Article
Physiology
Ana Caroline P. Gandara, Felipe A. Dias, Paula C. de Lemos, Renata Stiebler, Ana Cristina S. Bombaca, Rubem Menna-Barreto, Pedro L. Oliveira
Summary: Low levels of ROS play essential roles in cell signaling, with RpNOX5 and RpXDH regulating ROS in the digestive physiology of the kissing bug Rhodnius prolixus. Silencing these genes led to early mortality in adult females, impaired gut motility, decreased egg production, and disrupted hemoglobin digestion, indicating their crucial role in blood digestion control. Crosstalk between RpNOX5 and RpXDH influences redox metabolism and nitrogen excretion, highlighting their central role in digestive physiology.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kei Miyano, Mizuho Kajikawa
Summary: Six gene splice variants of Nox5 have been found in humans, and their N-terminal cytoplasmic domains differ in sequence and contain four EF-hand motifs. The association and dissociation between the N- and C-terminal cytoplasmic domains of the Nox5 beta variant are affected by alanine substitutions at the connecting loop between the third and fourth EF-hand motifs. These substitutions impair the cell surface localization of Nox5 beta. The Nox5 epsilon/S variant, lacking all EF-hand motifs, does not localize to the plasma membrane. Therefore, the Ca2+-sensitive intramolecular interaction determines the subcellular localization of Nox5 variants and their ability to generate superoxide.
Article
Andrology
Sara Keshtgar, Esmaeel Ghani
Summary: This study compares the production of reactive oxygen species (ROS) and sperm function in teratozoospermic and normozoospermic semen samples after calcium overload and NOX5 activation. The results suggest that teratozoospermic sperm are more vulnerable and produce more ROS compared to normozoospermic sperm.
Article
Medicine, Research & Experimental
Josiane Fernandes da Silva, Juliano Alves, Julio A. Silva-Neto, Rafael M. Costa, Karla B. Neves, Rheure Alves-Lopes, Livia L. Camargo, Francisco J. Rios, Augusto C. Montezano, Rhian M. Touyz, Rita C. Tostes
Summary: The study found that LPC induces oxidative stress in endothelial cells through NOX5-dependent ROS production, which is linked to increased intracellular calcium levels. Inhibition of NOX5 decreased LPC-induced ROS generation, as well as ICAM-1 expression and monocyte adhesion to endothelial cells. These results highlight the key role of NOX5 in early LPC-induced endothelial oxidative stress and inflammation, indicating its importance in atherosclerosis development.
Article
Biochemistry & Molecular Biology
Qiong Ding, Lin Luo, Lan Yu, Si-lu Huang, Xiao-qin Wang, Bo Zhang
Summary: Vitiligo is a depigmented disease with a simple diagnosis but difficult cure. Ermanin, a flavonoid extracted from bee glue used in traditional Chinese medicine, has been found to promote melanin production and regulate glutathione redox balance to assist in treating vitiligo.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
James Patrick Heiserman, Zenab Minhas, Elahe Nikpayam, Dong-Joo Cheon
Summary: Most ovarian cancer patients develop recurrent cancers which are often resistant to commonly employed chemotherapy agents, such as cisplatin. In this study, we investigated the role of heat shock protein 27 (HSP27) and fatty acid oxidation (FAO) in promoting cisplatin resistance. Our results showed that inhibiting HSP27 and FAO enhanced the cytotoxic effect of cisplatin and depleted cisplatin-induced ROS in cisplatin-resistant ovarian cancer cell lines. This dual inhibition strategy also increased cisplatin's cytotoxicity in in vivo xenograft tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alphonse Umugire, Yoon Seok Nam, Ye Eun Nam, Young Mi Choi, Se Myeong Choi, Sungsu Lee, Jong Hyun Cho, Hyong-Ho Cho
