Article
Biotechnology & Applied Microbiology
Stephen Olusanmi Akintayo, Behnoush Hosseini, Maliheh Vahidinasab, Marc Messmer, Jens Pfannstiel, Ute Bertsche, Philipp Hubel, Marius Henkel, Rudolf Hausmann, Ralf T. Voegele, Lars Lilge
Summary: This study evaluated the biocontrol ability of two new B. velezensis strains, ES1-02 and EFSO2-04, against fungal phytopathogens Diaporthe spp. and compared with reference strains QST713 and FZB42. The results showed that all strains were effective against the plant pathogens, with the new strains showing comparable antifungal activity to QST713 and slightly lower activity than FZB42.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Faisal Jan, Hamza Arshad, Mehreen Ahad, Asif Jamal, Donald L. Smith
Summary: The study aimed to explore the potential of Bacillus subtilis FJ3 for promoting plant growth and controlling fungal plant pathogens. The strain was evaluated for its growth-promoting traits and the ability to inhibit fungal pathogens through in vitro testing. The results showed that FJ3 had broad-spectrum antifungal activity and significantly reduced disease incidence in greenhouse experiments, demonstrating its potential for use in sustainable agriculture.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Danielle Santos-Lima, Cristina de Castro Spadari, Vinicius de Morais Barroso, Juliana C. S. Carvalho, Larissa Costa de Almeida, Felipe Santiago Chambergo Alcalde, Marcelo Jose Pena Ferreira, Miriam Sannomiya, Kelly Ishida
Summary: This study isolated and identified a lipopeptide-producing bacterium from a bacterial strain, which showed significant antifungal effects and potential for pharmaceutical development. The lipopeptides exhibited excellent performance in inhibiting fungal growth, disrupting fungal biofilms, and inducing morphological changes, making them promising antifungal agents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Joon Seong Park, Gwang Rok Ryu, Beom Ryong Kang
Summary: Bacillus subtilis KB21 was found to inhibit the growth and reproduction of plant pathogenic fungus Colletotrichum acutatum by producing antifungal lipopeptides, suggesting its potential as a biological control agent.
Article
Plant Sciences
Mahtab Omidvari, Gavin R. Flematti, Ming Pei You, Payman Abbaszadeh-Dahaji, Martin J. Barbetti
Summary: Phoma black stem and leaf spot disease can decrease forage quality and affect animal reproduction. Different cultivars of annual Medicago species show varying degrees of susceptibility to P. medicaginis, with different isolates of the pathogen having varying impacts. The levels of coumestrol and 4'-O-methylcoumestrol are correlated with disease severity across different cultivars.
Article
Biotechnology & Applied Microbiology
Zhen Li, Kleinberg X. Fernandez, John C. Vederas, Michael G. Ganzle
Summary: This study aims to investigate the interaction between Bacillus species and fungi in daqu models. It was found that B. velezensis FUA2155 displayed strong antifungal activity in the daqu model and was the only strain that produced multiple iturin A congeners in situ.
Article
Plant Sciences
Amanda Rosier, Maude Pomerleau, Pascale B. B. Beauregard, Deborah A. A. Samac, Harsh P. P. Bais
Summary: Plant growth-promoting rhizobacteria UD1022 showed antagonistic effects against several alfalfa pathogenic strains, making it a potential alternative to chemical crop treatments.
Article
Microbiology
Tingting Chen, Zhuzhu Zhang, Wenzhi Li, Jia Chen, Xuetang Chen, Bince Wang, Jiling Ma, Yunyun Dai, Haixia Ding, Weizhen Wang, Youhua Long
Summary: This study isolated a strain of Bacillus subtilis, named CTXW 7-6-2, from healthy kiwifruit tissue, which showed inhibitory effects on pathogenic fungi causing kiwifruit soft rot. The study identified the gene mechanisms and volatile compounds associated with the antifungal activity of CTXW 7-6-2, providing potential insights for the development of biocontrol strategies in the kiwifruit industry.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Organic
Diana Gimenez, Aoife Phelan, Cormac D. Murphy, Steven L. Cobb
Summary: The synthesis of fengycin A analogues with enhanced antifungal properties and chemical stability under acidic and basic conditions is reported. The analogues show that the fengycin core structure can be modified and simplified without loss of antifungal activity.
Article
Biotechnology & Applied Microbiology
Elaheh Taheri, Saeed Tarighi, Parissa Taheri
Summary: In this study, 10 endophytic bacteria were isolated from wheat seeds and one of them, identified as Bacillus subtilis CB2, showed biocontrol activity against Xanthomonas translucens and Fusarium graminearum. It was found that B. subtilis CB2 could produce various beneficial substances and reduce the infection and toxin production of the pathogens. Greenhouse trials demonstrated that application of B. subtilis CB2 increased the biomass and 1000-grain weight of the plants.
