Article
Biochemistry & Molecular Biology
Yurong Fu, Yongcheng Jin, Yue Tian, Hao Yu, Ruqi Wang, Huiyu Qi, Bo Feng, Jing Zhang
Summary: The synergistic effect of ZEA and LPS aggravates cytotoxicity by increasing the accumulation of ROS and MDA, decreasing MMP and SOD levels, and reducing GSH levels. Additionally, ZEA and LPS promote cell apoptosis by activating ER stress and suppressing anti-apoptotic proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Petr Slama, Terezie Zavadilova, Ales Pavlik, Pavel Horky, Sylvie Skalickova, Jiri Skladanka, Shubhadeep Roychoudhury, Simona Baldovska, Adriana Kolesarova, Roman Konecny, Vladimir Tancin, Monika Zouharova
Summary: Bovine mastitis is a significant issue for dairy farmers with antibiotic treatment needing to be minimized due to resistance. Streptococcus uberis is a common pathogen, with research indicating that gamma delta T cells may play a key role in resolving inflammation in mammary glands.
Article
Agriculture, Dairy & Animal Science
Lei Liu, Ouyang Lu, Dan Li, Yuan Tian, Ziling Liu, Yanqiong Wen, Tao Peng, Yuxiang Song, Xiliang Du, Zhe Wang, Guowen Liu, Xinwei Li
Summary: Ketosis in dairy cows is associated with increased apoptosis in mammary glands due to systemic oxidative stress. SIRT3 mitigates oxidative stress-induced apoptosis in mammary gland cells through AMPK signaling.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: This study aimed to investigate the harmful effects of folpet on bovine mammary glands and milk production using MAC-T cells. The results showed that folpet exhibited cytotoxicity against MAC-T cells, causing apoptosis and cell death. Furthermore, folpet treatment induced oxidative stress and activated MAPK signaling pathways. This is the first report highlighting the detrimental impacts of folpet on bovine mammary glands and the dairy industry, providing insights into the intracellular mechanisms using MAC-T cells.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gerald Cohen
Summary: This study investigated the effect of HDL from CKD and HD patients on CD14 expression on PMNLs. The findings showed that HDL increased CD14 surface expression on PMNLs, with a more significant effect seen with HDL from uremic patients. SAA as part of an HDL particle did not increase CD14 expression, and disruption of lipid rafts led to reduced CD14 expression. HDL from healthy subjects decreased Rac1 activity, while HDL from HD patients did not.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Peter Michael Reil, Teodor Traian Maghiar, Narcis Vilceanu, Andrei Pascalau, Claudia Teodora Judea Pusta, Florin Marcu, Simona Cavalu, Ovidiu Pop
Summary: The study found that in patients who died of septic shock, the expression levels of CD14 in heart tissue were correlated with adverse patient outcomes, indicating that CD14 could be a valuable marker for septic cardiomyopathy.
Article
Biochemistry & Molecular Biology
Magdalena Keller, Elisa Manzocchi, Deborah Rentsch, Rosamaria Lugara, Katrin Giller
Summary: Replacing soybean meal with spirulina in the diets of dairy cows and fattening bulls did not show significant antioxidant and anti-inflammatory effects in ruminants. Future studies should focus on evaluating the microbial degradation of spirulina and its bioactive compounds in the rumen to provide more data on potential beneficial health effects in ruminants.
Article
Agriculture, Dairy & Animal Science
Denis Revskij, Susanne Haubold, Christian Plinski, Torsten Viergutz, Armin Tuchscherer, Claudia Kroeger-Koch, Elke Albrecht, Juliane Guenther, Arnulf Troescher, Harald M. Hammon, Hans-Joachim Schuberth, Manfred Mielenz
Summary: The study found that CXCR4 is clearly expressed in the ductal epithelial cells of the mammary gland but not in alveolar epithelial cells. Among leukocyte subsets, monocytes displayed the highest percentage of CXCR4-positive cells, while circulating neutrophils showed almost no CXCR4 surface expression.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zaneta Dziegelewska-Sokolowska, Alicja Majewska, Adam Prostek, Malgorzata Gajewska
Summary: The mammary gland consists of epithelial tissue and stroma, with preadipocytes and adipocytes as important components of the stroma. This study investigated the effects of paracrine factors secreted by preadipocytes and adipocytes on the development of bovine mammary epithelial cells. The results showed that factors secreted by fully differentiated adipocytes and preadipocytes can decrease apoptosis and increase the size of spheroids formed by the mammary epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Wei Lan, Yifei Ren, Zhen Wang, Jianxin Liu, Hongyun Liu
Summary: The study demonstrates that methionyl-methionine alleviates inflammation in bovine mammary epithelial cells by altering processes of amino acid, purine, and fatty acid metabolism.
