Article
Food Science & Technology
Dianbo Zhao, Shaodan Wang, Yushan Hu, Xiao Liu, Jing Tao, Gianni Sagratini, Qisen Xiang
Summary: This study reveals the antibacterial mechanism of lauric arginate (LAE) against Escherichia coli. LAE damages the cytoplasmic and outer membranes of E. coli, causing a loss of membrane integrity. It also induces oxidative stress and increases reactive oxygen species levels. The combination of membrane damage and oxidative stress contributes to the antibacterial activity of LAE.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Do-Won Park, Jong-Hyun Park
Summary: In this study, a novel phage-derived depolymerase Dpo10 targeting the lipopolysaccharides of E. coli O157 was characterized, purified, and applied. Dpo10 showed potential as an anti-biofilm agent against E. coli O157:H7 strain by inhibiting biofilm formation and enhancing complement-mediated serum lysis activity against the bacteria.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Young-Min Bae, Hana Song, Sun-Young Lee
Summary: The study explored the effects of combining organic acids with various solutes on the survival of E. coli O157:H7 in laboratory conditions. It was found that combinations of acetic or malic acid with salt, glucose, glycine, or sucrose had different impacts on bacteria survival. Additionally, the presence of salt or sucrose in the solutions significantly altered the membrane fatty acid composition of the cells, potentially affecting bacterial tolerance to the organic acid treatments.
Article
Food Science & Technology
Haiying Cui, Xiaojing Yang, Changzhu Li, Yong Ye, Xiaochen Chen, Lin Lin
Summary: A strategy to simultaneously remove Escherichia coli O157 and its biofilms was successfully developed using composite nanofiber films. The films, synthesized by encapsulating E. coli O157 phages and D-phenylalanine into sodium alginate/polyethylene oxide nanofibers, showed excellent antibacterial activity against both free E. coli O157 and biofilms. The addition of D-phenylalanine significantly enhanced the inhibitory activity against E. coli O157 proliferation and biofilm formation during short-term storage of beef and fruits and vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xiaohua He, Heer Ding, Zenghui Gao, Xiaofeng Zhang, Rong Wu, Ke Li
Summary: The adaptability of Escherichia coli O157:H7 NCTC 12900 during noodle processing was investigated. The study found that bacterial surface morphology, count, and motility were impaired in the squeezing phase, while biofilm formation abilities increased across all processing phases. RT-qPCR analysis revealed that csrA, GcvB, McaS, and OxyS were the most relevant genes and sRNAs for biofilm formation and motility. Overexpression of these genes and sRNAs inhibited bacterial motility and biofilm formation to varying degrees during noodle processing.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Microbiology
Shuo Yang, Lan Ma, Xiaoqing Xu, Qing Peng, Huiying Zhong, Yuxin Gong, Linbo Shi, Mengxin He, Bo Shi, Yu Qiao
Summary: Rhamnolipid (RL) was found to inhibit biofilm formation of Escherichia coli O157:H7, but the mechanism is still unclear. Comparative physiological and transcriptomic analyses were conducted to elucidate the potential mechanism by which RL may inhibit biofilm formation in E. coli O157:H7. The results showed that RL treatment significantly reduced outer membrane hydrophobicity, affected cell membrane integrity and permeability by increasing the release of lipopolysaccharide (LPS), and led to differential expression of genes related to biosynthesis, outer membrane protein synthesis, flagellar assembly, and pathogenicity. Overall, RL treatment inhibits biofilm formation in E. coli O157:H7 by modifying outer membrane properties and gene expression levels.
Article
Biotechnology & Applied Microbiology
Wenbin Wang, Xinyue Zhou, Yunong Sang, Xiaxia Liang, Jianxin Liu, Saikun Pan, Luxin Wang
Summary: This study aimed to identify the target protein and epitope of the E. coli O157:H7-specific monoclonal antibody 2G12. The target protein was identified as OmpC, and the epitope was determined to consist of specific and conservative peptides LGIVING and TQTYNATRVGSLG. The stability and accessibility of the epitope on ECO157 cells grown in different environments were confirmed, highlighting its potential for use in various conditions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Yu-Bin Bai, Xiao-Rong Yang, Bing Li, Xu-Zheng Zhou, Wei-Wei Wang, Fu-Sheng Cheng, Ji-Yu Zhang
Summary: In this study, three AI-2 inhibitors (M414-3326, 3254-3286, and L413-0180) were obtained through virtual screening and their in vitro antibiofilm activities were validated. These inhibitors effectively inhibited biofilm formation of E. coli O157:H7 and are potential LuxS inhibitors that can be used to treat infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Raffaella Scotti, Bruno Casciaro, Annarita Stringaro, Fabrizio Morgia, Maria Luisa Mangoni, Roberta Gabbianelli
Summary: New strategies are needed to fight against multidrug-resistant bacteria caused by the overuse of antibiotics. Antibiofilm peptides derived from amphibian skin, such as Esc(1-21) and Esc(1-18), have shown potential to treat infections by inhibiting bacterial growth and biofilm formation. These peptides also induce the expression of genes associated with biofilm regulation and stress response in E. coli O157:H7.
