Article
Microbiology
Emma C. L. Marrs, Audrey Perry, John D. Perry
Summary: This study compared the efficacy of three commercially available selective media for the isolation of Burkholderia cepacia complex (BCC). The results showed that all three media were effective for BCC isolation, with BCSA being more selective than the other two media.
Article
Respiratory System
Rianald Fischer, Carsten Schwarz, Rebecca Weiser, Eshwar Mahenthiralingam, Knut Smerud, Nils Meland, Hugo Flaten, Philip Rye
Summary: This study evaluated the safety and efficacy of inhaled OligoG as a therapy for CF patients with Bcc infection. The results showed a decrease in bacterial colony-forming units after OligoG treatment, although the difference was not statistically significant. There was no significant improvement in lung function parameters, but some quality of life scores showed relative improvement. The study suggests the potential of OligoG for the treatment of Bcc infection.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Medicine, General & Internal
Dorota Wyrostkiewicz, Lucyna Opoka, Dorota Filipczak, Ewa Jankowska, Wojciech Skorupa, Ewa Augustynowicz-Kopec, Monika Szturmowicz
Summary: The aim of this study was to assess the frequency of nontuberculous mycobacteria (NTM) isolations in hospitalized cystic fibrosis (CF) patients and describe the challenges in recognizing nontuberculous mycobacterial lung disease (NTMLD) in these patients. The results showed that 7% of CF patients hospitalized for pulmonary exacerbations had positive respiratory specimen cultures for NTM. Additionally, 91% of patients had positive respiratory cultures for fungi. Due to the non-specific clinical presentation of NTMLD, frequent sputum cultures for NTM and analysis of serial chest CT examinations are crucial for its recognition in CF patients.
Article
Microbiology
Cedric Lood, Charlotte Peeters, Quentin Lamy-Besnier, Jeroen Wagemans, Daniel De Vos, Marijke Proesmans, Jean-Paul Pirnay, Fedoua Echahidi, Denis Pierard, Matthieu Thimmesch, Anca Boeras, Katrien Lagrou, Evelien De Canck, Elke De Wachter, Vera van Noort, Rob Lavigne, Peter Vandamme
Summary: The study found that Burkholderia multivorans, a pathogenic bacterium within the Burkholderia cepacia complex, primarily spreads among cystic fibrosis patients through environmental sources with rare cases of patient-to-patient transmission. Genomic analysis of an endemic strain infecting multiple patients revealed significant genomic diversity between patients, suggesting the presence of a microdiverse reservoir for this strain with active diversification. Additionally, the study identified specific genomic adaptations across multiple patients, indicating that the genomic background of a strain may dictate its route of adaptation within the cystic fibrosis lung.
Article
Medicine, General & Internal
Anna Engell Holm, Hans Henrik Lawaetz Schultz, Helle Krogh Johansen, Tania Pressler, Thomas Kromann Lund, Martin Iversen, Michael Perch
Summary: This study monitored the time of bacterial re-colonization in lung transplant patients with cystic fibrosis and found that 70% of patients were re-colonized by bacteria after 2 years, with no observed impact on survival rates from re-colonization or different bacterial species.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jerry A. Nick, Rebekah M. Dedrick, Alice L. Gray, Eszter K. Vladar, Bailey E. Smith, Krista G. Freeman, Kenneth C. Malcolm, L. Elaine Epperson, Nabeeh A. Hasan, Jo Hendrix, Kimberly Callahan, Kendra Walton, Brian Vestal, Emily Wheeler, Noel M. Rysavy, Katie Poch, Silvia Caceres, Valerie K. Lovell, Katherine B. Hisert, Vinicius Calado de Moura, Delphi Chatterjee, Prithwiraj De, Natalia Weakly, Stacey L. Martiniano, David A. Lynch, Charles L. Daley, Michael Strong, Fan Jia, Graham F. Hatfull, Rebecca M. Davidson
Summary: This study describes a successful phage treatment of treatment-refractory Mycobacterium abscessus pulmonary infection in a patient with severe lung disease. The phages used in the treatment were engineered to enhance their ability to lyse M. abscessus and were specifically selected based on their effectiveness against the patient's bacterial isolate. The treatment resulted in genetic stability of the bacterial isolate and improved clinical symptoms in the patient.
