Article
Physics, Applied
Jun-Tian Ye, Chao Yu, Wenwen Li, Zheng-Ping Li, Hai Lu, Rong Zhang, Jun Zhang, Feihu Xu, Jian-Wei Pan
Summary: We demonstrate photon-counting single-pixel imaging in the ultraviolet region using a 4H-SiC single-photon avalanche diode (SPAD) as a high-performance compact single-photon detector. By developing a tailored readout circuit with active hold-off time, we restrain detector noise and operate the SPAD in the free-running mode. With structured illumination, we reconstruct compressed images at a 4 fps frame rate, demonstrating the capability of ultraviolet imaging applications.
APPLIED PHYSICS LETTERS
(2023)
Article
Optics
Daoyu LI, Zhijie Gao, Liheng Bian
Summary: In this work, a novel sparse single-pixel imaging scheme and reconstruction algorithm are proposed to image target scenes at above 1K resolution with reduced measurements. Experimental results demonstrate the technique's superior accuracy and efficiency.
Article
Engineering, Electrical & Electronic
Cheng Zhou, Xuan Liu, Xinwei Li, Yueshu Feng, Gangcheng Wang, Jipeng Huang, Haizhu Sun, Yongli Zhang, Lijun Song
Summary: The multisensor system based on single-pixel imaging can simultaneously measure and image various optical information using a novel multispectral SPI method. It decouples the spatial and spectral dimensions, reducing the complexity of traditional spectral imaging systems. This work enables more convenient research in spectral information acquisition.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Analytical
Fan Jia, Zijing Zhang, Yuan Zhao
Summary: Ghost imaging is a technique that improves the signal-to-noise ratio and resolution by using the correlation between reference and signal arms to obtain intensity images of targets. This paper proposes a new scheme for obtaining velocity images of moving targets using the slice difference method. The experimental results show good agreement with the theoretical analysis, and a velocity image with 64 x 64 resolution is obtained. This scheme fulfils an urgent need for the detection of moving targets and has potential applications in target attitude perception and security monitoring.
Article
Computer Science, Artificial Intelligence
Jiaxu Miao, Yunchao Wei, Xiaohan Wang, Yi Yang
Summary: This study introduces a large-scale video scene parsing dataset, VSPW, which contains 251,633 frames from 3,536 videos with pixel-level annotations. It covers 231 scenes and 124 object categories. The study also proposes a Temporal Attention Blending (TAB) network to improve pixel-level semantic understanding of videos. Experimental results demonstrate the superiority of the TAB approach on the VSPW dataset.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2023)
Article
Optics
Lourdes Lopez-Garcia, William Cruz-Santos, Anmi Garcia-Arellano, Pedro Filio-Aguilar, Jose A. Cisneros-Martinez, Ruben Ramos-Garcia
Summary: Single-pixel imaging is a technique that reconstructs an image by projecting spatial patterns on an object and capturing the reflected light. Recent advancements have allowed for image reconstruction at low sampling rates using compressed sensing and Hadamard basis patterns. This study proposes an efficient strategy for ordering Hadamard patterns and reconstructing images at low sampling rates, demonstrating competitive performance.
Article
Optics
Gongxin Yao, Yiwei Chen, Yong Liu, Xiaomin Hu, Yu Pan
Summary: This paper proposes a pixel-wise residual shrinkage network for photon-efficient imaging from high-noise data. It improves the accuracy and precision of depth estimation by denoising with soft thresholding and pixel-wise classification, and demonstrates superior performance on simulated and real-world datasets.
Article
Optics
Guoqing Wang, Fang Zhao, Dongrui Xiao, Liyang Shao, Yuan Zhou, Feihong Yu, Weizhi Wang, Huanhuan Liu, Chao Wang, Rui Min, Zhijun Yan, Perry Ping Shum
Summary: The study presents a single-pixel imaging system based on the STEAM structure, which solves the issues of big data and low efficiency, while achieving data compression and increasing imaging efficiency.