Article
Chemistry, Analytical
Hyeonjeong Lee, Hyunsoo Han, Sangmin Jeon
Summary: In this study, a novel method for efficiently capturing waterborne bacteria was developed by creating an autonomous internal reflux of magnetic nanoparticle chains. By placing a glass tube containing positively charged PEI-coated MNCs at the center of a Halbach ring, a strong and uniform magnetic field was generated to capture target bacteria via electrostatic interaction. The capture efficiency of E. coli O157 was found to be over 90% at a flow rate of 15 mL/min, with detection limits of 2 and 3 cfu/mL in deionized water and river water, respectively.
ANALYTICAL CHEMISTRY
(2021)
Article
Veterinary Sciences
Shicheng Bi, Yiwen Qu, Jianjian Shao, Jianrong Zhang, Weihao Li, Li Zhang, Jingxuan Ni, Liting Cao
Summary: Ginsenoside Rg3 can protect broiler chicks from Escherichia coli lipopolysaccharide-induced stress by regulating inflammatory response and oxidative damage.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Yuanjie Suo, Weihong Yin, Wenshuai Wu, Wenjian Cao, Qiangyuan Zhu, Ying Mu
Summary: The thermosetting oil-based droplet array enables real-time monitoring and digital counting of bacterial growth. Single E. coli cells are encapsulated in solidified droplets to form microcolonies, and Resazurin is used to amplify the bacterial growth signals. This rapid and simple method does not require pre-enrichment and has been successfully applied for enumeration of E. coli in milk.
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Ecology
Elisa T. Granato, William P. J. Smith, Kevin R. Foster
Summary: Bacteria can collectively defend themselves against attacks from other strains using the T6SS, and the production of EPS can serve as a collective defense mechanism to protect both EPS-producing and non-producing cells. This has been confirmed by agent-based simulations and experiments.
Article
Biochemistry & Molecular Biology
Mauricio H. Pontes, Jinki Yeom, Eduardo A. Groisman
Article
Biochemistry & Molecular Biology
Jinki Yeom, Kyle J. Wayne, Eduardo A. Groisman
Article
Biochemistry & Molecular Biology
Jinki Yeom, Mauricio H. Pontes, Jeongjoon Choi, Eduardo A. Groisman
Article
Multidisciplinary Sciences
Jinki Yeom, Xiaohui Gao, Eduardo A. Groisman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Jinki Yeom, Woojun Park
Article
Microbiology
Jinki Yeom, Woojun Park
FEMS MICROBIOLOGY LETTERS
(2012)
Article
Biochemistry & Molecular Biology
Jinki Yeom, Yunho Lee, Woojun Park
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Microbiology
Jaejoon Jung, Jinki Yeom, Jiwon Han, Jisun Kim, Woojun Park
JOURNAL OF MICROBIOLOGY
(2012)
Article
Multidisciplinary Sciences
Yidan Cui, So Hyun Kim, Hyunseok Kim, Jinki Yeom, Kisung Ko, Woojun Park, Sungsu Park
Article
Multidisciplinary Sciences
Jinki Yeom, Ji-Hyun Shin, Ji-Young Yang, Jungmin Kim, Geum-Sook Hwang
Article
Microbiology
Jaejoon Jung, Jinki Yeom, Jisun Kim, Jiwon Han, Hyoun Soo Lim, Hyun Park, Seunghun Hyun, Woojun Park
RESEARCH IN MICROBIOLOGY
(2011)
Article
Cell Biology
Jinki Yeom, Eduardo A. Groisman
GENES & DEVELOPMENT
(2019)
Article
Multidisciplinary Sciences
Xiaohui Gao, Jinki Yeom, Eduardo A. Groisman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Jinki Yeom, Yi Shao, Eduardo A. Groisman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)