Review
Infectious Diseases
Dana Holger, Razieh Kebriaei, Taylor Morrisette, Katherine Lev, Jose Alexander, Michael Rybak
Summary: Pseudomonas aeruginosa is a common pathogen causing healthcare-associated diseases and is highly resistant to many antibiotics, posing challenges in treatment. Bacteriophages, as viruses targeting and infecting bacterial cells, offer promise as an alternative therapy for multidrug-resistant P. aeruginosa infections. Further studies on phage pharmacokinetics and pharmacodynamics are needed to guide successful phage therapy for these infections.
Article
Microbiology
Luke Diorio-Toth, Sidra Irum, Robert F. Potter, Meghan A. Wallace, Muhammad Arslan, Tehmina Munir, Saadia Andleeb, Carey-Ann D. Burnham, Gautam Dantas
Summary: This study used whole-genome sequencing to determine the genotypic mechanisms of beta-lactam resistance in Pseudomonas aeruginosa clinical isolates from three hospitals in Pakistan. The study found that carbapenem resistance was associated with the acquisition of metallo-beta-lactamases or extended-spectrum beta-lactamases and mutations in the porin gene. Furthermore, isolates with multiple resistance mechanisms showed significantly higher levels of imipenem resistance compared to isolates with a single resistance mechanism.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Vaughn D. Craddock, Evan L. Steere, Hannah Harman, Nicholas S. Britt
Summary: Delafloxacin (DLX) is a newly approved fluoroquinolone that shows broad activity against common cystic fibrosis (CF) pathogens, including multidrug-resistant Pseudomonas aeruginosa (MDR-Psa). DLX exhibits excellent penetration in the CF lung and has enhanced activity in low pH environments. In this study, DLX was compared to ciprofloxacin (CPX) and levofloxacin (LVX) against Psa strains isolated from CF sputum. DLX showed significant improvements in killing bacteria in a CF sputum time-kill model at physiologically relevant drug concentrations, making it a potential option for treating MDR-Psa pulmonary infections in CF patients.
Article
Virology
Ismahen Akremi, Maya Merabishvili, Mouna Jlidi, Adel Haj Brahim, Manel Ben Ali, Anis Karoui, Rob Lavigne, Jeroen Wagemans, Jean-Paul Pirnay, Mamdouh Ben Ali
Summary: This study isolated and identified four lytic Pseudomonas aeruginosa bacteriophages, which showed activity against a wide range of P. aeruginosa strains under various temperature and pH conditions. The phages had high sequence identity to Pseudomonas phage PAK_P1 and were assigned to the Pakpunavirus genus. This research contributes to establishing phage therapy as an alternative strategy for managing multidrug-resistant P. aeruginosa infections in Tunisia.
Article
Microbiology
Haley J. Appaneal, Aisling R. Caffrey, Vrishali Lopes, Emily C. Piehl, Laura A. Puzniak, Kerry L. LaPlante
Summary: We studied antibiotic co-resistance patterns in a national group of hospitalized patients with multidrug-resistant Pseudomonas aeruginosa infections. Co-resistance to commonly used antibiotic classes was common, making it difficult to select appropriate antibiotics for treatment. Poor outcomes were observed for all patients, regardless of different patterns of co-resistance. New antibiotics are needed to help treat hospitalized patients with multidrug-resistant P. aeruginosa infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Agnieszka Litwin, Stanislaw Rojek, Waldemar Gozdzik, Wieslawa Duszynska
Summary: The study analyzed the data of device associated- healthcare associated infections in an ICU, revealing fluctuating prevalence of P. aeruginosa infections over 8.5 years, with a significant decrease in 2016 and an increase in 2019. This highlights the importance of continuous monitoring and control measures in hospital infection control and antibiotic policy.
