Article
Parasitology
Yuan-Yuan Cao, Shu-Wen Xiao, Feng Yang, Xiao-Ya Liu, Hui Lu, Jin-Cheng Zhang, Yong-Hong Hu
Summary: In this study, the recombinant protein of Fructose1,6-bisphosphate aldolase (FBA) from Haemaphysalis longicornis was obtained and found to be immunogenic against ticks. Immunized rabbits produced a specific humoral immune response to the recombinant FBA protein. Tick infestation trial showed that the engorged tick weight, oviposition, and egg hatching rate were significantly reduced in the group vaccinated with FBA recombinant protein. The overall immune efficacy of FBA was estimated to be 68.4%.
PARASITES & VECTORS
(2023)
Article
Biochemistry & Molecular Biology
Jikun Xia, Wanmeng Xin, Fang Wang, Wancui Xie, Yi Liu, Jiakun Xu
Summary: Fructose-1,6-bisphosphate aldolase is a conserved enzyme involved in glycolysis and gluconeogenesis. In this study, the gene encoding aldolase was cloned from Euphausia superba and expressed in Escherichia coli. The purified protein showed high homology to aldolases from other species. It exhibited optimal activity at 45 degrees C and pH 7.0 and was sensitive to the presence of borohydride. Specific amino acid residues were identified as key for the enzyme's functionality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bingbing Cai, Yu Ning, Qiang Li, Qingyun Li, Xizhen Ai
Summary: This study reveals the important role of the SlFBA4 gene in tomato growth and chilling tolerance. Over-expression of SlFBA4 increases photosynthesis and enzyme activity, leading to improved plant growth. Conversely, RNAi suppression of SlFBA4 results in opposite effects. Furthermore, over-expression of SlFBA4 enhances photosynthesis and enzyme activity, while reducing oxidative stress under chilling conditions. In contrast, RNAi lines exhibit more severe impairments in photosynthesis and oxidative stress under chilling stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Xuedong He, Jing Zhang, Yue Sun, Tianyan Lan, Xiaola Guo, Xiaoqiang Wang, Omnia M. Kandil, Mazhar Ayaz, Xuenong Luo, Houhui Song, Yadong Zheng
Summary: Glycolysis is an important energy-acquiring pathway in Echinococcus multilocularis. Fructose-1, 6-bisphosphate aldolase (FBA) plays a crucial role in this process. Genome-wide analysis revealed the presence of four fba genes (FBA1-4) in Echinococcus species, with EmFBA1 being mainly expressed in the germinal layer and posterior of the protoscolex. EmFBA1 enzyme activity is susceptible to Fe3+ and significantly affected in its presence.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
John N. Koberstein, Melissa L. Stewart, Chadwick B. Smith, Andrei Tarasov, Frances M. Ashcroft, Philip J. S. Stork, Richard H. Goodman
Summary: Cellular metabolism is regulated to ensure efficient energy production and consumption. Fluorescent biosensors enable real-time detection of metabolite abundance with single-cell resolution. Researchers have developed a new biosensor called HYlight, which can reliably report the dynamics of glycolysis in living cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Guldan Nazarbek, Aidana Kutzhanova, Lazzat Nurtay, Chenglin Mu, Bexultan Kazybay, Xugang Li, Cuiping Ma, Amr Amin, Yingqiu Xie
Summary: Researchers successfully predicted novel enzymes using a protein evolution analysis method, with experimental validation. Nanozymes are mainly classified as protein-based oxidoreductases, while peroxidase and lyase are the most conserved enzyme types in natural products. By simulating the evolution of natural protein enzymes, new natural nanozymes with lyase and peroxidase activities were discovered in Rhizoma polygonati and Goji herb extracts.
NANOSCALE ADVANCES
(2021)
Article
Microbiology
Kerstin Schultenkaemper, Desiree D. Guetle, Marina Gil Lopez, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch
Summary: The Gram-positive bacterium Bacillus methanolicus exhibits plasmid-dependent methylotrophy and possesses two fructose bisphosphate aldolases with distinct kinetic properties. Mutations in specific amino acid residues of these aldolases affect glycolytic and gluconeogenic activities. Gene expression regulation through CRISPR interference shows compensatory effects between the two aldolases, indicating a genetic mechanism for maintaining metabolic balance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Jose Ma. M. Angeles, Atcharaphan Wanlop, Minh-Anh Dang-Trinh, Masashi Kirinoki, Shin-ichiro Kawazu, Aya Yajima
Summary: Asian schistosomiasis caused by Schistosoma mekongi is mainly diagnosed by stool microscopy. However, serodiagnosis using ELISA with soluble egg antigen (SEA) has shown better sensitivity. This study aims to evaluate recombinant S. japonicum antigens for the detection of S. mekongi infection.
