Article
Plant Sciences
Edinilze S. C. Oliveira, Leonard D. R. Acho, Ruben Dario Morales-Gamba, Alessandro S. do Rosario, Jose Fernando M. Barcellos, Emerson S. Lima, Marcos B. Machado
Summary: The dry extract of Myrcia multiflora leaves has a hypoglycemic effect on diabetic mice and exerts nephro- and hepatoprotective effects. It has the potential to be an adjuvant for diabetes mellitus type 2, similar to acarbose.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Plant Sciences
Glenda Laissa Oliveira de Melo Candeia, Wendao Kennedy Costa, Alisson Marcario de Oliveira, Thiago Henrique Napoleao, Patricia Maria Guedes Paiva, Magda Rhayanny Assuncao Ferreira, Luiz Alberto Lira Soares
Summary: Our study investigated the anti-inflammatory and analgesic activities of spray-dried extract and fractions from Eugenia uniflora leaves using in vivo pharmacological models in mice. The results suggest that the extract and ethyl acetate fraction exhibit peripheral and central antinociceptive activity, which may be related to the presence of flavonoids, mainly myricitrin, and the involvement of opioid receptors.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Ewelyn Cintya Felipe dos Santos, Juliana Morais Andrade de Lima, Janaina Carla Barbosa Machado, Magda Rhayanny Assuncao Ferreira, Luiz Alberto Lira Soares
Summary: The aim of this study was to assess the stability-indicating capacity of an analytical method for Eugenia uniflora and the effect of degradation on antioxidant and antifungal activities. The method was found to accurately measure myricitrin levels in SDE from E. uniflora and had stability-indicating capability. The forced degradation study showed that myricitrin is sensitive to hydrolysis but generally stable under other stress conditions. Degradation negatively affected the antioxidant activity of SDE but had no significant effect on antifungal activity. These findings can assist in quality control and optimal storage of products derived from E. uniflora.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Medicinal
V. A. Kurkin, N. Zimenkina
Summary: An HPLC method was developed for quantitative determination of myricitrin in black walnut bark, with the content of the dominant flavonoid myricitrin ranging from 3.10% to 3.18% in J. nigra bark. The error of a single determination of myricitrin in the bark was +/- 5.81% with a confidence probability of 95%.
PHARMACEUTICAL CHEMISTRY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Daniil N. Olennikov
Summary: Rhaponticum uniflorum is a plant species commonly used in Asian traditional medicines for its anti-inflammatory and stimulant properties. It contains various compounds and exhibits a wide range of biological activities. Further research, including clinical studies, is needed to better understand its medicinal value.
Article
Biochemistry & Molecular Biology
Isabela Santos Lopes, Fernando Cassas, Thiago Andre Moura Veiga, Flavia Rodrigues de Oliveira Silva, Lilia Coronato Courrol
Summary: This article presents a low-cost, highly selective, and sensitive sensor for detecting L-Cysteine (Cys) based on silver nanoparticles. The EuAgNPs synthesized by a green synthesis method showed selective detection of Cys and exhibited fast response.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Plant Sciences
Mohd Amir, Ameeduzzafar Zafar, Rizwan Ahmad, Wasim Ahmad, Mohammad Sarafroz, Mohammad Khalid, Mohammed M. Ghoneim, Sultan Alshehri, Shadma Wahab, Sayeed Ahmad, Mohd Mujeeb
Summary: The increase in the use of herbal medicines has led to more stringent regulations for quality variation and standardization. This study aimed to standardize Prunus domestica Linn. using various evaluation techniques, advanced instrumental analysis, and biological and toxicological techniques. The results showed that the fruit met quality requirements and did not contain harmful substances beyond the permissible limits.
Article
Biochemistry & Molecular Biology
Giuseppe Mannino, Graziella Serio, Alberto Asteggiano, Noemi Gatti, Cinzia M. Bertea, Claudio Medana, Carla Gentile
Summary: This study evaluated the phytochemical profile and antioxidative properties of Eugenia involucrata fruits. The results showed that these berries are a rich source of polyphenols with high radical-scavenging and metal-reducing activities. Cell-based experiments demonstrated that the fruit extract at low concentration can prevent oxidative damage and improve enzymatic antioxidant defenses. These findings suggest that Eugenia involucrata fruits could be a potential source of natural antioxidants with nutraceutical applications.
Article
Pharmacology & Pharmacy
Mousa Sha'at, Adrian Florin Spac, Iulian Stoleriu, Alexandra Bujor, Monica Stamate Cretan, Mihaela Hartan, Lacramioara Ochiuz
Summary: This study developed a simple, rapid, precise, accurate, and cost-effective HPLC method for estimating metformin hydrochloride (M-HCl) using the quality by design (QbD) approach. The experimental conditions of the HPLC method were optimized using design of experiments (DoE), and the critical method parameters were identified through risk assessment. The optimized method was validated according to ICH guidelines and successfully applied to evaluate M-HCl content in tablets and in vitro dissolution studies.
