Review
Biotechnology & Applied Microbiology
Miho Takemura, Takehiko Sahara, Norihiko Misawa
Summary: Violaxanthin is biosynthesized from zeaxanthin through the xanthophyll cycle in photosynthetic eukaryotes to eliminate excessive light energy. However, commercial availability of violaxanthin and antheraxanthin is limited due to lack of efficient resource plants or organisms for their production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biology
Anamaria Babosan, David Skurnik, Anaelle Muggeo, Gerald B. Pier, Zeynep Baharoglu, Thomas Jove, Marie-Cecile Ploy, Sophie Griveau, Fethi Bedioui, Sebastien Vergnolle, Sophie Moussalih, Christophe de Champs, Didier Mazel, Thomas Guillard
Summary: PMQR genes promote high-level bacterial resistance to fluoroquinolone antibiotics, and we found that aminoglycosides can induce the SOS response, leading to elevated transcription of qnrD. The induction of SOS response is due to nitric oxide accumulation caused by sub-MIC of aminoglycosides.
Article
Multidisciplinary Sciences
Fei Shang, Hui Wang, Dan Zhang, Wenhui Wang, Jiangliu Yu, Ting Xue
Summary: In this study, the autoinducer-2 quorum sensing system was incorporated into the pET expression system, resulting in the construction of the autoinduction expression vector pXWZ1. The expression efficiency of the reporter genes was higher in cells transformed with pXWZ1 compared to traditional pET28a(+) vectors, suggesting that this new system will provide a more economical and convenient tool for recombinant protein expression.
Article
Biochemistry & Molecular Biology
Camila T. M. N. Porfirio, Pedro F. N. Souza, Marcio V. Ramos, Francisco A. P. Campos, Samuel F. Freitas, Joao P. B. Oliveira, Gilvan P. Furtado, Jose S. S. Barbosa, Thalia L. Frota, Celso S. Nagano, Rodolpho G. G. Silva, Ghulam Hussain, Cleverson D. T. Freitas
Summary: This study partially characterized three serine carboxypeptidases from Nepenthes mirabilis and further analyzed one peptidase in terms of its allergenic potential. The results showed that these enzymes have similar structures and mechanisms to other plant serine peptidases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Tomohiro Suzuki, Luna Nakamura, Satomi Inayoshi, Yuki Tezuka, Akiko Ono, Jae-Hoon Choi, Hideo Dohra, Tomohiro Sasanami, Hirofumi Hirai, Hirokazu Kawagishi
Summary: This study successfully increased the expression yield of Pleurocybella porrigens lectin using an Escherichia coli-based expression system, resulting in a 9-fold increase compared to previous studies. The recombinant lectin obtained maintained the same biochemical properties as the native lectin.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Radin Shafierul Radin Yahaya, Yahaya M. Normi, Lai Yee Phang, Siti Aqlima Ahmad, Janna Ong Abdullah, Suriana Sabri
Summary: This article discusses the importance of keratinase and various strategies to increase its production, including cloning and expression in native hosts as well as molecular strategies in heterologous hosts. These strategies aim to meet the large-scale demand for recombinant keratinases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Joana L. Rodrigues, Ligia R. Rodrigues
Summary: Furanocoumarins are plant secondary metabolites used for treating skin disorders, but extraction and purification are challenging, requiring new sustainable production schemes. While the heterologous production of simple coumarins has been demonstrated, the biosynthesis of more complex furanocoumarins remains largely unexplored.
NATURAL PRODUCT REPORTS
(2021)
Article
Fisheries
Wei-Chao Chen, Rui Ma, Tao Wang, Gao-Xue Wang
Summary: This study explored the recombinant expression of spring viraemia of carp virus glycoprotein using four different expression systems. The results showed that E. coli was the optimal expression system, but faced challenges such as the formation of insoluble aggregates. Yeast GS115 and insect Sf9 cells were also used and successfully produced the glycoprotein.
Review
Biology
Samia Falak, Muhammad Sajed, Naeem Rashid
Summary: This review focuses on various strategies to enhance the soluble yield of active recombinant proteins, including the choice of vector, host strain, culturing conditions, use of various fusion tags and chemical or biological chaperones.
Article
Biochemistry & Molecular Biology
S. Thiyagarajan, Priyal Khandelwal, N. Senthil, S. Vellaikumar, M. Arumugam, Abhishek Anil Dubey, S. Kathiresan
Summary: The study investigated the feasibility of using engineered E. coli to synthesize PUFAs, which successfully produced novel fatty acids of EPA and ARA as targeted products. This approach offers a potential alternative source for PUFA synthesis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Natalie S. Kolber, Ranan Fattal, Sinisa Bratulic, Gavriela D. Carver, Ahmed H. Badran
Summary: The study explores the potential of engineering heterologous ribosomes in living cells, discovering that optimized rRNA processing and supplementation with cognate r-proteins can enhance their function. It also notes reduced subunit exchange with host ribosomes for some heterologous ribosomes. This work provides a general framework for heterologous ribosome engineering and expands the use of heterologous parts in synthetic biology.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Muhammad Mustafa, Liaqat Ali, Waqar Islam, Ali Noman, Chengzeng Zhou, Linsong Shen, Taoting Zhu, Liu Can, Omaima Nasif, Kristina Gasparovic, Farooq Latif, Jiangtao Gao
Summary: This study focuses on the expression and purification of a thermostable glycoside hydrolase enzyme from the hyperthermophile Thermotoga maritima, which shows high stability in extreme environments such as acidic pH and high temperatures. The introduction of Ca2+ and Mn2+ further enhances the activity of the recombinant enzyme, suggesting its potential for industrial applications in hyperthermic conditions.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Plant Sciences
Rui Li, Ya Yang, Hanping Cao, Xiang Peng, Qin Yu, Linshen He, Ji Chen, Lien Xiang, Wanhong Liu
Summary: Heavy metal pollution in the soil poses a serious threat to both crop growth and human health. In this study, a new metallothionein encoding gene, NtMT2F, was cloned from Cd-hyperaccumulator tobacco and expressed in E. coli and A. thaliana to examine its biological function. The results showed that NtMT2F effectively enhanced resistance to heavy metal stress, particularly Cd, in both E. coli and transgenic A. thaliana. The findings suggest that NtMT2F could be a promising protein candidate for phytoremediation of heavy metal-contaminated environments.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yuki Nagao, Sangho Koh, Seiichi Taguchi, Tomohiro Shimada
Summary: Escherichia coli is used as a platform for producing valuable materials by introducing synthetic genes from other organisms. In this study, lactate-based polyester poly[LA-co-3HB] was produced in E. coli using five genes derived from different species. The expression level and timing of these genes were optimized to improve the production of P(LA-co-3HB) and manipulate the LA fraction. The results showed that the selection of an appropriate promoter for the expression of the phaC1STQK-AB operon can significantly enhance the production and LA fraction of P(LA-co-3HB). The findings suggest that using cell-growth phase-dependent promoters and selecting sugar-based carbon sources can be an effective strategy for intracellular production of polymeric materials.
MICROBIAL CELL FACTORIES
(2023)
Review
Biochemical Research Methods
Chuanteng Ma, Kaijin Zhang, Xianyan Zhang, Guowei Liu, Tianjiao Zhu, Qian Che, Dehai Li, Guojian Zhang
Summary: Terpenoids, the largest group of natural compounds, have made significant contributions to the pharmaceutical industry. Advances in synthetic biology and biological engineering have enabled the production of diverse pharmaceutical terpenoids in heterologous hosts, paving the way for developing advanced cell factories for terpenoid-based pharmaceuticals.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)