of VKH disease was established, and patient-derived RPE cells showed similar characteristics to VKH disease, including barrier disruption and impaired phagocytosis. A small molecular compound targeting EGR2 was found to rescue RPE cell functions, suggesting a potential treatment strategy for VKH disease.
CLINICAL IMMUNOLOGY
(2023)
Article
Cell Biology
Rui Chang, Ying Zhu, Jing Xu, Lin Chen, Guannan Su, Aize Kijlstra, Peizeng Yang
Summary: The diagnosis of Vogt-Koyanagi-Harada (VKH) disease relies on complex clinical manifestations with lack of objective laboratory biomarkers. This study identified differential metabolites in urine of VKH patients compared to healthy controls, and between active VKH and inactive VKH patients. The abnormal metabolites could potentially serve as diagnostic and disease activity markers for VKH, but further confirmation is needed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kailei Guo, Baiyi Li, Fuhua Yang, Mi Zhang, Guixia Zhao, Xiaomin Zhang
Summary: Using miRNA sequencing, differential miRNA profiles between initial-onset and recurrent VKH patients were identified as potential biomarkers for understanding the pathogenesis of VKH disease. Pathway enrichment analysis revealed significant enrichment of target genes in the mitogen-activated protein kinase, tumor necrosis factor, and mechanistic target of rapamycin kinase pathways in the recurrent stage and shared DEMs. Additionally, the competing endogenous RNA network of hsa-miR-206 provides new insights for VKH treatment.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Clinical Neurology
Chaokui Wang, Wenjun Zhou, Guannan Su, Jianping Hu, Peizeng Yang
Summary: In uveitis and experimental autoimmune encephalomyelitis (EAE), PGRN plays an important role in immune regulation. These studies suggest that PGRN could serve as an immunoregulatory target for the prevention and treatment of Th1/Th17-mediated diseases.
NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION
(2022)
Article
Immunology
Wanqian Li, Jun Tan, Siyuan He, Yingying Yue, Huan Liu, Ruonan Li, Xiaotang Wang, Guoqing Wang, Wei Fan, Chenyang Zhao, Qian Zhou, Peizeng Yang, Shengping Hou
Summary: Vogt-Koyanagi-Harada (VKH) disease is a blinding eye disease characterized by an autoimmune response against melanocytes in multiple organs. The role of retinal pigment epithelium (RPE) in the disease is crucial, but not well understood. In this study, a hiPSC RPE model of VKH disease was established, and patient-derived RPE cells showed similar characteristics to VKH disease, including barrier disruption and impaired phagocytosis. A small molecular compound targeting EGR2 was found to rescue RPE cell functions, suggesting a potential treatment strategy for VKH disease.
CLINICAL IMMUNOLOGY
(2023)
Article
Ophthalmology
Ao Lu, Zhangluxi Liu, Guannan Su, Peizeng Yang
Summary: This cross-sectional bibliometric analysis provides an overview of global uveitis research in the last decade, including countries/regions, organizations, scholars, journals, trending topics, and fundings. The USA is the leading country in uveitis study, while Asian countries/regions like China and India play a substantial role. Trendy topics include biologics, COVID-19, and OCTA.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Ophthalmology
Xiaotang Wang, Qi Zhang, Shengping Hou, Jian Qi, Liping Du, Fuzhen Li, Qingfeng Cao, Peizeng Yang
Summary: The polymorphisms in PTPN6 and LncRNA C1RL-AS1 genes are associated with ocular BD in Han Chinese patients. The rs4013722 polymorphism of LncRNA C1RL-AS1 confers susceptibility to ocular BD, which is influenced by sex. The gene polymorphisms of PTPN6 are not associated with BD patients.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Ophthalmology
Suo Guo, Hui Liu, Yu Gao, Lingyu Dai, Jing Xu, Peizeng Yang
Summary: This study investigated vascular changes of fundus in quiescent Behcet uveitis (BU) using widefield swept source optical coherence tomography angiography and fluorescein angiography. The results showed that patients with BU had enlargement of foveal avascular zone and decreased vessel density within a 300 μm width around the foveal avascular zone in both superficial and deep retina. Furthermore, BU patients had lower vessel density of choriocapillaris in the peripheral fundus compared to controls. The combination of swept source optical coherence tomography angiography with fluorescein angiography accurately evaluated the changes in the retinal and choroidal vasculature in BU.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2023)
Article
Rheumatology
Jiayu Meng, Na Li, Xianyang Liu, Shengjun Qiao, Qian Zhou, Jun Tan, Ting Zhang, Zhifang Dong, Xiaopeng Qi, Aize Kijlstra, Liming Mao, Peizeng Yang, Shengping Hou
Summary: The study reveals that NLRP3 plays an anti-inflammatory role in experimental autoimmune encephalomyelitis and experimental autoimmune uveitis. It inhibits AIM2 inflammasome-mediated uveitis by regulating the p-SIK1/SREBF1 pathway.
