Article
Biotechnology & Applied Microbiology
Zhenbo Xu, Ziqi Liu, Thanapop Soteyome, Jingjing Hua, Liang Zhang, Lei Yuan, Yanrui Ye, Zhao Cai, Liang Yang, Ling Chen, Janette M. Harro, Birthe Veno Kjellerup, Junyan Liu, Yanyan Li
Summary: The study investigated the transcriptional regulation of the Cronobacter sakazakii pmrA mutant during biofilm formation, revealing important genes involved in different stages of biofilm formation.
Article
Food Science & Technology
Yan Ma, Yingying Zhang, Zhongguo Shan, Xin Wang, Xiaodong Xia
Summary: The study investigated the impact of PhoP/PhoQ two component system on biofilm formation in Cronobacter sakazakii BAA-894. Results showed that the system played crucial roles in regulating gene expression, cell viability, and extracellular polymeric substance content, suggesting it as a potential target for controlling biofilms.
Article
Food Science & Technology
Rajni Chauhan, Savita Kumari, Gunjan Goel, Wamik Azmi
Summary: This study determined the antibacterial and antibiofilm activity of six organic acids and various chemicals against C. sakazakii. The combination of malic acid with NaOCl showed the highest efficacy in inhibiting biofilm formation. The findings suggest that this combination has a synergistic effect and may be useful in eradicating C. sakazakii biofilms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Yifang Cao, Li Li, Yan Zhang, Fengsong Liu, Xinglong Xiao, Xiaofeng Li, Yigang Yu
Summary: The study shows that sdiA affects biofilm formation in C. sakazakii by regulating gene expression related to bacterial motility and biofilm characteristics. Biofilm formation increased under low osmotic stress conditions and decreased under high osmotic stress conditions.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Haoran Wang, Yulu Li, Zhuo Li, Run Ma, Xiangyang Bai, Xiangjun Zhan, Kunyao Luo, Ruiying Su, Xuejiao Li, Xiaodong Xia, Chao Shi
Summary: This study found that Litsea cubeba essential oil (LC-EO) has antimicrobial activity against Cronobacter sakazakii strains, and its mechanism involves increasing reactive oxygen species production, compromising cell membrane integrity, and causing cellular damage. Additionally, LC-EO effectively inhibited C. sakazakii biofilms and showed potential as a natural antimicrobial agent in the dairy industry.
Article
Food Science & Technology
Ping Li, Wenyue Zong, Zhengyang Zhang, Xuemeng Ji, Dongdong Zhu, Xinjun Du, Shuo Wang
Summary: In this study, the role of the flgK gene in the motility and desiccation resistance of C. sakazakii was investigated. The flgK mutant strain showed increased motility and invasion ability, while biofilm formation was not significantly affected. Interestingly, the absence of the flgK gene resulted in enhanced resistance to dehydration. Further analysis revealed changes in gene expression related to environmental stress resistance in the flgK mutant strain. This study provides valuable insights into the functions of the flgK gene and the molecular mechanism of desiccation resistance in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Microbiology
Jianxin Gao, Zhonghui Han, Ping Li, Hongyan Zhang, Xinjun Du, Shuo Wang
Summary: Research found that OmpF plays a positive regulatory role in the biofilm formation, adhesion, and invasion of C. sakazakii by reducing the amount of LPS, but acts as a negative regulator of antibiotic resistance.
Article
Food Science & Technology
Pianpian Yan, Xiuqin Chen, Ramachandran Chelliah, Kyoung hee Jo, Lingyue Shan, Hyunsoo Shin, Sunpyo Kim, Deog Hwan Oh
Summary: This study evaluated microbial contamination in the infant rice food industry, and assessed the effectiveness of slightly acidic electrolyzed water (SAEW) in preventing contamination. Pathogenic bacteria were found in various stages of the rice processing, and potential sources of cross-contamination were identified. SAEW was effective in reducing bacterial counts and eliminating biofilms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hui Li, Yi Zhao, Jingjing Zhang, Wen Li, Qixiu You, Xianxiang Zeng, Hengyi Xu
Summary: This study investigated the effects of silver nanoparticles (AgNP) on the environmental tolerance and biofilm formation of Cronobacter sakazakii. The results showed that AgNP significantly reduced the survival rate of C. sakazakii under environmental stress conditions, inhibited biofilm formation, and downregulated the expression of genes related to stress tolerance. AgNP also inhibited ATP production and biofilm formation in C. sakazakii cells.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Microbiology
Chuansong Cheng, Xiaotong Yan, Binxiong Liu, Tao Jiang, Ziwen Zhou, Fengting Guo, Qianwen Zhang, Changcheng Li, Ting Fang
Summary: The SdiA gene enhances drug resistance of Cronobacter sakazakii but decreases its motility, adhesion and biofilm formation ability without affecting its growth. Transcriptome analysis showed that knocking out SdiA upregulated the expression levels of certain genes and downregulated others, indicating that SdiA gene is related to the physiological metabolism of C. sakazakii. These findings are helpful for understanding the pathogenic mechanism and controlling bacterial infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Siyuan Gao, Jinhui Wu, Hong Wang, Shengying Hu, Li Meng
Summary: A rapid, sensitive, and quantitative detection method for Cronobacter sakazakii in powdered infant formula was developed using a SERS immunochromatographic test strip, with a high linear range and detection sensitivity.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Food Science & Technology
Xue Qin, Yitian Wang, Hongxuan Li, Xinyan Yang, Zhenghui Wang, Qing Chen, Chaoxin Man, Yujun Jiang
Summary: A visual detection strategy based on DNAzyme and asymmetry recombinase polymerase amplification (aRPA) was developed for monitoring Cronobacter sakazakii in powdered infant formula (PIF). The method showed low limits of detection and good stability in on-site simulation evaluation.
Article
Food Science & Technology
Lingli Luo, Lanhua Yi, Jiaxin Chen, Bianfang Liu, Xin Lu
Summary: Bacteriocin BM1157 inhibits Gram-negative bacteria by disrupting their metabolic processes, inhibiting biofilm formation, and ultimately causing cell death.
Article
Food Science & Technology
Yufei Fan, Ping Li, Dongdong Zhu, Chumin Zhao, Jingbo Jiao, Xuemeng Ji, Xinjun Du
Summary: This study aims to elucidate the precise roles of the ESA_00986 gene in C. sakazakii by generating gene knockout mutants and complementary strains. The results showed that the ESA_00986 gene plays a positive role in biofilm formation, cell adhesion and invasion, and virulence, demonstrating it as a novel virulence factor and advancing our understanding of the pathogenic mechanism of C. sakazakii.