Article
Cell Biology
Lu Ouyang, Huilun Tang, Zilin Liu, Yuan Tian, Xin Gao, Tao Peng, Zuo Wang, Xinyi Lan, Weijun Shen, Dingfu Xiao, Fachun Wan, Lei Liu
Summary: Resveratrol alleviated lipopolysaccharide-induced apoptosis in bovine mammary epithelial cells through the PGC1 alpha-SIRT3 axis, suggesting its potential application in relieving mastitis.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2023)
Article
Multidisciplinary Sciences
Doreen Becker, Rosemarie Weikard, Frieder Hadlich, Christa Kuehn
Summary: The analysis of single-cell RNA datasets from bovine milk cells and pbMECs revealed distinct clusters within each population, indicating heterogeneity between the populations. These datasets provide a molecular cell atlas for future studies of milk cell composition and gene expression.
Article
Cell Biology
Yue Zhang, Qiong Wu, Guanglin Niu, Jidan Liu, Fangjun Cao, Xiaopeng An, Binyun Cao
Summary: This study found that EGF can regulate apoptosis and inflammation in gMEC by inducing the expression of miR-223. The downregulation of ISG15 by EGF and miR-223 is a key factor in this regulatory process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hakyoung You, Garam An, Hojun Lee, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the toxic effects of bifenox on bovine mammary glands using MAC-T cells. Our findings showed that bifenox inhibited cell proliferation, disrupted the cell cycle, and caused disturbances in calcium homeostasis and mitochondrial membrane potential. We also observed hyperactivation of PI3K/AKT and MAPK signaling cascades in response to bifenox treatment.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Cell Biology
Shalini Jaswal, Manoj Kumar Jena, Vijay Anand, Avinash Jaswal, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty
Summary: The mammary gland undergoes repeated cyclic changes throughout the life of mammals. Cattle and buffalo are major milk-producing livestock species that play essential roles in farming communities. The development of the ruminant mammary gland is highly dynamic and involves multiple stages. However, the molecular processes underlying cow udder development have not been thoroughly investigated. This review focuses on the morphological and molecular changes during various stages of cow udder development, providing insights into the physiological differences between cattle and other mammalian species.
Article
Agriculture, Dairy & Animal Science
Anna Kubesova, Kamil Stastny, Martin Faldyna, Zbysek Sladek, Iva Steinhauserova, Gabriela Borilova, Ales Knoll
CZECH JOURNAL OF ANIMAL SCIENCE
(2019)
Article
Agriculture, Dairy & Animal Science
Petr Slama, Eliska Kabourkova, Zbysek Sladek, Terezie Zavadilova, Lucie Kratochvilova, Kristina Kharkevich, Shubhadeep Roychoudhury, Ales Pavlik, Andrea Roztocilova, Michal Uhrincat, Vladimir Tancin, Kazuhiro Kimura, Roman Konecny, Yoshio Kiku, Atsushi Watanabe, Jong-Young Kwak, Monika Zouharova
Article
Biochemistry & Molecular Biology
Tomas Komprda, Zbysek Sladek, Zuzana Sevcikova, Veronika Svehlova, Jan Wijacki, Roman Guran, Tomas Do, Zuzana Lackova, Hana Polanska, Lucie Vrlikova, Vendula Popelkova, Petr Michalek, Ondrej Zitka, Marcela Buchtova
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Tomas Do, Roman Guran, Rea Jarosova, Petra Ondrackova, Zbysek Sladek, Martin Faldyna, Vojtech Adam, Ondrej Zitka
Article
Biochemistry & Molecular Biology
Tomas Komprda, Zbysek Sladek, Monika Vicenova, Jana Simonova, Gabriela Franke, Katarina Kacvinska, Cristina Sabliov, Carlos E. Astete, Lenka Leva, Vendula Popelkova, Andrej Batik, Lucy Vojtova, Bretislav Lipovy, Milena Matejovicova
Summary: The utilization of PLGA nanoparticles with entrapped fish oil in collagen-based scaffolds for cutaneous wound healing showed promising results in a porcine model. The NP/FO treatment had advantages compared to FO alone and tended to have similar effects to NP/MUP treatment, suggesting it as a suitable alternative for wound treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Agriculture, Dairy & Animal Science
Kristyna Skoupa, Kamil St'astny, Zbysek Sladek
Summary: Anabolic steroids have significant effects on animal tissues and can cause irreversible morphological and histological changes. The use of these steroids in animal fattening raises concerns about consumer health and there is a need for new detection methods. The abuse of anabolic steroids can result in various side effects, including organ damage, brain atrophy, and behavioral changes. While the use of steroids is permitted in many countries, it is strictly banned in all European Union countries. Serum analysis and tissue structure changes may serve as potential new methods for detecting steroid misuse.
