Journal
ONCOTARGET
Volume 7, Issue 2, Pages 2093-2104Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.6573
Keywords
AEG-1; epithelial-mesenchymal transition; tongue squamous cell carcinoma; Wnt/PCP; metastasis
Categories
Funding
- National Natural Science Foundation of China [30900650/H1615, 81372501/H1615, 81572260/H1615, 81172232/H1615, 81172564/H1625]
- Guangdong Natural Science Foundation [2011B031800025, S2012010008378, S2012010008270, S2013010015327, 2013B021800126, 2013B021800259]
Ask authors/readers for more resources
Despite advances in therapy, survival among patients with locally advanced squamous cell carcinoma of tongue (TSCC) and cervical lymph node metastasis remains dismal. Here, we estimated the functional effect of AEG-1 on TSCC metastasis and explored the molecular mechanism by which AEG-1 stimulates epithelial-mesenchymal transition (EMT). We initially found that AEG-1 mRNA levels were much higher in metastatic TSCC than in non-metastatic TSCC and that AEG-1 expression strongly correlates with EMT status. Receiver operating characteristic analysis showed that the combined AEG-1 and EMT statuses are predictive of the survival rate among TSCC patients. In addition, AEG-1 knockdown inhibited EMT in cultured TSCC cell lines and in a xenograft-mouse model. Recombinant AEG-1 activated Wnt/PCP-Rho signaling, and its stimulatory effects on TSCC cell invasiveness and EMT were reversed by an anti-Wnt5a neutralizing antibody or by inhibition of Rac1 or ROCK. These results highlight the critical stimulatory effect of AEG-1 on cancer cell invasiveness and EMT and indicate that AEG-1 may be a useful prognostic biomarker for TSCC patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available