Summary: Cisplatin-induced ototoxicity, which is characterized by hearing impairment, may be caused by the production of reactive oxygen species (ROS) and DNA damage in cochlear hair cells (HC), but the exact mechanism is unknown. Avenanthramide-C (AVN-C), a natural antioxidant, was tested in normal adult mice to prevent cisplatin-induced hearing loss. In both in vivo and in vitro experiments, AVN-C showed a protective effect against cisplatin-induced damage by reducing ROS production, inflammatory cytokine expression, and DNA damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ye Ji Jeon, Sang Ah Yi, Jaecheol Lee, Jeung-Whan Han
Summary: S6K1 plays a crucial role in regulating cell proliferation and growth in the mTOR signaling pathway. Our study demonstrates the dynamic distribution of S6K1 in the cytoplasm and nuclei of human cervical cancer cells and reveals its additional role as a gene transcription regulator. These findings provide important insights into the mechanisms of tumorigenesis and potential therapeutic strategies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Liang Ding, Yalei Wen, Xin Zhang, Fang Zhao, Kenao Lv, Jian-hong Shi, Shigang Shen, Xuefeng Pan
Summary: The study found that CBP and Ku70 positively regulate the expression of Ku70, negatively regulate NOX2, and mitigate intracellular ROS in human melanoma. Knocking down CBP or Ku70 induced necrotic and paraptotic cell death, with chromosomal condensations related to the BAX-associated intrinsic pathway of apoptotic cell death. The results suggest that CBP, Ku70, BAX, and NOX2 form a transcriptional network to prevent cell death in human melanoma.
CELL DEATH DISCOVERY
(2021)
Article
Oncology
Pengpeng Zhang, Bohan Li, Qianfeng Chen, Hui Wang, Qing Feng
Summary: Glucose restriction induces ROS-AMPK-mediated CTR1 expression in NSCLC cells. Feeding a low-carbohydrate ketogenic diet in combination with cisplatin enhances the efficacy of cisplatin and increases survival in a mouse model.
Review
Pharmacology & Pharmacy
Sepideh Mirzaei, Aliasghar Tabatabaei Mohammadi, Mohammad Hossein Gholami, Farid Hashemi, Ali Zarrabi, Amirhossein Zabolian, Kiavash Hushmandi, Pooyan Makvandi, Marek Samec, Alena Liskova, Peter Kubatka, Noushin Nabavi, Amir Reza Aref, Milad Ashrafizadeh, Haroon Khan, Masoud Najafi
Summary: Nrf2 signaling plays a crucial role in protecting against oxidative damage, but its activation in cancer cells can lead to chemoresistance. Modulating Nrf2 signaling during cisplatin treatment can suppress the development of chemoresistance and alleviate drug toxicity. Activating Nrf2 signaling can help prevent the side effects of cisplatin.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Lude Wang, Xiaoya Zhao, Jianfei Fu, Wenxia Xu, Jianlie Yuan
Summary: Cisplatin is a commonly used chemotherapy drug in cancer treatment. However, the drug resistance of tumor cells often leads to treatment failure. Metabolic reprogramming of tumor cells plays a crucial role in tumorigenesis and development.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuai Li, Tangming Peng, Xia Zhao, Marta Silva, Linlin Liu, Wenshu Zhou, Ligang Chen, Wenhua Zheng
Summary: Ischemic stroke is a major cause of death and disability among adults, with limited treatment options. Artemether, a drug used for malaria, has been shown to improve neurological deficits and reduce infarction volume in animal models of stroke. It also promotes cell proliferation, reduces cell apoptosis, and activates signaling pathways related to neuroprotection.
