Article
Oncology
Marjolijn M. Ladan, Titia G. Meijer, Nicole S. Verkaik, Zofia M. Komar, Carolien H. M. van Deurzen, Michael A. den Bakker, Roland Kanaar, Dik C. van Gent, Agnes Jager
Summary: This study explores the feasibility of a functional sensitivity test for the chemotherapeutic agents cisplatin and docetaxel on breast cancer tissue slices in culture. The results show that these two agents need to be analyzed differently and the test has been adapted for biopsies from metastatic breast tumors. The test is now ready for evaluation of its predictive value in clinical trials.
Article
Biochemistry & Molecular Biology
Tara Zuhair Kassem, Marius Wunderle, Lukas Kuhlmann, Matthias Ruebner, Hanna Huebner, Juliane Hoyer, Andre Reis, Peter A. Fasching, Matthias W. Beckmann, Carolin C. Hack, Rainer Fietkau, Luitpold Distel
Summary: The study examines the chromosomal radiation sensitivity of BRCA1/2 mutation carriers and finds that these mutations result in slightly increased chromosomal sensitivity to radiation. The degree of chromosomal radiation sensitivity varies depending on the locus of the BRCA1/2 mutation.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexis Matute, Jessica Tabart, Jean-Paul Cheramy-Bien, Claire Kevers, Jacques Dommes, Jean-Olivier Defraigne, Joel Pincemail
Summary: This study compared the antioxidant capacities of fruit and vegetable juices using the ORAC assay and the PMA-whole blood assay, finding that the latter was more effective. The ranking of juices in terms of antioxidant capacity varied greatly between the two tests, with a stronger correlation observed between total phenolic compounds and % superoxide anion inhibition. Various phenolic compounds found in the juices were able to inhibit superoxide anion production at low concentrations, with a strong positive correlation.
Article
Engineering, Biomedical
Francesco Meani, Giuseppe Barbalace, Davide Meroni, Olivia Pagani, Ulrike Perriard, Alberto Pagnamenta, Andrea Aliverti, Emanuele Meroni
Summary: This paper presents a cancer detection procedure using customised electrical impedance spectroscopy (EIS) in breast cancer surgical samples and analyzes its outcomes. A tissue analyser was developed to measure tissue impedance in breast specimens using low-amplitude alternating current along a broad spectrum of frequencies. Experimental measurements on over one hundred excised breast cancer specimens showed significant differences in impedance between neoplastic and non-neoplastic tissues, with good reproducibility. EIS has the potential to discriminate between healthy tissue and cancer, and its further development may allow for real-time evaluation of the transition zone between cancer and normal tissues during surgery.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Muktashree Saha, Anil P. Bidkar, Siddhartha S. Ghosh
Summary: This study aimed to develop biomimetic nanocarriers for specific homologous targeting of anticancer drugs PDTC and doxorubicin, showing efficient internalization and enhanced antiproliferative effects. The drug-loaded nanovesicles demonstrated higher killing effect for homologous cell targeting compared to other cell types, indicating increased antiproliferative potency and induction of apoptosis through ROS generation. The developed membrane-derived nanocarrier is a promising biocompatible system for ex vivo targeted drug delivery.
Article
Biochemistry & Molecular Biology
Mario Cioce, Andrea Sacconi, Sara Donzelli, Claudia Bonomo, Letizia Perracchio, Mariantonia Carosi, Stefano Telera, Vito Michele Fazio, Claudio Botti, Sabrina Strano, Giovanni Blandino
Summary: Genomics has provided insights into breast cancer, but the study of metastatic breast cancer lags behind. Integrated multi-OMICS characterization and the use of clinically relevant patient-derived tumor-organoids may enhance our understanding and treatment of metastatic breast cancer.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Gastroenterology & Hepatology
Xu Zhang, Krystal Walker, Janice Mayne, Leyuan Li, Zhibin Ning, Alain Stintzi, Daniel Figeys
Summary: This study developed a rapid method for evaluating the viability of live microbiota in biobanks and found that long-term freezing in deoxygenated glycerol buffer has minimal detrimental effects on the cultivability of fecal microbiota.
Article
Oncology
Nuria Carmona-Ule, Miriam Gonzalez-Conde, Carmen Abuin, Juan F. Cueva, Patricia Palacios, Rafael Lopez-Lopez, Clotilde Costa, Ana Belen Davila-Ibanez
Summary: In this study, circulating tumor cells (CTCs) were cultured in vitro using growth factors and Nanoemulsions. The cultured CTCs showed mesenchymal and stem cell characteristics, and their culture duration was predictive of progression-free survival in breast cancer patients. The subset of CTCs capable of ex vivo growth may represent the population with metastatic potential in vivo.