Article
Biochemistry & Molecular Biology
Seiya Shino, Ryo Nasuno, Hiroshi Takagi
Summary: This study reveals that nitrosative stress leads to protein S-glutathionylation in yeast cells, especially at the Cys112 site of Fba1 enzyme, inhibiting its activity. This S-glutathionylation promotes a metabolic shift from glycolysis to the pentose phosphate pathway (PPP), resulting in increased NADPH production and enhanced tolerance of yeast cells to nitrosative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Plant Sciences
Emma Sevilla, Pilar Andreu, Maria F. Fillat, M. Luisa Peleato, Juan A. Marin, Arancha Arbeloa
Summary: Excised root cultures can serve as an excellent experimental approach to study stress physiology and assess tolerance. The study demonstrates that protein changes in excised root cultures of Prunus cerasus under salt stress can be representative of changes in whole plants. Proteomic analysis revealed 16 relevant proteins expressed differentially in response to salt stress.
Article
Biology
Zarrin Basharat, Kanwal Khan, Khurshid Jalal, Sulaiman Mohammed Alnasser, Sania Majeed, Marium Zehra
Summary: Despite the significant role of fungal pathogens in invasive infections, they have been overlooked in computer aided therapeutic target mining and drug design. This study aims to identify drug targets and screen natural products with inhibition potential against Candida albicans. Several potential inhibitors were discovered, with ZINC13507461 showing promising bioavailability, warranting further laboratory testing.
Article
Plant Sciences
Kankan Peng, Yu Tian, Jing Cang, Jing Yu, Duojia Wang, Fuxia He, Huarui Jiao, Yige Tan
Summary: Overexpression of TaFBA-A10 in plants under freezing stress enhances FBA activity, increases expression of key enzymes in Calvin cycle and glycolysis, and ultimately improves freezing tolerance. TaFBA-A10 overexpressing lines exhibit lower electrical conductivity and MDA content, as well as higher reducing sugar content and ROS scavenging ability under freezing stress compared to wild type and fba mutants.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Oncology
Chuanzhuo Wang, He Xin, Guangxin Yan, Zhaoyu Liu
Summary: The aim of this study was to investigate the regulatory mechanism of lncRNA NONHSAG028908.3 on CRC. The results showed that silencing of NONHSAG028908.3 inhibited proliferation, migration, and invasion of CRC cells. Furthermore, NONHSAG028908.3 regulated ALDOA expression by sponging miR-34a-5p, promoting malignant activities in CRC.
Article
Biochemistry & Molecular Biology
Ying Zhao, Fangchan Jiao, Heng Tang, Houjuan Xu, Li Zhang, Hui Wu
Summary: This study identified and characterized 16 FBAs in Nicotiana tabacum, revealing two subfamilies located in chloroplast and cytoplasm. Most NtFBA genes showed positive responses to NaHCO3 stress, with NtFBA13/14 exhibiting a significant increase in expression. Moreover, NtFBA13/14 were highly up-regulated under five abiotic stress conditions, indicating their importance in photosynthesis and abiotic stress resistance.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Environmental Sciences
Jackson Nkoh Nkoh, Xian He, Hai-long Lu, Ke-wei Li, Ren-yong Shi, Jiu-yu Li, Ren-kou Xu
Summary: This study evaluates the effects of chitosan and F16BP on soil acidity and resistance to soil acidification. The results show that F16BP is more effective in improving soil acidity, while chitosan is better at enhancing soil pH buffering capacity, inhibiting soil acidification, and retarding the activation of phytotoxic aluminum.