Article
Biochemistry & Molecular Biology
Zihan Cui, Dapeng Li, Jun Zhao, Kai Chen
Summary: This study demonstrates that the combination of Falnidamol (FLD) and cisplatin (DDP) effectively inhibits the progression of non-small-cell lung cancer (NSCLC). FLD enhances the cytotoxicity of DDP and reduces the proliferation of NSCLC cells. The combination treatment induces cell cycle arrest, DNA damage, and apoptosis through the generation of reactive oxygen species (ROS). Furthermore, it triggers ferroptosis and inhibits epithelial to mesenchymal transition (EMT) and epidermal growth factor receptor (EGFR) phosphorylation in NSCLC cells. Animal studies confirm the therapeutic efficacy of FLD/DDP in reducing tumor growth and lung metastasis.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Clinical Neurology
Helena Motaln, Ursa Cercek, Alfred Yamoah, Priyanka Tripathi, Eleonora Aronica, Anand Goswami, Boris Rogelj
Summary: Nuclear to cytoplasmic mislocalization and aggregation of RNA-binding proteins are key features of ALS and FTLD. A novel antibody against phosphorylated FUS reveals its presence in small diffuse inclusions in the brains of FTLD patients, suggesting a potential role in FTLD pathogenesis.
Article
Oncology
So Hee Dho, Kwang-Pyo Lee, Dongjun Jeong, Chang-Jin Kim, Kyung-Sook Chung, Ji Young Kim, Bum-Chan Park, Sung Sup Park, Seon-Young Kim, Ki-Sun Kwon
Article
Oncology
So Hee Dho, Ji Young Kim, Kwang-Pyo Lee, Eun-Soo Kwon, Jae Cheong Lim, Chang-Jin Kim, Dongjun Jeong, Ki-Sun Kwon
EXPERIMENTAL CELL RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
So Hee Dho, Scott R. Manson, Sung Hee Jung, Jae Cheong Lim, Steven J. Weintraub
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Multidisciplinary Sciences
So Hee Dho, Soo Yong Kim, Chaeuk Chung, Eun Ha Cho, So-Young Lee, Ji Young Kim, Lark Kyun Kim, Sung-Won Min, Jichul Lee, Sung Hee Jung, Jae Cheong Lim
SCIENTIFIC REPORTS
(2018)
Review
Immunology
So Hee Dho, Jae Cheong Lim, Lark Kyun Kim
Article
Cell Biology
Ji Young Kim, Young-Kyu Park, Kwang-Pyo Lee, Seung-Min Lee, Tae-Wook Kang, Hee-Jin Kim, So Hee Dho, Seon-Young Kim, Ki-Sun Kwon
Article
Geriatrics & Gerontology
Seung-Min Lee, So Hee Dho, Sung-Kyu Ju, Jin-Soo Maeng, Jeong-Yoon Kim, Ki-Sun Kwon
Article
Neurosciences
Jong Seong Ha, So Hee Dho, Tae Hyun Youm, Ki-Sun Kwon, Sung Sup Park
Article
Biochemistry & Molecular Biology
So Hee Dho, Benjamin E. Deverman, Carlo Lapid, Scott R. Manson, Lu Gan, Jacob J. Riehm, Rajeev Aurora, Ki-Sun Kwon, Steven J. Weintraub
Article
Oncology
So Hee Dho, Eun Ha Cho, Ji Yeon Lee, So-Young Lee, Sung Hee Jung, Lark Kyun Kim, Jae Cheong Lim
Article
Biochemistry & Molecular Biology
SH Dho, KS Kwon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2003)
Article
Hematology
TH Lee, SU Kim, SL Yu, SH Kim, DS Park, HB Moon, SH Dho, KS Kwon, HJ Kwon, YH Han, S Jeong, SW Kang, HS Shin, KK Lee, SG Rhee, DY Yu
Article
Genetics & Heredity
L Cai, B Struk, MD Adams, W Ji, T Haaf, HL Kang, SH Dho, XQ Xu, F Ringpfeil, J Nancarrow, S Zach, L Schaen, M Stumm, TH Niu, J Chung, K Lunze, B Verrecchia, LA Goldsmith, D Viljoen, LE Figuera, W Fuchs, M Lebwohl, J Uitto, R Richards, D Hohl, R Ramesar, DF Callen, UJ Kim, NA Doggett, KH Neldner, K Lindpainter
JOURNAL OF MOLECULAR MEDICINE-JMM
(2000)