Review
Biochemistry & Molecular Biology
Ana Isabel Fraguas-Sanchez, Cristina Martin-Sabroso, Ana Isabel Torres-Suarez
Summary: The chick chorioallantoic membrane (CAM) model has become a valuable tool in pharmaceutical and biological research, allowing for the evaluation of drug toxicity, testing the efficacy of anticancer and anti-infective therapies, and conducting biodistribution and tissue engineering studies.
CURRENT MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Joanna Gustalik-Nowicka, David Aebisher, Marta Adamczyk, Grzegorz Cieslar, Aleksandra Kawczyk-Krupka
Summary: The incidence and mortality of breast cancer are on the rise in society. Early detection through ultrasound, mammography, and MRI are recommended. Photodynamic therapy (PDT) is a novel approach used for both cancer diagnosis and treatment, utilizing light, oxygen, and photosensitizers to generate cytotoxic reactive oxygen species for cancer treatment, though its application in breast cancer remains limited. Preclinical studies on PDT can provide useful insights for its biomedical applications in vivo.
ACTA POLONIAE PHARMACEUTICA
(2021)
Article
Multidisciplinary Sciences
David R. Nelson, Jacqueline Brown, Aki Morikawa, Michael Method
Summary: This study aimed to investigate the impact of breast cancer subtypes on breast cancer-specific mortality in early breast cancer (EBC) and the incidence of high-risk indicators for recurrence. It also examined the mortality risk difference between patients who met/did not meet the high-risk criteria defined in the monarchE trial.
Article
Biotechnology & Applied Microbiology
Zihuan Luo, Tao Liao, Yannan Zhang, Haofeng Zheng, Qipeng Sun, Fei Han, Maolin Ma, Yongrong Ye, Qiquan Sun
Summary: This study developed a membrane-anchored protein PD-L1 (map-PD-L1) that can precisely target the allograft in organ transplantation. The results showed that map-PD-L1 can protect grafts against acute injury and reduce T cell graft infiltration, potentially providing a long-term effect in allograft protection.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Adrienne G. Waks, Esther R. Ogayo, Laia Pare, Mercedes Marin-Aguilera, Fara Braso-Maristany, Patricia Galvan, Oleguer Castillo, Olga Martinez-Saez, Ana Vivancos, Patricia Villagrasa, Guillermo Villacampa, Paolo Tarantino, Neelam Desai, Jennifer Guerriero, Otto Metzger, Nadine M. Tung, Ian E. Krop, Joel S. Parker, Charles M. Perou, Aleix Prat, Eric P. Winer, Sara M. Tolaney, Elizabeth A. Mittendorf
Summary: This study suggests that the HER2DX pCR score assay can predict the likelihood of pathologic complete response (pCR) in patients with early-stage ERBB2+ breast cancer who receive deescalated neoadjuvant therapy. The HER2DX pCR score could guide treatment decisions by identifying patients who are candidates for either deescalated or escalated approaches.