JOURNAL OF SOILS AND SEDIMENTS
(2022)
Article
Infectious Diseases
Po-Ching Cheng, Ching-Nan Lin, Shih-Yi Peng, Tsung-Fu Kang, Kin-Mu Lee
PLOS NEGLECTED TROPICAL DISEASES
(2016)
Article
Immunology
Chien-Yu Lee, Po-Ching Cheng, Chen-Hsun Ho, Chia-Kwung Fan, David Chao
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2019)
Article
Immunology
Sabelo V. Dlamini, Chien-Wei Liao, Zandile H. Dlamini, Jameson S. Siphepho, Po-Ching Cheng, Ting-Wu Chuang, Chia-Kwung Fan
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2017)
Article
Multidisciplinary Sciences
Chen-Hsun Ho, Chia-Kwung Fan, Hong-Jeng Yu, Chia-Chang Wu, Kuan-Chou Chen, Shih-Ping Liu, Po-Ching Cheng
Article
Infectious Diseases
Jian Peng, Zhang-Ping He, Shuai Zhang, Zhao-Rong Lun, Zhong-Dao Wu, Chia-Kwung Fan, Christopher L. Brown, Po-Ching Cheng, Shih-Yi Peng, Ting-Bao Yang
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Article
Immunology
Ming-Hong Lin, Kin-Mu Lee, Che-Yuan Hsu, Shih-Yi Peng, Ching-Nan Lin, Chin-Chu Chen, Chia-Kwung Fan, Po-Ching Cheng
INTERNATIONAL IMMUNOPHARMACOLOGY
(2019)
Article
Microbiology
Chen-Hsun Ho, Yu-Chuan Lu, Chia-Kwung Fan, Hong-Jeng Yu, Hsin-Tien Liu, Chia-Chang Wu, Kuan-Chou Chen, Shih-Ping Liu, Po-Ching Cheng
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Article
Immunology
Chen-Hsun Ho, Chia-Hsiung Cheng, Tzu-Wen Huang, Shih-Yi Peng, Kin-Mu Lee, Po-Ching Cheng
Summary: Macrophages undergo phenotypic changes during Schistosoma japonicum infection, influencing the immune response towards a Th2- and B cell-skewed immunity.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Microbiology
Chen-Hsun Ho, Shih-Ping Liu, Chia-Kwung Fan, Kai-Yi Tzou, Chia-Chang Wu, Po-Ching Cheng
Summary: The study found that insulin pretreatment significantly reduced UPEC infection in bladder cells of diabetic individuals in a high-glucose environment, while also inhibiting the JAK/STAT transduction pathway, without affecting the expression of the insulin receptor.
Article
Biochemistry & Molecular Biology
Chen-Hsun Ho, Pin-Wen Liao, Chia-Kwung Fan, Shih-Ping Liu, Po-Ching Cheng
Summary: RNase 7 is a natural antibiotic that can inhibit urinary tract infection. This study demonstrates its antimicrobial and anti-inflammatory effects on bladder cells infected with Escherichia coli in a high-glucose environment, achieved by regulating the JAK/STAT signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Ho Yin Pekkle Lam, Meng-Yun Hung, Po -Ching Cheng, Shih-Yi Peng
Summary: The study investigates the therapeutic effect of combining wogonin, a liver-protective compound, with praziquantel (PZQ) in Schistosoma mansoni-infected mice. The results show that PZQ-wogonin treatment significantly improves liver pathology and reduces fibrosis, inflammation, and apoptosis in the liver. Additionally, wogonin exhibits anthelmintic properties and works synergistically with PZQ to reduce hepatic egg burden and disease progression.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Biochemistry & Molecular Biology
Pei-Chi Chen, Chen-Hsun Ho, Chia-Kwung Fan, Shih-Ping Liu, Po-Ching Cheng
Summary: JAK/STAT plays a key role in regulating UPEC infection in urothelial cells, and LCN2 pretreatment can dose-dependently suppress UPEC infections and downregulate TLR-4, STAT1, and STAT3 expression. LCN2 can alleviate UPEC infection by decreasing JAK/STAT pathway activation in a high-glucose environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Infectious Diseases
Ramdan Zulfa, Wei-Cheng Lo, Po-Ching Cheng, Martini Martini, Ting-Wu Chuang
Summary: This study conducted a systematic review and meta-analysis to investigate the insecticide resistance patterns in Asia from 2000 to 2021. The results revealed that insecticide resistance is widespread in both Ae. aegypti and Ae. albopictus in Asia, but the levels vary by country. Continuous monitoring of resistance markers and adjustment of control strategies will be crucial in preventing unexpected outbreaks.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Meeting Abstract
Infectious Diseases
Chien-Yu Lee, Po-Ching Cheng
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2017)
Article
Public, Environmental & Occupational Health
Chien-Wei Liao, Kuan-Chih Chiu, I-Chen Chiang, Po-Ching Cheng, Ting-Wu Chuang, Juo-Han Kuo, Yun-Hung Tu, Chia-Kwung Fan
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2017)
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)
