Article
Microbiology
Gayani Weerasooriya, Andrea R. McWhorter, Samiullah Khan, Kapil K. Chousalkar
Summary: Campylobacter is the most common bacterial cause of gastrointestinal disease, and contaminated poultry is often the source of infection. This study examines the survivability and virulence potential of sublethally injured Campylobacter in mice, following exposure to commonly used chemicals in poultry meat processing. The results show that sublethally injured Campylobacter jejuni has reduced virulence and colonization potential in mice.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Cecilia Johansson, Christian Kampmann, Anna Nilsson, Johan Dicksved, Lars Engstrand, Hilpi Rautelin
Summary: This study characterized Campylobacter jejuni fecal isolates from international travelers and analyzed the relationship between bacterial traits and clinical symptoms. It found that isolates from Bangladesh had higher pathogenic potential but led to milder symptoms compared to isolates from other regions with lower pathogenic potential but more severe symptoms. The study also examined the fecal microbiota composition prior to travel and found individual variation but no direct association with symptom development. Further research is needed to confirm the potential relationship between gut microbiota characteristics and the severity of Campylobacter infections.
Review
Immunology
Abdi Elmi, Fauzy Nasher, Nick Dorrell, Brendan Wren, Ozan Gundogdu
Summary: Research on Campylobacter jejuni has made significant progress, revealing emerging virulence factors that influence its pathogenicity. The spatial and temporal dynamics play a key role in promoting the bacterium to sense, adapt, and survive in multiple hosts. Future research should focus on obtaining a comprehensive understanding of C. jejuni virulence mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Se-Hun Kim, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Ayyappasamy Sudalaiyadum Perumal, Woo-Suk Bang, Momna Rubab, Eric Banan-Mwine Daliri, Kaliyan Barathikannan, Fazle Elahi, Eunji Park, Hyeon Yeong Jo, Su-Bin Hwang, Deog Hwan Oh
Summary: Campylobacter spp. are the leading cause of bacterial colon infections in humans globally, and their stress-adaptation mechanisms differ from other enteropathogenic bacteria, allowing them to survive in diverse environments and enhance their resistance mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Xuefeng Cao, Chris H. A. van de Lest, Liane Z. X. Huang, Jos P. M. van Putten, Marc M. S. M. Wosten
Summary: Research reveals that lysophospholipids of Campylobacter jejuni have toxicity to host cells, especially the short-chain lysoPEs (C:14) may be considered as a novel virulence factor.
Article
Multidisciplinary Sciences
Yuki Otsuka, Hideharu Hagiya, Misa Takahashi, Shinnosuke Fukushima, Ruri Maeda, Naruhiko Sunada, Haruto Yamada, Masayuki Kishida, Koji Fujita, Fumio Otsuka
Summary: In this retrospective, multicenter study, the clinical characteristics of Campylobacter bacteremia in western Japan were investigated. The study revealed that nearly half of the patients presented with fever as the main symptom, rather than gastrointestinal symptoms.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Leticia Silva Santos, Daise Aparecida Rossi, Raquelline Figueiredo Braz, Belchiolina Beatriz Fonseca, Micaela Guidotti-Takeuchi, Rosiane Nascimento Alves, Marcelo Emilio Beletti, Hebreia Oliveira Almeida-Souza, Larissa Prado Maia, Paula de Souza Santos, Jessica Brito de Souza, Roberta Torres de Melo
Summary: Despite being considered fragile and fastidious, Campylobacter jejuni is the most prevalent cause of foodborne bacterial gastroenteritis, especially through chicken meat transmission. Extreme stress can induce the acquisition of the viable but not culturable (VBNC) state in C. jejuni, which demonstrates its adaptability and virulence. The presence of transcripts, cell lysis, and specific metabolites in the VBNC form further emphasize the potential danger and difficulty in detection of this latent pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Anand B. Karki, Bhuwan Khatri, Mohamed K. Fakhr
Summary: RNA-seq was used to analyze the differentially expressed genes (DEGs) in C. coli HC2-48 and C. jejuni OD2-67 transcriptomes under different temperature conditions. In C. coli HC2-48, genes involved in flagellar assembly were significantly impacted by low temperatures (4°C), while genes related to the ribosome and ribonucleoprotein complex were modulated in C. jejuni OD2-67 at 4°C. Although more DEGs were observed in the chicken juice medium compared to Mueller-Hinton broth, there were no common genes expressed at all incubation times, indicating that the food matrix environment is not the sole determinant of differential expression in Campylobacter spp. at low temperatures.