Article
Optics
Ziheng Qiu, Xinyi Guo, Tian'ao Lu, Pan Qi, Zibang Zhang, Jingang Zhong
Summary: Fourier single-pixel imaging (FSI) technique allows reconstruction of images by acquiring their Fourier spectrum using a single-pixel detector. A new sampling strategy is proposed to achieve clear and sharp image reconstruction with reduced measurements, enabling efficient spatial information acquisition. The method demonstrates the ability to reconstruct a sharp and clear 256 x 256 pixel image with a sampling ratio of 10%, providing a new approach for fast single-pixel imaging.
Article
Optics
Qianjin Xiang, Yan Tang, Xiaolong Cheng, Chenhaolei Han, Yuliang Long, Lixin Zhao, Yong Yang, Jinhua Feng
Summary: Fourier single-pixel imaging (FSI) is a cost-effective imaging technique that has gained attention in recent years. This paper proposes a new sampling strategy for FSI to balance noise suppression and image details. The proposed method considers the generality and uniqueness of the spectral distribution to capture more key Fourier coefficients and retain more details with lower noise.
Article
Nuclear Science & Technology
Erik H. M. Heijne
Summary: This article discusses the evolution of early semiconductor detectors towards modern radiation imaging instruments, focusing on the role of Medipix and Timepix assemblies in this evolution. Imaging the impacts in the detecting matrix from individual ionizing particles and photons can be used to study elementary quanta and visualize characteristics of objects under irradiation.
RADIATION MEASUREMENTS
(2021)
Article
Engineering, Biomedical
Zhiyang Yao, Yongshun Xiao, Minghao Dong, Heng Deng
Summary: In this study, a lightweight, high pixel density two-layer LYSO Compton camera prototype was built, and pixel segmentation and double encoding techniques were used for detector construction and calibration. The experimental results showed that the prototype has high position resolution and detection efficiency, making it suitable for nuclear medical imaging.
PHYSICS IN MEDICINE AND BIOLOGY
(2023)
Article
Optics
Ye Tian, Ying Fu, Jun Zhang
Summary: In this paper, we propose an effective method based on channel attention convolutional neural network for under-sampled single-pixel imaging (SPI) to reconstruct high-quality object images directly from SPI measurements. The method takes advantage of unsupervised deep learning and effectively avoids over-fitting problem using SPI model constraint and total variation regularization. Extensive experimental results on simulation and real data demonstrate that the proposed method has superior performance in image quality, noise robustness, and generalization compared with the state-of-the-art SPI methods.
OPTICS AND LASER TECHNOLOGY
(2023)
Article
Optics
Guozhong Lei, Wenchang Lai, Qi Meng, Hao Liu, Dongfeng Shi, Wenda Cui, Kai Han
Summary: An efficient and noise-resistant single-pixel imaging technique based on Pseudo-Zernike moments (PZ-SPI) is proposed in this paper. By modulating the illumination light fields and measuring the reflected light intensities, the object image can be reconstructed iteratively using the Pseudo-Zernike polynomials and moments. PZ-SPI shows good performance in low sampling ratios and background noise, expanding its application in complex environments.
Article
Optics
Xi Zhang, Suhas P. Veetil, Yan Kong, Xiaoliang He, Zhilong Jiang, Shouyu Wang, Cheng Liu
Summary: A transmission K-domain transform method is proposed for multi-slice imaging using a light sheet and a high pass filter. The method computes the image of the illuminated sample volume at each scanning location using a 2D K-domain transform algorithm, allowing for sequential scanning of the sample through the light sheet. The method offers advantages for short wavelength applications where reflection is absent and reduces the need for rotation and mechanical precision.
OPTICS COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Sylvia P. Nauta, Pascal Huysmans, Gabrielle J. M. Tuijthof, Gert B. Eijkel, Martijn Poeze, Tiffany Porta Siegel, Ron M. A. Heeren
Summary: The developed 3D mass spectrometry scanner enables automated sampling and imaging of uneven topography surfaces with LA-REIMS, allowing for 3D visualization of molecular distributions on these surfaces.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Brenda Bakker, Rianne D. W. Vaes, Merel R. Aberle, Tessa Welbers, Thomas Hankemeier, Sander S. Rensen, Steven W. M. Olde Damink, Ron M. A. Heeren
Summary: This protocol describes how to prepare organoid samples for mass spectrometry imaging, which is a powerful analytical method for detecting a wide variety of molecules in a single experiment while retaining their spatiotemporal distribution.