Article
Multidisciplinary Sciences
Ajay Kumar Meena, P. Rekha, Ayyam Perumal, M. Gokul, K. N. Swathi, R. Ilavarasan
Summary: The study aimed to develop standardization parameters for Ashwagandhadi lehyam, an Ayurvedic formulation, including evaluation of organoleptic characters, physico-chemical evaluation, fingerprint profiling, assessment of dominant bioactive markers, and quantification of bioactive marker Withaferin-A by HPLC. The presence of bioactive markers is identifiable through HPTLC fingerprint profiles and quantification of biomarker by HPLC, providing the best methods to assess the quality of the finished formulation.
Article
Chemistry, Applied
Sandra K. S. Macedo, Tamires S. Almeida, Jose Marcos T. Alencar Filho, Katia S. Bezerra Lima, Rafael C. Liborio, Mateus M. Costa, Pedro J. Rolim Neto, Larissa A. Rolim, Xirley P. Nunes
Summary: Triplaris gardneriana is traditionally used in medicine. In this study, the chemical composition of the leaves was analyzed, resulting in the isolation of quercetin and lupeol. The ethyl acetate fraction showed higher content of quercetin and exhibited good antibacterial activity, making it a potential alternative for producing this secondary metabolite.
NATURAL PRODUCT RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Ayodeji Oluwabunmi Oriola, Gugulethu Mathews Miya, Moganavelli Singh, Adebola Omowunmi Oyedeji
Summary: The leaves of Eugenia uniflora have been used in traditional medicine for the treatment of tumors and chronic inflammatory diseases. Two flavonol glycosides were isolated from the leaves, among which myricitrin showed potential cytotoxic and antioxidant activities, suggesting its potential as a cytotoxic agent.
SCIENTIA PHARMACEUTICA
(2023)
Article
Pharmacology & Pharmacy
Dina B. E. Farag, Carol Yousry, Abdulaziz Mohsen Al-Mahallawi, Hesham El-Askary, Meselhy R. Meselhy, Nermeen AbuBakr
Summary: This study explores the potential of incorporating Origanum majorana extract (OE) into nano-sized systems to enhance its biological effects and treat salivary gland dysfunction caused by diabetes. The results demonstrate that the nano-cubosomal systems significantly reduce blood glucose levels, improve gland histopathology, and show promising gene expression analysis. Therefore, cubosomal nano-systems could be considered as potential carriers for effective OE treatment.
Article
Integrative & Complementary Medicine
Viviana S. Bravi, Sandra Castello, Luis Bruno-Blanch
Summary: The compounds in the extract from Eugenia uniflora L. leaves were separated and analyzed using chromatographic systems. The structures of bornyl acetate and germacrone sesquiterpene were identified, and ursolic acid and pulegone were also found.
BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
(2023)
Article
Chemistry, Applied
Pradip Debnath, Bhaskar Das, Seha Singha, Amit Kar, Pallab Kanti Haldar, Nanaocha Sharma, Pulok K. Mukherjee
Summary: This study aimed to quantify the cucurbitacin E content in five different varieties of melon fruit using a validated RP-HPLC method. The study found variations in the cucurbitacin E content among the five different varieties of melon fruits. This method shows potential in addressing quality-related aspects of medicinal food plants from the Cucurbitaceae family and its derived products.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Jose Williams Gomes de Oliveira Filho, Teresinha de Jesus Aguiar dos Santos Andrade, Rosalia Maria Torres de Lima, Dulce Helena Siqueira Silva, Antonielly Campinho dos Reis, Jose Victor de Oliveira Santos, Ag-Anne Pereira Melo de Meneses, Ricardo Melo de Carvalho, Ana Maria Oliveira da Mata, Marcus Vinicius Oliveira Barros de Alencar, Ana Carolina Soares Dias, Felipe Cavalcanti Carneiro da Silva, Muhammad Torequl Islam, Cain C. T. Clark, Joao Marcelo de Castro e Sousa, Ana Amelia de Carvalho Melo-Cavalcante
Summary: This study evaluated the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract, citrinin and dicitrinin-A of Penicillium citrinum. The results showed that these substances exhibited toxicity, cytotoxicity and mutagenicity in the test systems, possibly through oxidative stress induction pathway.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Chemistry, Applied
Nerilson M. Lima, Teresinha J. A. S. Andrade, Dulce Helena S. Silva
Summary: The study identified sixteen compounds from Inga edulis leaves extract using dereplication strategy, including triterpenes, flavonoids, phenolic acids, and anthocyanins. The species showed strong antioxidant potential with phenolic compounds as major constituents. The techniques used in this study provided effective strategies for the early detection of active natural products from plant extracts.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Applied
Nerilson M. Lima, Sara R. de Marqui, Teresinha de Jesus A. S. Andrade, Dulce H. S. Silva
Summary: Natural products provide a rich source for therapeutic discovery, and this study aimed to obtain metabolic profiling from ethanol extract ofInga semialata leaves and identify metabolites in mixture using co-injection experiments and NMR spectroscopy.
