Journal
RESEARCH IN VETERINARY SCIENCE
Volume 97, Issue 2, Pages 325-328Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2014.06.015
Keywords
Prevalence; PCR; Hepatozoonosis; Dogs; Brazil
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Funding
- Sao Paulo State University
- FAPEMIG
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The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlandia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlandia and that infection is similarly widespread in rural and urban dogs. (C) 2014 Published by Elsevier Ltd.
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