Article
Food Science & Technology
Lu Tian, Xuyang Wang, Rongjie Liu, Di Zhang, Xin Wang, Runcong Sun, Wenyao Guo, Siqi Yang, Hui Li, Guoli Gong
Summary: Thymol has a broad-spectrum antibacterial effect, and this study revealed its antimicrobial mechanism against Enterobacter sakazakii by disrupting cell membranes and interfering with intracellular homeostasis. The results suggest the potential of thymol to prevent bacterial contamination by E. sakazakii in the food industry.
Article
Food Science & Technology
Haiyan Zeng, Chengsi Li, Dandan Luo, Jumei Zhang, Yu Ding, Moutong Chen, Xiaojuan Yang, Tao Lei, Shi Wu, Qinghua Ye, Rui Pang, Qihui Gu, Qingping Wu
Summary: A novel lytic phage B1 was isolated from river water in Guangzhou, China, which can simultaneously lyse C. malonaticus and C. turicensis, showing potential for controlling foodborne pathogens. The phage has high stability, strong lytic ability, and can significantly inhibit the growth of Cronobacter spp., especially C. turicensis, making it a promising candidate for food safety.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Food Science & Technology
Xue Qin, Yitian Wang, Hongxuan Li, Xinyan Yang, Zhenghui Wang, Qing Chen, Chaoxin Man, Yujun Jiang
Summary: A visual detection strategy based on DNAzyme and asymmetry recombinase polymerase amplification (aRPA) was developed for monitoring Cronobacter sakazakii in powdered infant formula (PIF). The method showed low limits of detection and good stability in on-site simulation evaluation.
Article
Food Science & Technology
Na Ling, Dexin Ou, Yizhong Shen, Danfeng Zhang, Yaping Wang, Liaowang Tong, Yu Ding, Juan Wang, Xiaojuan Yang, Jumei Zhang, Qingping Wu, Yingwang Ye
Summary: Research has shown that tolerance and biofilm formation of C. malonaticus under oxidative stress were affected when luxS gene was knocked out. The protein expression profile revealed that luxS may mediate oxidative tolerance by regulating glutathione/thioredoxin metabolism and Fe-S cluster proteins. Interestingly, the absence of luxS only reduced the swimming motility of C. malonaticus under oxidative stress.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Immunology
Katie Phair, Sonia Gonsalves Pereira, Carmel Kealey, Seamus Fanning, Damien B. Brady
Summary: Cronobacter species enhance their virulence through various mechanisms during survival in the environment, infection of hosts, and persistence inside hosts. Cronobacter sakazakii is associated with fatal infections in populations such as neonates and infants.
MICROBIAL PATHOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Zhenbo Xu, Ziqi Liu, Thanapop Soteyome, Jingjing Hua, Liang Zhang, Lei Yuan, Yanrui Ye, Zhao Cai, Liang Yang, Ling Chen, Janette M. Harro, Birthe Veno Kjellerup, Junyan Liu, Yanyan Li
Summary: The study investigated the transcriptional regulation of the Cronobacter sakazakii pmrA mutant during biofilm formation, revealing important genes involved in different stages of biofilm formation.
Article
Multidisciplinary Sciences
Ayaka Nakamura, Hajime Takahashi, Maki Arai, Tomoki Tsuchiya, Shohei Wada, Yuki Fujimoto, Yoshiomi Shimabara, Takashi Kuda, Bon Kimura
Summary: The study used cgMLST analysis to track the source of E. coli contamination in a food manufacturing plant, identifying different genogroups of E. coli in the environment and products, and estimating the transmission pathways of E. coli in the manufacturing plant. The results suggest that cgMLST analysis can be effectively used for hygiene management in food manufacturing locations.
Article
Food Science & Technology
Lingli Luo, Lanhua Yi, Jiaxin Chen, Bianfang Liu, Xin Lu
Summary: Bacteriocin BM1157 inhibits Gram-negative bacteria by disrupting their metabolic processes, inhibiting biofilm formation, and ultimately causing cell death.
Article
Food Science & Technology
Yufei Fan, Ping Li, Dongdong Zhu, Chumin Zhao, Jingbo Jiao, Xuemeng Ji, Xinjun Du
Summary: This study aims to elucidate the precise roles of the ESA_00986 gene in C. sakazakii by generating gene knockout mutants and complementary strains. The results showed that the ESA_00986 gene plays a positive role in biofilm formation, cell adhesion and invasion, and virulence, demonstrating it as a novel virulence factor and advancing our understanding of the pathogenic mechanism of C. sakazakii.
Article
Food Science & Technology
Yan Ma, Yingying Zhang, Zhongguo Shan, Xin Wang, Xiaodong Xia
Summary: The study investigated the impact of PhoP/PhoQ two component system on biofilm formation in Cronobacter sakazakii BAA-894. Results showed that the system played crucial roles in regulating gene expression, cell viability, and extracellular polymeric substance content, suggesting it as a potential target for controlling biofilms.
Article
Food Science & Technology
Sung B. Im, Sonali Gupta, Mani Jain, Aroon T. Chande, Heather A. Carleton, I. King Jordan, Lavanya Rishishwar
Summary: Foodborne pathogens are a major public health burden in the United States, and since 1996, PulseNet has been using molecular subtyping and serotyping methods to reduce the burden of foodborne illness. In 2019, PulseNet adopted whole genome sequencing to replace PFGE for higher-resolution methods.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2021)
Article
Multidisciplinary Sciences
Jale Boral, Zeliha Genc, Fatihan Pinarlik, Guz Ekinci, Mert A. Kuskucu, Pelin Irkoren, Mahir Kapmaz, Suda Tekin, Nahit Cakar, Evren Senturk, Fatma Yurdakul, Bilge Dikenelli, Fusun Can, Onder Ergonul
Summary: This study describes the increased rate of Acinetobacter baumannii infections during the COVID-19 pandemic and defines its significance within the last five years. The findings show that the rate of A. baumannii infection during the pandemic was 1.90 times higher compared to the pre-pandemic era. The introduction of enhanced and customized infection control measures has resulted in the containment of an A. baumannii outbreak.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Emily E. Jackson, Nathalie Gnanou Besse, Rabeb Bennour Hennekinne, Timothy Ells, Michel Laurentie, Thomas Meheut, Stephen J. Forsythe
Summary: This study reports the sequence types of Cronobacter sakazakii strains and investigates the correlation between sequence type and growth rate. The results suggest that there is no strong correlation between the two factors.
Review
Microbiology
Zoubida Dendani Chadi, Marie-Anne Arcangioli
Summary: DNA fingerprinting using PFGE is a widely used method to separate large DNA molecules and distinguish between different strains. It involves isolating intact chromosomal DNA and cutting it into restriction fragments for comparison. PFGE analysis provides valuable information for epidemiological investigations of bacterial pathogens.