Article
Biochemical Research Methods
Therese S. Hoiem, Maria K. Andersen, Marta Martin-Lorenzo, Remi Longuespee, Britt S. R. Claes, Anna Nordborg, Frederic Dewez, Benjamin Balluff, Marco Giampa, Animesh Sharma, Lars Hagen, Ron M. A. Heeren, Tone F. Bathen, Guro F. Giskeodegard, Sebastian Krossa, May-Britt Tessem
Summary: MALDI MS imaging is a powerful tool for spatial peptide detection in heterogeneous tissues, and proper sample preparation is crucial for high-quality measurements. In this study, an optimized protocol was developed for spatially resolved proteolytic peptide detection in fresh frozen prostate tissue sections using MALDI time-of-flight MSI.
Article
Optics
Tobias Witting, Mikhail Osolodkov, Felix Schell, Felipe Morales, Serguei Patchkovskii, Peter Susnjar, Fabio H. M. Cavalcante, Carmen S. Menoni, Claus P. Schulz, Federico J. Furch, Marc J. J. Vrakking
Summary: Researchers have generated and fully characterized extreme ultraviolet (XUV) attosecond pulses at a repetition rate of 100 kHz using near-single-cycle pulses. The high number of 10^6 XUV photons per pulse enables attosecond electron streaking experiments, revealing that the XUV pulses consist of a dominant single attosecond pulse.
Article
Optics
Marc J. J. Vrakking
Summary: This study investigates the ion + photoelectron entanglement induced by extreme ultra-violet (XUV) attosecond laser pulses in the photoionization process and its effect on the vibrational dynamics of H-2 (+). The research shows that chirping the attosecond laser pulse results in a transition from a pure state to a mixed state. The coherent vibrational dynamics can be recovered by analyzing the relationship between XUV-UV delay-dependent kinetic energy release and the kinetic energy of the accompanying photoelectron.
JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS
(2022)
Article
Chemistry, Analytical
Britt S. R. Claes, Kasper K. Krestensen, Gargey Yagnik, Andrej Grgic, Christel Kuik, Mark J. Lim, Kenneth J. Rothschild, Michiel Vandenbosch, Ron M. A. Heeren
Summary: A novel technology called MALDIIHC was published, which combined MALDI-MSI and IHC to achieve targeted imaging of biomolecules in tissue. The utility of targeted MALDIIHC and its complementarity with untargeted on-tissue proteomics was explored using breast cancer tissue. MALDI-2 was also investigated and demonstrated to improve MALDI-IHC. The combination of multiplexed MALDI-IHC with image-guided proteomics showed great potential in studying diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Felix Brausse, Marc J. J. Vrakking, Arnaud Rouzee
Summary: We investigate the photoionization of CF3I molecules in the energy range of 10 eV to 40 eV using angle-resolved photoelectron spectroscopy in the molecular frame. By using a femtosecond laser pulse, the molecules are aligned before ionization with extreme ultraviolet photon energies generated by high-harmonic generation. Non-relativistic quantum-mechanical photoionization calculations with the ePolyScat software package, considering all open ionization channels, reproduce well the manifestation of molecular alignment in photoelectron angular distributions (PADs). Our analysis reveals two dominant shape resonances that largely explain the observed changes in the PADs as a function of molecular alignment.
JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS
(2023)
Article
Biochemical Research Methods
Kasper Krijnen, Joel D. Keelor, Sebastian Boehm, Shane R. Ellis, Claus Koester, Jens Hoehndorf, Ron M. A. Heeren, Ian G. M. Anthony
Summary: Mass spectrometry imaging (MSI) is a surface analysis technique used for biological research, and multimodal imaging combining multiple imaging modes can provide a more comprehensive view of samples. In this study, a prototype Bruker timsTOF fleX instrument was modified to include secondary ion mass spectrometry (SIMS) and secondary electron (SE) imaging capabilities while preserving matrix-assisted laser desorption/ionization (MALDI) capability. The modified instrument showed improved efficiency in multimodal imaging, allowing for easy registration of images and eliminating the need for sample transfer.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Andrej Grgic, Kasper K. Krestensen, Ron M. A. Heeren
Summary: Glycans play diverse roles in biology, and variations in N-glycosylation state within tissues have clinical diagnostic value. A protocol for MALDI MSI of N-glycans from fresh frozen tissue was developed, matching the current standard of FFPE analysis. This protocol significantly improves signal intensity and spatial resolution, enhancing the clinical application of MALDI MSI for N-glycan analysis.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Britt S. R. Claes, Andrew P. Bowman, Berwyck L. J. Poad, Ron M. A. Heeren, Stephen J. Blanksby, Shane R. Ellis
Summary: The biological functions of lipids highly depend on their molecular structures, specifically even small changes in structure such as different positions of unsaturation serve as critical indicators for changes in metabolism. However, conventional mass spectrometry imaging faces challenges in differentiating lipid isomers with mixture and structural complexity. Recent advances in ozone-induced dissociation coupled with matrix-assisted laser desorption/ionization have shown potential in mapping individual double-bond isomers and visualizing lipid desaturation in adjacent tissue types. This study employs a high-speed gas-phase reaction between ionized lipids and ozone to interrogate anionic glycerophospholipids isomers in biological tissues, providing valuable information on unsaturation and acyl chain composition from a single mass spectrum.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Polymer Science
Lidia Molina-Millan, Aljoscha Koerber, Bryn Flinders, Berta Cillero-Pastor, Eva Cuypers, Ron M. A. Heeren
Summary: Synthetic polymers, when improperly disposed of, accumulate in the environment and pose potential health risks by degrading into micro- and nanoparticles (MNPs). Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) can be used to study biological or toxicological changes in organisms exposed to MNPs. MALDI-2 has been found to enhance the sensitivity of polymer analysis, making it a promising tool for studying the distribution of polymers in biological systems.
Article
Microbiology
Alison J. Scott, Alexis A. Smith, Ron M. A. Heeren, Utpal Pal, Robert K. Ernst
Summary: Spatially aware de novo discovery methods play a crucial role in identifying therapeutic targets in complex interphylum interactions. This study evaluated the potential of matrix-assisted desorption/ionization mass spectrometry imaging (MALDI-MSI) as a spatial omics method to simultaneously profile an arthropod vector and a mammalian skin in a bite model. The results demonstrated the feasibility of using MSI to analyze lipids and observe lipid reorganization at the bite site in both the tick and mammalian skin.
Article
Chemistry, Analytical
Britt S. R. Claes, Kasper K. Krestensen, Gargey Yagnik, Andrej Grgic, Christel Kuik, Mark J. Lim, Kenneth J. Rothschild, Michiel Vandenbosch, Ron M. A. Heeren
Summary: A novel technique called MALDIIHC was published recently, which combines MALDI-MSI and IHC to achieve targeted imaging of biomolecules in tissue. This study explored the utility of targeted MALDIIHC and its complementarity with untargeted spatial proteomics using breast cancer tissue. The combination of multiplexed MALDI-IHC with image-guided proteomics showed great potential for disease investigation. Additionally, the effect of MALDI-2 in improving MALDI-IHC was investigated.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Mudita Vats, Berta Cillero-Pastor, Bryn Flinders, Eva Cuypers, Ron M. A. Heeren
Summary: This study utilized MALDI-MSI to determine the spatial distribution of flavor compounds in edible button mushrooms and optimized the sample preparation protocol and investigated the effect of heat on the distribution.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Review
Chemistry, Analytical
Kasper K. Krestensen, Ron M. A. Heeren, Benjamin Balluff
Summary: Mass spectrometry imaging (MSI) has evolved from a niche technique to a widely used spatial biology tool. This review provides an overview of state-of-the-art MSI applications in both established and emerging biomedical fields, serving as a reference framework for new researchers in the field.