BIOLOGICAL CONTROL
(2023)
Article
Microbiology
B. N. Harish, S. N. Nagesha, B. N. Ramesh, S. Shyamalamma, M. S. Nagaraj, H. C. Girish, C. Pradeep, K. S. Shiva Kumar, K. S. Tharun Kumar, S. N. Pavan, V. Kavan Kumar
Summary: This study investigated the antifungal activities of lipopeptides from different B. subtilis strains against Alternaria alternata, and found that the T6 strain showed the highest antifungal activity with a suppression rate of 85.88%.
Article
Biotechnology & Applied Microbiology
Viviana Yanez-Mendizabal, Cesar E. Falconi, Kathleen Kanaley
Summary: The present study aimed to evaluate the use of a low-cost optimized medium (DSF40-MOL5) to improve the production of antifungal lipopeptides by Bacillus subtilis CtpxS2-1. Results showed that the population dynamics, pH, and oxygen consumption of CtpxS2-1 in DSF40-MOL5 were similar to that in the control medium. In DSF40-MOL5, there was a significant increase in the production of fengycin and iturin lipopeptides, as well as their antifungal activity. The expression of FenB and ItuA genes was higher in DSF40-MOL5 than in the control medium. Furthermore, treatment of infected seeds with CtpxS2-1 grown in DSF40-MOL5 effectively reduced anthracnose and improved seed and plant health.
BIOLOGICAL CONTROL
(2023)
Article
Microbiology
Konstantina Kourmentza, Xavier Gromada, Nicholas Michael, Charlotte Degraeve, Gaetan Vanier, Rozenn Ravallec, Francois Coutte, Kimon Andreas Karatzas, Paula Jauregi
Summary: Lipopeptide biosurfactants produced by Bacillus sp. exhibited high antifungal activity against food relevant fungi, while being not susceptible to Gram-positive and Gram-negative bacteria. Their antifungal activity was minimally affected by differences in isoform composition and purity, with low cytotoxicity. Combining nisin with lipopeptide biosurfactants can enhance the antimicrobial spectrum of action.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Gaurav Pal, Samiksha Saxena, Kanchan Kumar, Anand Verma, Deepak Kumar, Pooja Shukla, Ashutosh Pandey, Satish K. Verma
Summary: This study explores the potential of bacterial lipopeptides in eliciting defense responses in maize seedlings against Fusarium infection. The results showed that lipopeptide treatment enhanced protection against the fungus and improved the growth parameters measured. Gene expression analysis and metabolite analysis revealed the upregulation of defense-related genes and accumulation of antifungal polyphenols.
Article
Microbiology
Tanvir Ahmad, Fuguo Xing, Chengrong Nie, Changyu Cao, Ying Xiao, Xi Yu, Anam Moosa, Yang Liu
Summary: The Bacillus subtilis strain Y17B isolated from soil showed significant antifungal activity against Alternaria alternata, a pathogen causing postharvest fruit rot. The strain was found to produce antifungal lipopeptides, including surfactin, iturin, and fengycin, which effectively inhibited the growth of A. alternata. The study also demonstrated the alteration of A. alternata's morphology and the reduction of its growth in in vivo tests, highlighting the potential of B. subtilis Y17B as a biological control agent.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Rheumatology
Thierry Lequerre, Pascal Rottenberg, Celine Derambure, Pascal Cosette, Olivier Vittecoq
Article
Biochemistry & Molecular Biology
Siwar Soussi, Rym Essid, Julie Hardouin, Dorra Gharbi, Salem Elkahoui, Olfa Tabbene, Pascal Cosette, Thierry Jouenne, Ferid Limam
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2019)
Article
Plant Sciences
K. M. Hammi, R. Essid, O. Tabbene, S. Elkahoui, H. Majdoub, R. Ksouri
SOUTH AFRICAN JOURNAL OF BOTANY
(2020)
Article
Plant Sciences
O. Metoui Ben Mahmoud, R. Hidri, O. Talbi-Zribi, W. Taamalli, C. Abdelly, N. Djebali
SOUTH AFRICAN JOURNAL OF BOTANY
(2020)
Article
Horticulture
Safa Rguez, Imen Ben Slimene, Ghassen Abid, Majdi Hammemi, Asma Kefi, Salem Elkahoui, Riadh Ksouri, Ibtissem Hamrouni Sellami, Naceur Djebali
SCIENTIA HORTICULTURAE
(2020)
Article
Chemistry, Medicinal
Nesrine Gargouch, Fatma Elleuch, Ines Karkouch, Olfa Tabbene, Chantal Pichon, Christine Gardarin, Christophe Rihouey, Luc Picton, Slim Abdelkafi, Imen Fendri, Celine Laroche
Summary: Marine microalgae-derived exopolysaccharides (EPS) show promising biological activities, including antibacterial, anti-fungal, and anti-Candida albicans properties. EPS produced by Porphyridium marinum and its oligomers exhibit high efficiency in inhibiting bacterial growth, reducing biofilm formation, and affecting the viability of breast cancer cells.