BMC INFECTIOUS DISEASES
(2021)
Review
Environmental Sciences
Prabin Dawadi, Christina Khadka, Manita Shyaula, Gopiram Syangtan, Tista Prasai Joshi, Samantha H. Pepper, Sushil R. Kanel, Lok R. Pokhrel
Summary: This study provides a quantitative estimate of metallo-beta-lactamases (MBLs) producing Pseudomonas aeruginosa isolates in Nepalese patients and determines the correlation between MBLs and multi-drug resistance (MDR). The meta-analysis of 19 selected studies reveals a prevalence of 14% for MBL-PA and 42% for MDR isolates in Nepal. These findings emphasize the importance of closely monitoring antibiotic prescription and exploring alternative therapeutic modalities against MBL-PA.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Lopamudra Kher, Domenico Santoro, Karen Kelley, Daniel Gibson, Gregory Schultz
Summary: Nanosulfur exhibits antimicrobial and anti-biofilm activity against multidrug-resistant Staphylococcus pseudintermedius, while being ineffective against multidrug-resistant Pseudomonas aeruginosa. Nanosulfur has the potential to be used as an alternative to antimicrobials and anti-biofilm agents in veterinary and human medicine.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Remi Le Guern, Teddy Grandjean, Sarah Stabler, Marvin Bauduin, Philippe Gosset, Eric Kipnis, Rodrigue Dessein
Summary: Gut microbiome dysbiosis caused by carbapenemase-producing Enterobacterales (CPE) colonisation is associated with worsened outcomes of Pseudomonas aeruginosa lung infection in mice. The dysbiosis is characterised by decreased alveolar macrophages and conventional dendritic cells, as well as a decrease in the Muribaculaceae family and lower levels of short-chain fatty acids. Faecal microbiota transplantation restores immune responses and lung infection outcomes, indicating the involvement of CPE-induced gut dysbiosis in altering the gut-lung immune axis.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Justyna W. Adamiak, Varsha Jhawar, Vincent Bonifay, Courtney E. Chandler, Inga Leus, Robert K. Ernst, Herbert P. Schweizer, Helen Zgurskaya
Summary: The study analyzed the adaptive response of P. aeruginosa lacking RND-mediated efflux activities, revealing significant changes in several key pathways to protect the cell envelope. The findings demonstrate that the cells accumulate PQS signals triggering iron starvation and activation of siderophore biosynthesis, leading to lipid A modification and membrane protection through a transcriptionally regulated RND pump.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Anthony Sophonsri, Corey Kelsom, Mimi Lou, Paul Nieberg, Annie Wong-Beringer
Summary: The study found that patients coinfected with CRKP and another carbapenem-resistant pathogen have more significant morbidity and require longer hospital stays compared to those with CRKP monoinfection. Empiric therapy targeting both CRKP and carbapenem-resistant Pseudomonas aeruginosa may be beneficial for at-risk patients with pneumonia.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Jin Suk Kang, Chisook Moon, Seok Jun Mun, Jeong Eun Lee, Soon Ok Lee, Shinwon Lee, Sun Hee Lee
Summary: The study retrospectively reviewed the medical records of patients with Pseudomonas aeruginosa bacteremia admitted to a tertiary hospital in Korea from 2009 to 2020, analyzing changes in antimicrobial susceptibility and risk factors for antimicrobial resistance. The results showed increasing rates of carbapenem-resistant P. aeruginosa, multidrug-resistant P. aeruginosa, and extensively drug-resistant P. aeruginosa, which were associated with discordant empirical antimicrobial therapy.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2021)
Review
Microbiology
Mireia Lopez-Siles, Andres Corral-Lugo, Michael J. McConnell
Summary: Antimicrobial resistance poses a major threat to global public health, and vaccines have the potential to reduce the burden of multidrug resistant Gram-negative bacterial infections. However, technical, logistical and societal hurdles have hindered successful vaccine development. This review discusses past efforts, current advances, and future directions in the development of vaccines for these problematic pathogens.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Immunology
Wusheng Deng, Yanmei Lei, Xiujia Tang, Dingbin Li, Jinhua Liang, Jing Luo, Liuyuan Liu, Wenshu Zhang, Liumei Ye, Jinliang Kong, Ke Wang, Zhaoyan Chen
Summary: This study investigated whether DNase I can inhibit the early formation of biofilms in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. The results showed that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting bacterial growth. Therefore, DNase I has therapeutic potential against empyema biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Silvia Gervasoni, Jitender Mehla, Charles R. Bergen, Inga V. Leus, Enrico Margiotta, Giuliano Malloci, Andrea Bosin, Attilio V. Vargiu, Olga Lomovskaya, Valentin V. Rybenkov, Paolo Ruggerone, Helen I. Zgurskaya
Summary: This study investigated the dynamics of MexB interactions with its ligands using machine learning, computational and experimental approaches. The results showed that MexB ligands can be effectively expelled or hindered in the transporter cycle based on their interactions with specific residues in MexB binding sites. The findings indicate that MexB ligands fit into a substrate-inhibitor spectrum depending on their physico-chemical properties. This molecular-level information is important for the rational design of new inhibitors and antibiotics less susceptible to the efflux mechanism.
