Article
Chemistry, Medicinal
Takuma Kato, Hiroaki Numa, Mihoko Nakamachi, Akiko Asano, Mitsunobu Doi
Summary: Cell-penetrating peptides based on the pep-1 sequence were synthesized by replacing hydrophilic amino acids with hydrophobic amino acids, resulting in improved stability and membrane permeability.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Li-Hua Yan, Yu-Jie Zhang, Hai-Jie Hu, Chuan Zhang, Yue Wang, Xue-Tian Xu, Tong-Cun Zhang, Rui Su, Xue-Gang Luo
Summary: A novel hyaluronic acid binding peptide (HaBP) is designed and combined with Pep-1 to enhance the cell-penetrating efficiency of HA. The results show that HaBP can bind well with HA, and the combination of Pep-1 and HaBP can effectively improve the transmembrane ability of HA. This study provides a new method to promote the transmembrane and transdermal absorption of HA, which has implications for the development of hyaluronic acid and its related cosmetics or drugs.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Medicine, Research & Experimental
Zheng Li, Jihong Xing
Summary: Cardiopulmonary resuscitation (CPR) and related life support technologies have improved in recent years, but mortality and disability rates from cardiac arrest (CA) remain high. This review discusses the double-hit model of cerebral ischemia-reperfusion injury (CIRI) and the critical role of mitochondria in this process. Mitophagy, the removal of damaged mitochondria, is a key mechanism for treating CIRI after CA. In-depth studies of mitophagy regulation will contribute to the development of new therapeutic strategies.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Endocrinology & Metabolism
Fernando J. Ferrier, Isabel Saul, Nathalie Khoury, Alexander J. Ruiz, Efrain J. Perez Lao, Iris Escobar, Kunjan R. Dave, Juan Young, Miguel A. Perez-Pinzon
Summary: CA survivors often suffer from long-term cognitive impairments. This study found that physical exercise (PE) can protect neurons involved in memory and mitigate cognitive decline in rats post-CA. However, PE did not have a similar neuroprotective effect on hippocampal cells. The study also identified the regulation of genes related to neuroinflammation, plasticity, and cell death as potential mechanisms underlying the cognitive benefits of PE.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Yeon Joo Choi, Min Jea Shin, Gi Soo Youn, Jung Hwan Park, Hyeon Ji Yeo, Eun Ji Yeo, Hyun Jung Kwon, Lee Re Lee, Na Yeon Kim, Su Yeon Kwon, Hyo Young Jung, Yong-Jun Cho, Dae Won Kim, Jinseu Park, Kyu Hyung Han, Keun Wook Lee, Jong Kook Park, Chan Hee Lee, Won Sik Eum, Soo Young Choi
Summary: In this study, the role of GSTA2 in preventing ischemic injury was investigated using the cell-permeable PEP-1-GSTA2 protein. It was found that PEP-1-GSTA2 protected HT-22 cells from cell death induced by oxidative stress through the inhibition of ROS production and DNA damage. Additionally, PEP-1-GSTA2 promoted MAPK and NF-κB activation, and regulated the expression levels of Bcl-2, Bax, cleaved Caspase-3 and -9 proteins. In an in vivo ischemic animal model, PEP-1-GSTA2 prevented hippocampal neuron loss and reduced microglia and astrocyte activation. These findings suggest that PEP-1-GSTA2 suppresses hippocampal cell death by regulating MAPK and apoptotic signaling pathways, potentially offering a new therapeutic strategy for oxidative stress-induced ischemic injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Wenxun Liu, Qingshan Ye, Wenhua Xi, Yan Li, Xiaohong Zhou, Yun Wang, Zhenhai Ye, Kerong Hai
Summary: Heparin treatment significantly improves neurological deficits and cerebral edema in rats with CPR-induced cerebral ischemia-reperfusion injury. Heparin also reduces neuronal apoptosis and promotes neurite outgrowth in vitro. Furthermore, heparin attenuates neuronal apoptosis and neuroinflammation, potentially through regulating the ERK/CREB-mediated PTN/syndecan-3 pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Critical Care Medicine
Filippo Annoni, Fuhong Su, Lorenzo Peluso, Ilaria Lisi, Enrico Caruso, Francesca Pischiutta, Elisa Gouvea Bogossian, Bruno Garcia, Hassane Njimi, Jean-Louis Vincent, Nicolas Gaspard, Lorenzo Ferlini, Jacques Creteur, Elisa R. R. Zanier, Fabio Silvio Taccone
Summary: Prognosis after resuscitation from cardiac arrest (CA) is poor, but hypertonic sodium lactate (HSL) infusion may have beneficial effects on brain and cardiac injury. This study aimed to test the effects of HSL infusion in an experimental model of CA.
