Journal
RESEARCH IN VETERINARY SCIENCE
Volume 88, Issue 1, Pages 83-87Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2009.05.021
Keywords
Cephalosporin resistance; Escherichia coli; Healthy pigs; Farm-level; CTX-M; SHV-12
Categories
Funding
- Spanish Ministerio de Educacion y Ciencia [AGL2002-02637, SAF2006-14207-C02]
- Programa Juan de la Cierva [CTE/351/2004]
- MED-VET-NET [WP 21]
- Consejeria de Educacion - Comunidad de Madrid (Spain) [S-0505/AGR/000265]
- Spanish Ministerio de Medio Ambiente y Medio Rural y Marino
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Introduction: Fecal Escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in Spain. The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level. Materials and methods: Twenty-nine E. coli isolates showing reduced susceptibility or resistance to extended-spectrum cephalosporins were collected from a sampling frame of 80 pig farms distributed over 13 Spanish provinces. The survey was carried out at the slaughterhouse level in 2004. Results: Of the 29 isolates, 21 (72%) met the criteria for a positive phenotypic confirmatory test for extended-spectrum beta-lactamases (ESBL). The following ESBLs were detected: SHV-12 (12 isolates, 41%), CTX-M-1 (three isolates, 10%), CTX-M-9 (three isolates, 10%), and CTX-M-14 (three isolates, 10%). The remaining eight isolates (28%) were phenotypically non-ESBL, with seven of them (24%) showing mutations on the chromosomal ampC gene promoter at positions -42 (C -> T), -18 (G -> A), -1 (C -> T), and +58 (C -> T). A multiplex PCR for detection of plasmidic class C beta-lactamases was negative for all isolates. Conclusion: Different ESBLs and other mechanisms linked to extended-spectrum cephalosporin resistance are widely distributed among fecal E. coli from slaughter pigs in Spain. (C) 2009 Elsevier Ltd. All rights reserved.
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