Journal
REVIEWS IN MEDICAL VIROLOGY
Volume 29, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/rmv.2009
Keywords
CAS9; CRISPR-CAS system; DNA Viruses; Gene Editing
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The recent development of the Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a genome editing system, has many potential applications in virology. The possibility of introducing site specific breaks has provided new possibilities to precisely manipulate viral genomics. Here, we provide diagrams to summarize the steps involved in the process. We also systematically review recent applications of the CRISPR/Cas9 system for manipulation of DNA virus genomics and discuss the therapeutic potential of the system to treat viral diseases.
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