Article
Infectious Diseases
Dustin Maydaniuk, Bin Wu, Dang Truong, Sajani H. Liyanage, Andrew M. Hogan, Zhong Ling Yap, Mingdi Yan, Silvia T. Cardona
Summary: Bacteria of the genus Burkholderia, including pathogenic species, show intrinsic drug resistance. Auranofin analogs such as MS-40 and MS-40S exhibit antibiotic activity against Burkholderia clinical isolates, killing stationary-phase cells without inducing multistep resistance, and demonstrating low toxicity in model organisms. Further investigations are needed to explore the therapeutic potential of these compounds against Burkholderia infections.
Article
Infectious Diseases
Frieder Schaumburg, Evgeny A. Idelevich, Alexander Mellmann, Barbara C. Kahl
Summary: Burkholderia cepacia complex (Bcc) in the airways of cystic fibrosis (CF) patients is associated with higher morbidity and mortality. This study assessed the susceptibility of Bcc to ceftazidime/avibactam and other standard drugs used for CF treatment, as well as identified the genomic determinants of resistance. The results showed that Bcc had a high susceptibility rate to ceftazidime/avibactam, while being less susceptible to other antibiotics.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Respiratory System
Christina Thornton, Ranjani Somayaji, Angel Chu, Michael D. Parkins
Summary: Human papillomavirus (HPV) is a major risk factor for cervical cancer, and transplant recipients have a higher risk of HPV complications. A retrospective study on adult female cystic fibrosis (CF) lung transplant recipients found that 35.3% had abnormal pap smear results, including refractory anogenital warts, vulvectomy, and cervical cancer. The study also revealed that lung transplant recipients had a higher likelihood of cervical dysplasia compared to the control group, emphasizing the importance of HPV vaccination to prevent future morbidity and mortality.
Article
Anesthesiology
Julien Fessler, Matthieu Glorion, Michael Finet, Claire Soniak, Sylvie Colin de Verdiere, Edouard Sage, Antoine Roux, Benjamin Zuber, Marc Fischler, Morgan Le Guen, Alexandre Vallee
Summary: This retrospective study compared the intraoperative and early postoperative features between patients with primary double-lung transplantation (pLgTx) and retransplantation (reLgTx) for cystic fibrosis (CF). The study found that retransplantation was more complex but the early postoperative period was similar to primary transplantation.
JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA
(2023)
Article
Microbiology
Amy Pham, James G. Volmer, Daniel C. Chambers, Daniel J. Smith, David W. Reid, Lucy Burr, Timothy J. Wells
Summary: In this study, we genomically characterized Burkholderia anthina isolated from patients with chronic lung infections, which revealed two distinct evolutionary lineages and demonstrated substantial metabolic diversity, including enhanced capability for iron metabolism.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Angela Koutsokera, Jenna Sykes, M. Math, Olga Theou, Kenneth Rockwood, Carolin Steinack, Marie-France Derkenne, Christian Benden, Thorsten Krueger, Cecilia Chaparro, John-David Aubert, Paola Soccal Gasche, Christophe Von Garnier, Elizabeth Tullis, Anne L. Stephenson, Lianne G. Singer
Summary: This study developed a CF-specific frailty index and found that it was significantly associated with wait-list and post-LT outcomes in CF patients listed for lung transplantation.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Article
Immunology
Edgard de Freitas Vianna, Nicoli Izzy Miotto Menezes, Mila Muraro de Almeida, Fla'via Alvim Dutra de Freitas, Cassiana da Costa Ferreira Leite, Monica Cassia Firmida, Davi Ribeiro Iglesias de Medeiros, Robson Souza Leao, Rodolpho Mattos Albano, Elizabeth Andrade Marques
Summary: The genome analysis of Burkholderia cenocepacia complex III revealed high virulence and antimicrobial resistance, with a predominance of genes related to invasion mechanisms and flagellar biosynthesis. Additionally, the isolate exhibited bacterial dissemination mechanisms with invasive determinants.
Article
Microbiology
Holly K. Huse, Mark J. Lee, Mandy Wootton, Susan E. Sharp, Maria Traczewski, John J. LiPuma, Peter Jorth
Summary: This study evaluated the reproducibility and accuracy of different antimicrobial susceptibility testing methods for Burkholderia cepacia complex (BCC). The findings showed poor accuracy for disk diffusion and MicroScan WalkAway methods, while broth microdilution method demonstrated higher reproducibility for meropenem and minocycline.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Shekooh Behroozian, James E. A. Zlosnik, Wanjing Xu, Loretta Y. Y. Li, Julian E. Davies
Summary: There is a crisis in healthcare due to antibiotic-resistant bacteria, necessitating the search for novel antimicrobial agents. Kisameet clay has been found to inhibit acute infections, presenting a potential therapy. This offers hope for addressing the challenge of chronic bacterial infections and improving treatment options.