NATURAL PRODUCT RESEARCH
(2022)
Review
Plant Sciences
Cristiano S. Funari, Daniel Rinaldo, Vanderlan S. Bolzani, Robert Verpoorte
Summary: This review article investigates the response of phytochemists to green chemistry insights since the launch of the Pollution Prevention Act in 1990. Highly cited phytochemistry papers from each year between 1990 and 2019 were sampled to assess the greenness of experimental procedures, particularly regarding the use of solvents. Progress in sustainability was observed in the extraction stage, but not in purification procedures, where hazardous solvents like chloroform and dichloromethane were still commonly used. Potential alternative approaches for more sustainable purification procedures are discussed, along with initiatives for a broader sustainable phytochemical research.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Agronomy
Danubia Aparecida Costa Nobre, Rodrigo Rocha Silva, Vinicius Guimaraes Nasser, Geraldo Humberto Silva, Leonardo Angelo Aquino, Willian Macedo
Summary: This study examines the interactions between plant physiology and seed chemical composition of soybean cultivars under different spatial arrangements. Two soybean cultivars were grown in four different arrangements, and the gas exchange, seed quality, and chemical composition were analyzed. The results showed that the layout with 40.0 cm between rows and 8.9 cm between plants was optimal for Tec 7849 Ipro, while the layout with 50.0 cm between rows and 5.3 cm between plants was ideal for Monsoy 6972 Ipro.
REVISTA DE AGRICULTURA NEOTROPICAL
(2023)
Article
Plant Sciences
Helena Mannochio-Russo, Wilhan D. G. Nunes, Rafael F. Almeida, Lorena C. Albernaz, Laila S. Espindola, Vanderlan S. Bolzani
Summary: This study investigated secondary metabolites with larvicidal activity from the Malpighiaceae taxon using metabolomic and bioactivity-based methods. The results revealed significant differences in the metabolic profiles of different plant organs and collection sites. By employing a bioguided approach, three larvicidal compounds were isolated. These findings support the use of metabolomic-guided approaches in combination with classical phytochemical techniques for the search of natural larvicidal compounds for vector control.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Agronomy
Gabriel Tadeu de Paiva Silva, Karolina Gomes Figueiredo, Dejane Santos Alves, Denilson Ferreira de Oliveira, Geraldo Humberto Silva, Gabriela Trindade Silva, Murilo Silva de Oliveira, Antonio Biondi, Geraldo Andrade Carvalho
Summary: The toxicity of essential oils from lime, petitgrain, and bergamot, as well as their major compounds, against T. absoluta was evaluated. The results showed that the essential oils were toxic to T. absoluta, and low concentrations caused deleterious effects on its reproductive and population parameters.
Article
Agronomy
Mayara Ketllyn de Paula Rosetti, Dejane Santos Alves, Isabela Caroline Luft, Katiane Pompermayer, Andressa Soares Scolari, Gabriela Trindade Silva, Murilo Silva de Oliveira, Javier Andres Garcia Vanegas, Horacio Bambo Pacule, Geraldo Humberto Silva, Denilson Ferreira de Oliveira, Geraldo Andrade Carvalho
Summary: This study evaluated the toxicity of essential oils from plants of the Annona neolaurifolia, Duguetia lanceolata, and Xylopia brasiliensis species on the fall armyworm (FAW) and its natural enemy, Trichogramma pretiosum. The essential oils from the leaves and stem bark of Duguetia lanceolata were the most effective against FAW, and they contained beta-caryophyllene and caryophyllene oxide as major compounds that reduced the insect's survival probability. Additionally, the essential oil from Duguetia lanceolata leaves showed low toxicity to the egg-larva stage of Trichogramma pretiosum, suggesting its potential for controlling FAW.
Article
Parasitology
Aline Coqueiro, Daniara Cristina Fernandes, Amanda Danuello, Luis Octavio Regasini, Elaine Monteiro Cardoso-Lopes, Maria Claudia Marx Young, Luce Maria Brandao Torres, Vicente Paulo Campos, Dulce Helena Siqueira Silva, Vanderlan da Silva Bolzani, Denilson Ferreira de Oliveira
Summary: Although new nematicides have emerged, there is still high demand for less toxic and more efficient products to control plant-parasitic nematodes. As a result, increased research on natural secondary metabolites from plants to develop new nematicides has been observed. In this study, extracts from eleven Brazilian plant species were screened, and the extract from Piterogyne nitens showed potent nematostatic activity. The alkaloid fraction obtained from the ethanol extract of P. nitens was more active, and three isoprenylated guanidine alkaloids isolated from this fraction showed similar activity, indicating their potential for the development of new products to control Meloidogyne incognita.