Article
Chemistry, Multidisciplinary
Anselm F. L. Schneider, Marina Kithil, M. Cristina Cardoso, Martin Lehmann, Christian P. R. Hackenberger
Summary: The challenge of delivering proteins into cells has been addressed by conjugating arginine-rich cell-penetrating peptides to the cell surface, enabling efficient delivery of protein-CPP conjugates. The use of thiol-reactive cell-penetrating peptides allows for simple co-incubation to deliver thiol-containing proteins, greatly simplifying protein and antibody delivery with minimal genetic or chemical engineering.
Article
Neurosciences
Matine Azadian, Guilian Tian, Afsheen Bazrafkan, Niki Maki, Masih Rafi, Nikole Chetty, Monica Desai, Ieeshiah Otarola, Francisco Aguirre, Shuhab M. Zaher, Ashar Khan, Yusuf Suri, Minwei Wang, Beth A. Lopour, Oswald Steward, Yama Akbari
Summary: It has been shown that overnight caloric restriction improves survival and neurological recovery in acute ischemic brain injury models, by normalizing stress-induced hyperglycemia and affecting hormone production. These benefits appear unrelated to SIRT-1 and BDNF expression, with mechanisms still needing further clarification.
FRONTIERS IN NEUROSCIENCE
(2021)
Review
Medicine, General & Internal
Rishabh C. Choudhary, Muhammad Shoaib, Samantha Sohnen, Daniel M. Rolston, Daniel Jafari, Santiago J. Miyara, Kei Hayashida, Ernesto P. Molmenti, Junhwan Kim, Lance B. Becker
Summary: Cardiac arrest causes global ischemia-reperfusion injury, leading to tissue damage throughout the body. While current non-pharmacologic therapies like therapeutic hypothermia and extracorporeal cardiopulmonary resuscitation have shown benefits in protecting against brain injury post-CA, their universal implementation is challenging. Preclinical pharmacotherapy for CA has primarily targeted singular pathways and combination therapies have yielded mixed results. Future interventions for CA should aim to target multiple pathways simultaneously to alleviate the various processes involved in brain injury.
FRONTIERS IN MEDICINE
(2021)
Review
Medicine, Research & Experimental
Ke Li, Zhu Zhu, Xiumei Sun, Linhong Zhao, Zuolong Liu, Jihong Xing
Summary: Cardiac arrest (CA) is a leading cause of death globally with a low survival rate. Restoration of spontaneous circulation (ROSC) has limited impact on mortality rate and prognosis due to ischemia-reperfusion injury. Mesenchymal stem cell (MSC)-derived exosomes have therapeutic potential in wound repair, tissue reconstruction, inflammation, and ischemic diseases by mediating intercellular communication. This review explores the prospects of using MSC-derived exosomes for the treatment of CA.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Haruya Yagi, Takumi Tomono, Yuma Handa, Natsuki Saito, Masami Ukawa, Kohei Miyata, Koichi Shigeno, Shinji Sakuma
Summary: This study investigates the use of cell-penetrating peptides anchored to polymeric platforms as an absorption enhancer for delivering biologics via mucosal routes. Results show that hyaluronic acid modified with L-octaarginine significantly enhances the mucosal absorption of protein drugs in the nasal cavities. The lung mucosa and oral mucosa were found to be suitable routes for mucosal absorption of biologics.
MOLECULAR PHARMACEUTICS
(2023)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Critical Care Medicine
Matthias Kohlhauer, Mathieu Panel, Marine Vermot des Roches, Estelle Faucher, Yara Abi Zeid Daou, Emilie Boissady, Fanny Lidouren, Bijan Ghaleh, Didier Morin, Renaud Tissier
Summary: The study found that cardiac and cerebral mitochondrial dysfunction showed similar patterns after cardiac arrest, with initial recovery within the first few hours but a significant increase in dysfunction after 24 hours. Despite greater neurological dysfunction after non-shockable cardiac arrest, mitochondrial dysfunction was still more pronounced after 24 hours.
Article
Chemistry, Multidisciplinary
Jianan Zhou, Yuting Cai, Tingting Li, Haixiang Zhou, Huilei Dong, Xia Wu, Zenghui Li, Wenjie Wang, Dan Yuan, Yun Li, Junfeng Shi
Summary: A novel topical delivery system, Gel 2_1 & Eylea, is developed using an aflibercept-loaded eye-drop hydrogel facilitated by cell-penetrating peptide 1. Gel 2_1 & Eylea demonstrates superior membrane permeability, increased stability, and prolonged drug retention time on the ocular surface, and significantly reduces the density of neovascularization with no adverse effects on normal corneoscleral limbal vessels in a rabbit CoNV model, showing high efficacy and biocompatibility. This research identifies a promising treatment for corneal neovascularization and potentially benefits other ocular neovascular diseases.
Article
Critical Care Medicine
Veerle Heesters, Janneke Dekker, Timothy J. R. Panneflek, Kristel L. A. M. Kuypers, Stuart B. Hooper, Remco Visser, Arjan B. te Pas
Summary: This study visualized the closure of vocal cords during apnea, in between breaths, and during breath holds in preterm infants using ultrasonography. The closure of vocal cords impaired the effect of respiratory support.