ARTHRITIS & RHEUMATOLOGY
(2023)
Article
Immunology
Wanyun Zhang, Zhijun Chen, Kun Yi, Guannan Su, Yaning Liu, Yang Deng, Yinan Zhang, Qingfeng Cao, Yanlin Pu, Xiang Luo, Yujie Lai, Peizeng Yang
Summary: In this study, the researchers discovered that DNA 5-hmC and the TET2 gene play an important role in the development of Vogt-Koyanagi-Harada (VKH) disease. They found that the levels of DNA 5-hmC and TET activity were elevated in CD4+ T cells from active VKH patients, and identified 6 candidate target genes involved in the development of VKH disease. Functional experiments showed that TET2 up-regulated the expression of LRRC39, which in turn influenced Th1/Treg responses in CD4+ T cells. This study provides new insights into the pathogenesis of VKH and a potential target for epigenetic therapy.
CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Yunyun Zhu, Qiuyue Yu, Guannan Su, Na Shao, Jie Feng, Xiang Luo, Chunjiang Zhou, Peizeng Yang
Summary: This study investigated the mechanism of recombinant interferon-alpha 2a (IFN alpha 2a) in the treatment of Behcet's uveitis (BU) and found that IFN alpha 2a exerts its effects by modulating the function of dendritic cells (DCs) and CD4+ T cells.
CLINICAL IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xu Gao, Peizeng Yang, Cunliang Zhang, Deyang Kong, Yuefei Ji, Junhe Lu
Summary: This study reveals that nitrophenolic compounds can be formed by the reaction of natural organic matter and nitrite under sunlight. This suggests that the natural contribution of these compounds in the environment may have been overlooked.
ENVIRONMENTAL CHEMISTRY LETTERS
(2023)
Article
Ophthalmology
Jiankang Shan, Lin Li, Liping Du, Peizeng Yang
Summary: This study aimed to determine whether single nucleotide polymorphisms (SNPs) in the TBX21 gene are associated with susceptibility to acute anterior uveitis (AAU) in a Chinese population. The results showed no significant association between two candidate SNPs (rs4794067, rs11657479) in the TBX21 gene and AAU susceptibility. Stratification analysis and haplotype analysis also did not reveal any significant differences or associations. Therefore, the polymorphisms rs4794067 and rs11657479 in the TBX21 gene do not confer disease susceptibility to AAU in the Chinese population.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Ophthalmology
Peizeng Yang, Weiting Liao, Yanlin Pu, Zhenyu Zhong, Hongmiao Wang, Qiuyue Yu, Jinyu Cai, Wujiao Wang, Guannan Su
Summary: This study retrospectively analyzed the medical records of 2571 VKH patients and described the clinical features and visual prognosis among pediatric, adult, and elderly patients. The results showed that adult VKH patients have an increased risk of poor visual outcomes, possibly due to increased frequency of macular abnormalities.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Tingting Zhao, Qingfeng Cao, Chunjiang Zhou, Yao Wang, Liping Du, Peizeng Yang
Summary: This study found that the HLA-DRB1 * 04:05 and DQB1 * 04:01 alleles, as well as the DRB1 * 04:05-DQB1 * 04:01 haplotype, could be potential risk factors for SO.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Chemistry, Multidisciplinary
Ruonan Li, Jiangyi Liu, Ping Yi, Xianli Yang, Jun Chen, Chenyang Zhao, Xingyun Liao, Xiaotang Wang, Zongren Xu, Huiping Lu, Hongshun Li, Zhi Zhang, Xianyang Liu, Junjie Xiang, Ke Hu, Hongbo Qi, Jia Yu, Peizeng Yang, Shengping Hou
Summary: Through single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing, the heterogeneity of retinal progenitor cells and their differentiation into seven major types of retinal cells is explored. Lineage-determining transcription factors are identified and their regulatory networks at the transcriptomic and epigenomic levels are refined. Treatment with the inhibitor of RE1 silencing transcription factor, X5050, induces more neurogenesis and reduces Muller glial cells. The signatures of major retinal cells and their correlation with pathogenic genes associated with ocular diseases are described, providing an integrated framework for exploring the single-cell developmental dynamics of the human primary retina.
Article
Ophthalmology
Qingyan Jiang, Qingfeng Wang, Shiyao Tan, Jinyu Cai, Xingsheng Ye, Guannan Su, Peizeng Yang
Summary: This study explored the potential role of plasma-derived exosomal microRNAs (miRNAs) in the development of regulatory T cell (Treg)/T helper 17 (Th17) cell imbalances in Behcet's uveitis (BU). Exosomes from patients with active BU promoted Th17 cell differentiation and inhibited Treg cell differentiation. Highly expressed miR-19b-3p induced a Treg/Th17 cell imbalance, probably by downregulating CD46 expression, and its inhibition restored the Treg/Th17 cell balance in CD4+T cells from patients with active BU.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Genetics & Heredity
Chunya Zhou, Shiya Cai, Yuhong Xie, Zhen Zeng, Jun Zhang, Guannan Su, Qiuying Wu, Xingsheng Ye, Qingfeng Cao, Peizeng Yang, Jianmin Hu
Summary: This study revealed an association between PRKCD rs74437127 and CARD9 rs3812555 polymorphisms and VKH susceptibility, and showed that the increased susceptibility of rs3812555 for VKH may be mediated by regulating CARD9 gene expression and the production of pro-inflammatory cytokines, such as TNF-alpha.