EXPERIMENTAL PARASITOLOGY
(2023)
Article
Computer Science, Artificial Intelligence
Rafael Vieira, Kally Alves de Sousa, Givaldo Souza da Silva, Dulce Helena Siqueira Silva, Ian Castro-Gamboa
Summary: CHEIC is a web platform that uses convolutional neural networks to identify volatile molecules from microorganisms with potential against respiratory and bronchopulmonary diseases. The platform allows users to find volatile molecules with biological activity through molecular docking. It presents a Deep Learning model with 93% accuracy in classifying volatile compounds and candidate molecules for respiratory disease drugs. The model identified 39 drug-like molecules out of 548 tested. Combining molecular docking results with molecular dynamics analysis, four volatile compounds were found with the potential to modulate proteins associated with respiratory tract diseases. CHEIC is a free and promising tool for finding therapeutic agents for respiratory diseases and providing insights for researchers interested in omics sciences.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Haroon Ur Rashid, Vanderlan S. Bolzani, Khalid Khan, Luiz Antonio Dutra, Nasir Ahmad, Abdul Wadood
Summary: In this study, a stable three-dimensional structure of alpha-glucosidase was designed through in silico analysis, which may aid in the development of effective antifungal drugs.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Natalia Felix Alves, Dayane Castro Ribeiro, Helena Mannochio-Russo, Suzana Aparecida Silva Queiroz, Guilherme Juliao Zocolo, Vanderlan da Silva Bolzani, Marilia Valli
Summary: Advanced Glycation End Products (AGEs) are compounds formed during hyperglycemic conditions and aging, and are associated with skin aging, loss of elasticity, and chronic diseases such as diabetes and Alzheimer's. Herbal supplements have been shown to have beneficial effects on glycemic control and oxidative stress, reducing the formation of AGEs. This study investigated natural compounds from Talisia esculenta with antiglycation activity, and identified rutin and quercitrin as compounds with significant antiglycation activity. These findings suggest the potential use of these natural compounds as AGEs inhibitors in pharmaceuticals and cosmetics.
REVISTA VIRTUAL DE QUIMICA
(2023)
Article
Chemistry, Multidisciplinary
Elcio Daniel S. Barros, Evaldo S. Moncao Filho, Mariluce G. Fonseca, Patricia S. Alves, Antonia Laires S. Santos, Chistiane M. Feitosa, Dulce Helena S. Silva, Pedro Mikael S. Costa, Claudia Pessoa, Carmem D. L. Campos, Cristina A. Monteiro, Mariana Helena Chaves, Gerardo M. Vieira Junior
Summary: This study aimed to investigate the metabolomic profile and biological potential of the secretion from the paratoid glands of Rhinella granulosa. The secretion contained biologically active compounds with high toxicity and varied chemical composition. The results showed cytotoxicity against tumor cell lines, unsatisfactory antimicrobial potential, and inhibition of the enzyme acetylcholinesterase. This study provided important information about the paratoid secretion and contributed to understanding the metabolomic and biological potential of R. granulosa.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Mario F. C. Santos, Edvaldo S. Costa, Humberto Matos, Raquel P. Morais-Urano, Geraldo H. Silva
Summary: Kojic acid, produced by different types of fungi, has great potential for industrial application. In this study, the fungus Aspergillus sp. isolated from the leaves of Hancornia speciosa was found to yield a kojic acid-rich ethyl acetate extract, which exhibited potent antioxidant activity.
REVISTA VIRTUAL DE QUIMICA
(2023)
Review
Biodiversity Conservation
Dulce Helena Siqueira Silva, Helena Mannochio-Russo, Joao Henrique Ghilardi Lago, Paula Carolina Pires Bueno, Rebeca Previate Medina, Vanderlan da Silva Bolzani, Wagner Vilegas, Wilhan Donizete Goncalves Nunes
Summary: With the support of FAPESP, Brazil has made significant progress in the research on natural products. The establishment of the Laboratory of Chemistry of Natural Products and the launch of the BIOTA-FAPESP program have contributed to the sustainable exploitation of biodiversity. Through chemical analysis, genetic assessment, and pharmacological research, a deeper understanding of the role of natural products in different biological models has been achieved. The development of omics approaches has provided new avenues for comprehensive research. The program also focuses on environmental protection and the principles of Green Chemistry, and has fostered international collaboration and industry partnerships, making important contributions to the development of the bioeconomy and support for public policies.