Article
Biochemistry & Molecular Biology
Georgios Kassianidis, Athanasios Siampanos, Garyphalia Poulakou, George Adamis, Aggeliki Rapti, Haralampos Milionis, George N. Dalekos, Vasileios Petrakis, Styliani Sympardi, Symeon Metallidis, Zoi Alexiou, Theologia Gkavogianni, Evangelos J. Giamarellos-Bourboulis, Theoharis C. Theoharides
Summary: This study investigates the trajectory of moderate and severe COVID-19 progressing to ARDS requiring MV. The levels of various mediators were analyzed in patients at different stages of severity. The study found that levels of proinflammatory mediators were increased in patients with ARDS and MV, while levels of anti-inflammatory mediators also showed an increase. Pathways involving IL-6, IL-33, and calprotectin were significantly associated with worse outcomes. Further research is needed to understand the importance of these findings in relation to other acute-phase reactants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Dermatology
Evangelos J. Giamarellos-Bourboulis
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Review
Microbiology
Georgios Tziatzios, Paraskevas Gkolfakis, Gabriela Leite, Ruchi Mathur, Georgia Damoraki, Evangelos J. J. Giamarellos-Bourboulis, Konstantinos Triantafyllou
Summary: Functional dyspepsia (FD) is a common disorder with no definitive, universal treatment. Probiotics have been suggested as a potential therapeutic option for FD due to their effects on gastrointestinal symptoms in patients with Irritable Bowel Syndrome (IBS). However, the evidence for the efficacy of probiotics in FD remains unclear. This review aims to summarize the available evidence for the use of probiotics in FD.
Article
Hematology
Maria Tsilika, John Mitrou, Nikolaos Antonakos, Ioulia K. Tseti, Georgia Damoraki, Konstantinos Leventogiannis, Evangelos J. Giamarellos-Bourboulis
Summary: The clinical trial of the new oral iron formulation, Fe-ASP, demonstrates its non-inferiority to ferrous sulfate in increasing hemoglobin levels and improving global score and reducing iron-deficiency anemia-related physical signs.