Article
Fisheries
Aaron P. Frenette, Tania Rodriguez-Ramos, Fabio Zanuzzo, Devyn Ramsay, Shawna L. Semple, Cheryl Soulliere, Tania Rodriguez-Cornejo, George Heath, Emily McKenzie, Jack Iwanczyk, Mark Bruder, Marc G. Aucoin, A. Kurt Gamperl, Brian Dixon
Summary: In this study, a quantitative enzyme-linked immunosorbent assay (ELISA) was developed to accurately measure the concentration of IL-1 beta protein in salmonids. By comparing changes in IL-1 beta protein levels to the expression of its mRNA, a more accurate and complete understanding of IL-1 beta's role in the immune response of salmonids can be achieved.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Review
Oncology
James Clark, Christina Fotopoulou, Paula Cunnea, Jonathan Krell
Summary: Epithelial ovarian cancer (EOC) is a heterogeneous disease with limited treatment options. The use of patient-derived organoids (PDO) and explant tumor slices as novel research models offers the potential for personalized treatment and improved outcomes for ovarian cancer patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Steven Lohard, Nathalie Bourgeois, Laurent Maillet, Fabien Gautier, Aurelie Fetiveau, Hamza Lasla, Frederique Nguyen, Cline Vuillier, Alison Dumont, Agnes Moreau-Aubry, Morgane Frapin, Laurent David, Delphine Loussouarn, Olivier Kerdraon, Mario Campone, Pascal Jezequel, Philippe P. Juin, Sophie Barille-Nion
NATURE COMMUNICATIONS
(2020)
Letter
Oncology
Pascal Jezequel, Anne Patsouris, Catherine Guette, Philippe P. Juin, Mario Campone
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2020)
Review
Biochemistry & Molecular Biology
Sophie Barille-Nion, Steven Lohard, Philippe P. Juin
Review
Oncology
Lisa Nocquet, Philippe P. Juin, Frederique Souaze
Article
Biochemical Research Methods
Jean-Michel Nguyen, Pascal Jezequel, Pierre Gillois, Luisa Silva, Faouda Ben Azzouz, Sophie Lambert-Lacroix, Philippe Juin, Mario Campone, Aurelie Gaultier, Alexandre Moreau-Gaudry, Daniel Antonioli
Summary: A new type of random forest was proposed, deviating from Breiman's principles, by building trees with no classification errors in large quantities, using decision trees with neurons at each node and a unique structure. A family of statistical information criteria called Nguyen information criteria (NICs) was developed to evaluate the predictive qualities of features, showing advantages over traditional methods like Akaike information criterion and support vector machines-recursive feature elimination (SVM-RFE). The inclusion of artificial neurons in tree nodes allowed for the consideration of a large number of classifiers simultaneously, resulting in perfect trees without classification errors.
Article
Biology
Fadoua Ben Azzouz, Bertrand Michel, Hamza Lasla, Wilfried Gouraud, Anne-Flore Francois, Fabien Girka, Theo Lecointre, Catherine Guerin-Charbonnel, Philippe P. Juin, Mario Campone, Pascal Jezequel
Summary: Recent studies have shown that TNBC can be divided into three subtypes, but current prediction methods are limited by batch effects and dataset dependency. This study established an absolute predictor for intra-patient diagnosis using machine learning algorithms, selecting the best model through cross-validation with a specific subset of indicators, with results indicating that the GB model based on these indicators performed the best.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Review
Oncology
Pascal Jezequel, Wilfried Gouraud, Fadoua Ben Azzouz, Agnes Basseville, Philippe P. Juin, Hamza Lasla, Mario Campone
Summary: The latest version of bc-GenExMiner is a user-friendly tool for statistical mining on breast tumor transcriptomes, with more than 20 clinicopathologic and molecular characteristics annotated. It serves its purpose in the international scientific community working in breast cancer research, with an increasing number of visits, analyses, and scientific citations.
BULLETIN DU CANCER
(2021)
Review
Biochemistry & Molecular Biology
Illa Tea, Arnaud De Luca, Anne-Marie Schiphorst, Mathilde Grand, Sophie Barille-Nion, Eric Mirallie, Delphine Drui, Michel Krempf, Regis Hankard, Guillaume Tcherkez
Summary: The natural abundance of heavy stable isotopes is important in human physiology research, as it can easily identify changes in metabolic fluxes. This suggests the potential for medical diagnosis and prognostic using isotopes at natural abundance.
Article
Mathematical & Computational Biology
Pascal Jezequel, Wilfried Gouraud, Fadoua Ben Azzouz, Catherine Guerin-Charbonnel, Philippe P. Juin, Hamza Lasla, Mario Campone
Summary: bc-GenExMiner is a breast cancer-associated web portal with a new statistical mining module that allows for differential gene expression analyses. It includes data from 62 breast cancer cohorts and one healthy breast cohort for comparison of gene expression in different tissues. The module offers various analysis categories and can be used for exploratory and validation purposes.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Meeting Abstract
Oncology
Jean-Michel Nguyen, Philippe Juin, Daniel Antonioli, Alexandre Moreau-Gaudry, Mario Campone, Pascal Jezequel
JOURNAL OF CLINICAL ONCOLOGY
(2020)
Meeting Abstract
Oncology
Anne Patsouris, Hamza Lasla, Pascal Jezequel, Philippe Juin, Alice Boissard, Cecile Henry, Mario Campone, Catherine Guette
Article
Oncology
Steven Lohard, Philippe P. Juin, Sophie Barille-Nion
MOLECULAR & CELLULAR ONCOLOGY
(2020)