Journal
RESEARCH IN MICROBIOLOGY
Volume 164, Issue 10, Pages 979-986Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2013.08.007
Keywords
Shigellosis; Shigella flexneri; Siphoviridae; Bacteriophage; pSf-1
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) [2010-0016748]
- Ministry of Education, Science and Technology
- Korea Research Foundation Grant (gs2) [KRF-2008-331-E00385]
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Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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