Article
Veterinary Sciences
Rea Jarosova, Petra Ondrackova, Lenka Leva, Katerina Nedbalcova, Monika Vicenova, Josef Masek, Jiri Volf, Jan Gebauer, Tomas Do, Roman Guran, Zbysek Sladek, Javier Dominguez, Martin Faldyna
Summary: CD163+ monocytes contribute to the production of inflammatory cytokines during APP-induced lung inflammation, especially IL-1 beta, at the site of inflammation. PB and BM CD163+ monocytes do not appear to play a significant role in elevated cytokine levels in plasma.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Rea Jarosova, Juliette Ben Arous, Katerina Nechvatalova, Katerina Nedbalcova, Karolina Hlavova, Hana Stepanova, Lenka Leva, Zrinka Oreskovic, Jan Matiasovic, Nicolas Versille, Zbysek Sladek, Martin Faldyna
Summary: The route of vaccine delivery significantly affects its immunogenicity, efficacy, and safety. In this study, different routes of delivery were compared in piglets, and it was found that intramuscular administration showed the highest efficacy while dermal administration was slightly less immunogenic. Interestingly, dermal administration provided superior protection effectiveness compared to other routes.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kristyna Skoupa, Andrej Batik, Kamil St'astny, Zbysek Sladek
Summary: This study investigates the effects of anabolic steroids on pig muscle tissue, finding that they increase the size and surface area of muscle fibers, as well as significantly increasing the number of satellite cells. These findings suggest that histological parameters could be used as additional detection mechanisms for screening a large number of samples and indicate the potential presence of anabolic steroids in pork meat in the future.
Article
Veterinary Sciences
Rea Jarosova, Petra Ondrackova, Zdenek Patocka, Zbysek Sladek
Summary: Proper histological evaluation of lung tissue and accurate antigen detection by immunodetection techniques require appropriate tissue processing methods. This study tested and compared various tissue cryopreservation procedures, ultimately finding the optimal approach to preserve tissue reactivity and enable proper anatomical evaluation.
ACTA VETERINARIA BRNO
(2021)
Proceedings Paper
Agriculture, Multidisciplinary
Tomas Do, Rea Jarosova, Lada Ilieva, Jiri Pospisil, Roman Guran, Petra Ondrackova, Martin Faldyna, Zbysek Sladek, Ondrej Zitka
MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE
(2019)
Proceedings Paper
Agriculture, Multidisciplinary
Tomas Do, Rea Jarosova, Eliska Sedlackova, Roman Guran, Petra Ondrackova, Martin Faldyna, Zbysek Sladek, Ondrej Zitka
MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE
(2019)
Proceedings Paper
Agriculture, Multidisciplinary
Rea Jarosova, Tomas Do, Barbora Tesarova, Veronika Smidova, Roman Guran, Petra Ondrackova, Martin Faldyna, Zbysek Sladek, Ondrej Zitka
MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE
(2019)
Article
Veterinary Sciences
Tomas Komprda, Zbysek Sladek, Veronika Svehlova, Zuzana Lackova, Roman Guran, Tomas Do, Jan Wijacki, Marcela Buchtova, Jana Neuwirthova, Bretislav Gal, Vojtech Adam, Ondrej Zitka
ACTA VETERINARIA BRNO
(2019)
Article
Food Science & Technology
Terezie Zavadilova, Zbysek Sladek, Lucie Kratochvilova, Petr Slama, Dusan Rysanek
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2019)
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)