Article
Agronomy
Manel Chaouachi, Takwa Marzouk, Selim Jallouli, Salem Elkahoui, Laurent Gentzbittel, Cecile Ben, Naceur Djebali
Summary: Botrytis cinerea is a major postharvest fungal pathogen in fresh produce, and the use of antagonistic microorganisms, particularly certain endophytic bacterial strains, shows promising potential in preventing decay in tomato fruit. These bacterial strains produce antifungal volatile organic compounds (VOCs) and some even have plant growth promoting effects. Specific VOCs such as 3-Methylbutan-1-ol are effective in protecting tomato fruit against B. cinerea infection, suggesting their potential use as biocontrol agents in reducing postharvest decay.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Agronomy
Mokhtar Elbekkay, Hammadi Hamza, Mohamed Hichem Neily, Naceur Djebali, Ali Ferchichi
Summary: The genetic diversity of local Tunisian watermelon cultivars and commercial varieties was studied using morphological traits and RAPD markers. Significant differences were found between commercial and local cultivars, with no correlation between genetic markers and geographical origin. The study provides valuable insights for establishing a core collection of Tunisian watermelon germplasm and for future breeding programs.
Article
Biochemistry & Molecular Biology
Siwar Soussi, Rym Essid, Ines Karkouch, Houda Saad, Sarra Bachkouel, Ezzedine Aouani, Ferid Limam, Olfa Tabbene
Summary: This study developed chitosan nanoparticles loaded with lipopeptides to improve their biological properties and reduce toxicity, demonstrating anti-adhesive and anti-infectious activities against Candida and Leishmania with low cytotoxicity.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ameni Ayed, Leila Kalai-Grami, Imen Ben Slimene, Manel Chaouachi, Houda Mankai, Ines Karkouch, Naceur Djebali, Salem Elkahoui, Olfa Tabbene, Ferid Limam
Summary: The study investigated the antifungal activity of volatile organic compounds from 10 Streptomyces isolates, with one strain S97 showing strong inhibitory effects, especially against Botrytis cinerea. The analysis of S97 VOCs revealed the presence of major terpenoids and phenolic compounds, which significantly inhibited the growth of various phytopathogenic fungi. In vivo tests on strawberries infected with Botrytis cinerea conidia demonstrated the potential of S97 VOCs to control grey mould symptoms.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Khaoula Mkadmini Hammi, Rym Essid, Nadine Khadraoui, Riadh Ksouri, Hatem Majdoub, Olfa Tabbene
Summary: This study investigated the antimicrobial and antioxidant activities of different fractions obtained from edible Tunisian Ziziphus Lotus leaves of Tozeur region. The dichloromethane fraction showed the highest activity against S. aureus and Methicillin-resistant S. aureus strains, as well as antileishmanial activity against L. major and L. infantum. Major flavonoids catechin, rutin, and luteolin 7-O-glucoside were found in the dichloromethane fraction, which may explain its antibacterial and antileishmanial activities. The butanolic fraction exhibited moderate antifungal activity, possibly due to the presence of chlorogenic acid. Additionally, both dichloromethane and butanolic fraction showed good DPPH scavenging activity and Ferric reducing power, indicating their potential as antioxidant and antimicrobial agents.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Nadine Khadraoui, Rym Essid, Selim Jallouli, Bilel Damergi, Iheb Ben Takfa, Ghassen Abid, Ines Jedidi, Asma Bachali, Ameni Ayed, Ferid Limam, Olfa Tabbene
Summary: This study evaluated the antibacterial and antibiofilm activities of Peganum harmala seed extract against multidrug-resistant Pseudomonas aeruginosa isolates, and identified the active compound and its molecular mechanism. The extract showed strong antibacterial and antibiofilm activities, indicating its potential as a candidate for the treatment of multidrug-resistant Pseudomonas aeruginosa biofilm infections.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ameni Ayed, Rym Essid, Slim Jallouli, Amal Ben Zaied, Sirine Ben Fdhila, Ferid Limam, Olfa Tabbene
Summary: The genus Streptomyces continues to be a major source of novel pharmaceutically relevant compounds with antimicrobial activity. A screening method was used to study ten strains of Streptomyces isolated from Tunisian soil and their activity against Candida albicans. One particular strain, S103, showed the highest inhibition rate against Candida albicans. Further analysis identified D-limonene as the most abundant volatile compound produced by this strain, which demonstrated strong antifungal activity.
EURO-MEDITERRANEAN JOURNAL FOR ENVIRONMENTAL INTEGRATION
(2022)
Article
Biotechnology & Applied Microbiology
Sarra Kefi, Rym Essid, Adele Papetti, Ghassen Abid, Lamjed Bouslama, Ezzedine Aouani, Olfa Tabbene, Ferid Limam
Summary: This study focused on the antibacterial and antileishmanial potential of Micromeria nervosa extracts. The active compound, ursolic acid, exhibited strong inhibitory effects on the promastigote and amastigote forms of L. infantum and L. major without cytotoxicity. Its molecular mode of action seems to involve the expression of specific genes.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Plant Sciences
Nouairi Issam, Djebali Naceur, Ghaya Nechi, Siwar Maatalah, Kais Zribi, Haythem Mhadhbi
Summary: This study synthesized zinc oxide nanoparticles using a green method and found that they have promising antifungal activity, making them a potential fungicide for the protection of faba beans against Botrytis cinerea.
ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION
(2021)