ANNALS OF HEMATOLOGY
(2023)
Article
Infectious Diseases
Theodossis Papavramidis, Ivan Gentile, Anna Maria Cattelan, Laura Magnasco, Pierluigi Viale, Daniela Francisci, Diamantis P. Kofteridis, Giusy Tiseo, Evangelos J. Giamarellos-Bourboulis, Filippo Lagi, Simone Mornese Pinna, Federico D'Amico, Lucia La Ferla, Periklis Panagopoulos, Gianni Gattuso, Nikolaos V. Sipsas, Alessandro Ruggieri, Agnese Cattaneo, Luciana Corio, Alessandro Comandini, Patrizia Mascagni, Matteo Bassetti
Summary: A multicentre, observational, retrospective study showed that dalbavancin can significantly reduce hospital stay, decrease healthcare costs, and improve treatment efficacy and safety for patients with acute bacterial skin and skin-structure infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Biochemistry & Molecular Biology
Tobias Hoelle, Patrick Rehn, Konstantinos Leventogiannis, Antigone Kotsaki, Theodora Kanni, Nikolaos Antonakos, Christos Psarrakis, Georgia Damoraki, Judith Schenz, Felix C. F. Schmitt, Florian Uhle, Markus A. Weigand, Evangelos J. Giamarellos-Bourboulis, Maximilian Dietrich
Summary: This study evaluated the potential of Host-Derived Delta-like Canonical Notch Ligand 1 (DLL1) as a biomarker for diagnosing sepsis. Results showed that plasma levels of soluble DLL1 were significantly elevated in patients with sepsis compared to those with uncomplicated infections and sterile inflammation. DLL1 demonstrated better diagnostic performance for sepsis compared to other biomarkers such as C-reactive protein, procalcitonin, and White Blood Cell count.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Dermatology
Dimitra Stergianou, Styliani Micha, Vassiliki Tzanetakou, Theodora Kanni, Theologia Gkavogianni, Alexandros Katoulis, Evangelos J. Giamarellos-Bourboulis
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Francesco Vladimiro Segala, Emanuele Rando, Federica J. Salvati, Marcantonio Negri, Francesca Catania, Flavia Sanmartin, Rita Murri, Evangelos Giamarellos-Bourboulis, Massimo Fantoni
Summary: A retrospective cohort study found that early administration of anakinra, an interleukin-1 receptor antagonist, guided by baseline suPAR levels, can prevent disease progression and death in COVID-19 patients. In the absence of suPAR testing, the SCOPE score can be used as an alternative for treatment decisions.
Article
Health Care Sciences & Services
Stavroula Zanelli, Agamemnon Bakakos, Zoi Sotiropoulou, Andriana I. Papaioannou, Evangelia Koukaki, Efstathia Potamianou, Anna Kyriakoudi, Evangelos Kaniaris, Petros Bakakos, Evangelos J. Giamarellos-Bourboulis, Antonia Koutsoukou, Nikoletta Rovina
Summary: This study assessed the modified Severe COvid Prediction Estimate score (mSCOPE) as a predictor of mortality in ICU-admitted severe COVID-19 pneumonia patients. The results showed that patients with higher mSCOPE scores had a higher mortality rate and were correlated with disease severity, comorbidity severity, days on mechanical ventilation, and days of ICU stay. The mSCOPE score can be a useful tool for risk stratification in patients with severe COVID-19.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Editorial Material
Dermatology
Evangelos J. Giamarellos-Bourboulis
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Article
Pathology
Evdoxia Kyriazopoulou, Evangelos J. Giamarellos-Bourboulis, Karolina Akinosoglou
Summary: This review summarizes the current progress in the development of biomarkers to guide immunotherapy in oncology, rheumatology, and critical illness.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Correction
Critical Care Medicine
Evangelos J. Giamarellos-Bourboulis, Annelies S. Zinkernagel, Edoardo De Robertis, Elie Azoulay, Daniele De Luca
INTENSIVE CARE MEDICINE
(2023)
Letter
Critical Care Medicine
Evangelos J. Giamarellos-Bourboulis, Annelies S. Zinkernagel, Edoardo De Robertis, Elie Azoulay, Daniele De Luca
INTENSIVE CARE MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Nick Keur, Maria Saridaki, Isis Ricano-Ponce, Mihai G. Netea, Evangelos J. Giamarellos-Bourboulis, Vinod Kumar
Summary: This study identified protein biomarkers associated with COVID-19 severity through the measurement of plasma protein levels. It also identified three clinical endotypes of the disease. These findings suggest that circulating proteins may be useful for predicting COVID-19 outcomes and may be applicable to other populations.
RESPIRATORY MEDICINE
(2023)
Article
Multidisciplinary Sciences
Nicky Solomonidi, Panayiotis G. Vlachoyiannopoulos, Maria Pappa, Georgia Liantinioti, Sofia Ktena, Evangelos Theotikos, Antonia Elezoglou, Mihai G. Netea, Evangelos J. Giamarellos-Bourboulis
Summary: This study found that increased concentrations of IL-1 alpha are present in SSc patients. Treatment with the IL-1 alpha blocking antibody, bermekimab, effectively inhibits disease progression and reduces inflammation.