Article
Microbiology
Hengchun Cao, Hanxiao Xu, Chunhui Ning, Li Xiang, Qiufang Ren, Tiantian Zhang, Yusen Zhang, Rui Gao
Summary: This study investigated the pangenome of Campylobacter jejuni, analyzing its virulence genes and potential core vaccine targets. Through experiments and analysis, five core vaccine targets with high antigenicity were identified, which play important roles in the virulence of C. jejuni in different hosts. The findings contribute to the discovery of new vaccine targets and provide a method to prevent multidrug-resistant C. jejuni.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Andrea Laconi, Roberta Tolosi, Ilenia Drigo, Luca Bano, Alessandra Piccirillo
Summary: This study assessed the ability of C. jejuni and C. coli strains isolated from diseased poultry to form biofilm. The results showed that C. jejuni strains were classified as no, weak, and moderate biofilm producers, while all C. coli strains were weak producers. Genes involved in chemotaxis, adhesion, and invasion were present in all C. jejuni strains. Further investigations are needed to better understand the genetic determinants involved in extra-intestinal Campylobacter biofilm formation.
VETERINARY MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Bodo Linz, Irshad Sharafutdinov, Nicole Tegtmeyer, Steffen Backert
Summary: Infection with Campylobacter jejuni causes campylobacteriosis, a common gastrointestinal infection. The disease is characterized by diarrhea lasting up to two weeks. C. jejuni possesses various peptidases and proteases that play critical roles in its cellular processes and pathogenesis. These enzymes are involved in protein quality control, protein transport, nutrient acquisition, biofilm formation, and virulence. Understanding the evolution of these proteases and their potential as targets for anti-bacterial therapies is also discussed.
Article
Biochemistry & Molecular Biology
Harald Nothaft, Xiaoming Bian, Asif Shajahan, William G. Miller, David T. Bolick, Richard L. Guerrant, Parastoo Azadi, Kenneth K. S. Ng, Christine M. Szymanski
Summary: The study identified C. jejuni isolates expressing a hexasaccharide lacking the glucose branch, caused by a missing aspartate in the DXDD motif of the glucosyltransferase PglI. Mouse vaccination with E. coli expressing the full-length heptasaccharide demonstrated reduction in C. jejuni colonization and weight loss. The study suggests the importance of considering glucose inclusion in N-glycan-based vaccines for preventing campylobacteriosis.
ACS CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mohamad Mortada, Douglas E. Cosby, Gabriel Akerele, Nour Ramadan, Jarred Oxford, Revathi Shanmugasundaram, Theros T. Ng, Ramesh K. Selvaraj
Summary: This study investigated the immune response of chickens to Campylobacter jejuni colonization, revealing that challenged birds showed persistent colonization in the ceca with subsequent dissemination to the spleen and liver. The increase in anti-Campylobacter antibodies in the serum and bile, suppression of T-lymphocytes, and changes in mRNA levels and cytokine production indicate a complex immune response. The balanced Th1 and Th2 immune responses against C. jejuni may play a role in bacterial colonization and the lack of pathology in challenged birds.
Article
Biology
Stephanie Peters, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan, Selina Jawahir, Jessica K. Calland, Evangelos Mourkas, Robin Patel, Terra Wiens, Marijke Decuir, David Boxrud, Kirk Smith, Craig T. Parker, Gianrico Farrugia, Qijing Zhang, Samuel K. Sheppard, Madhusudan Grover
Summary: Researchers used whole-genome sequencing and phenotypic analysis to identify potential genetic factors linked to irritable bowel syndrome caused by Campylobacter jejuni infection. They found that specific genetic variations in bacterial stress response, adhesion protein, and core biosynthetic pathway genes may contribute to the development of post-infection irritable bowel syndrome (PI-IBS). The study demonstrated that certain Campylobacter genotypes exhibit increased virulence in vitro, shedding light on the complex host-pathogen interactions underlying this condition.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Anja Klancnik, Ivana Gobin, Barbara Jersek, Sonja Smole Mozina, Darinka Vuckovic, Magda Tusek Znidaric, Maja Abram
Correction
Orthopedics
Tomislav Madarevic, Antea Buterin, Josko Jelicic, Luka Sirola, Darinka Vuckovic
INTERNATIONAL ORTHOPAEDICS
(2021)
Article
Medicine, General & Internal
Ivana Mikolasevic, Bozena Delija, Ana Mijic, Tajana Stevanovic, Nadija Skenderevic, Ivan Sosa, Irena Krznaric-Zrnic, Maja Abram, Zeljko Krznaric, Viktor Domislovic, Tajana Filipec Kanizaj, Delfa Radic-Kristo, Aleksandar Cubranic, Aron Grubesic, Radislav Nakov, Ivana Skrobonja, Davor Stimac, Goran Hauser
Summary: This study demonstrated a higher incidence of SIBO in NASH patients and those with SF, with significant predictors associated with SIBO including T2DM, fibrosis stage, and ballooning grade. Elastographic parameters did not show significant correlation with SIBO compared to histological characteristics of NAFLD.
INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
(2021)
Article
Infectious Diseases
Mirjana Stanic Benic, Dora Palcevski, Romina Milanic, Natasa Skocibusic, Maja Abram, Vera Vlahovic-Palcevski
Summary: The study aimed to decrease the incidence and resistance rates of ESBL Klebsiella pneumoniae by restricting the use of third-generation cephalosporins and fluoroquinolones. The results showed that the restriction of cephalosporin usage was associated with a decrease in ertapenem-resistant KP isolates, and an increase in fluoroquinolone usage predicted an increase in fluoroquinolone-resistant isolates. Overall, the study highlighted the complex association between antibiotic consumption and bacterial resistance, providing valuable insights for further research on intervention strategies.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Biochemical Research Methods
Marina Bubonja-Sonje, Lara Baticic, Maja Abram, Durdica Cekinovic Grbesa
Summary: Currently, there is insufficient evidence to determine whether SARS-CoV-2 antibodies provide protection from future infection and the duration of immunity. The kinetics of the immune response to SARS-CoV-2 infection and the role of serology in estimating individual protective immunity have yet to be established.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Chemistry, Physical
Andrej Pavlic, Gabrijela Begic, Marin Tota, Maja Abram, Stjepan Spalj, Ivana Gobin
Summary: The presence of nickel can affect the behavior and susceptibility of bacteria. Bacteria adapted to lower concentrations of nickel demonstrated better adhesion to nickel-titanium archwires, higher biofilm formation, and increased antimicrobial resistance compared to strains adapted to higher concentrations of nickel.
Article
Integrative & Complementary Medicine
Ivica Dimkic, Ivana Gobin, Gabrijela Begic, Davorka Repac Antic, Petar Ristivojevic, Karlo Jurica, Tanja Beric, Jelena Lozo, Maja Abram, Slavisa Stankovic
Summary: The antibacterial and antibiofilm effects of herbal leaf extracts were evaluated to control Enterococcus-induced urinary tract infections. The combination of bearberry tea and strawberry tree extracts showed a synergistic effect in inhibiting biofilm formation, which could be a potential alternative to combat antibiotic-resistant Enterococcus isolates.
JOURNAL OF HERBAL MEDICINE
(2021)
Article
Infectious Diseases
Mihaela Matovina, Maja Abram, Davorka Repac-Antic, Samira Knezevic, Marina Bubonja-Sonje
Summary: This study analyzed the distribution and antibiotic resistance patterns of ertapenem-resistant ESBL-producing K. pneumoniae strains recovered during an outbreak from 2012 to 2014 in a Croatian University hospital. The genetic relatedness of clinical isolates and underlying mechanisms that conferred the ertapenem-resistant phenotype were estimated. The study also highlighted the caution required regarding ertapenem-resistant, carbapenemase-negative porin-deficient mutants of K. pneumoniae as they could lead to local clonal outbreaks under selective pressure.
Article
Infectious Diseases
Silvestar Meznaric, Irena Brcic Karaconji, Goranka Crnkovic, Andrija Lesar, Tomislav Pavlesic, Darinka Vuckovic, Ivana Gobin
Summary: Honey is a natural food with nutritional and health value. Fir honeydew honey has antimicrobial and prebiotic properties, and its combination with L. plantarum has a synergistic effect against Salmonella enterica serotype Typhimurium.
Article
Biotechnology & Applied Microbiology
Andrej Pavlic, Ivana Gobin, Gabrijela Begic, Marin Tota, Maja Abram, Stjepan Spalj
Summary: This study investigated the interaction effects of ciprofloxacin and ampicillin with nickel on bacterial growth. The results showed that the interactions between the metal and antibiotics varied depending on the bacteria and the type of antibiotic.
FOLIA MICROBIOLOGICA
(2022)
Review
Microbiology
Tanja Grubic Kezele, Maja Abram, Marina Bubonja-Sonje
Summary: Most sinus infections are viral, but bacterial pathogens like Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis can also cause sinusitis during a viral respiratory infection. Alloiococcus otitidis is a commensal of the external auditory canal and a potential middle ear pathogen, rarely found in the nasopharynx of healthy individuals. This organism, difficult to culture, has been identified as a causative agent of sinusitis in a case study, presenting controversial findings based on recent literature.
Article
Public, Environmental & Occupational Health
Morana Tomljenovic, Danijela Lakoseljac, Lucija Knezevic, Marina Bubonja-Sonje, Maja Abram, Silvio Spicic, Maja Zdelar-Tuk, Sanja Duvnjak, Irena Reil, Orea Valjin, Melanija Kramaric, Tihana Miskic, Ivana Lohman Jankovic, Dobrica Roncevic
Summary: In March 2022, a non-occupational exposure outbreak of Q fever occurred in a semi-urban area in Cavle, Croatia. Veterinary and human epidemiological investigations identified airborne transmission as the most likely route of transmission. People living near the farms and in the nearest street had the highest risk of contracting Q fever.
ZOONOSES AND PUBLIC HEALTH
(2023)
Article
Pathology
Bojana Mohar Vitezic, Elena Stefancic, Davorka Repac Antic, Tanja Grubic Kezele, Maja Abram, Marina Bubonja-Sonje
Summary: This retrospective study followed up on borderline SARS-CoV-2 patients and found that 29.4% of them showed conversion from inconclusive to positive RT-PCR test results upon retesting. The results highlight the importance of retesting borderline patients with inconclusive results to reduce the potential risk of intrahospital transmission.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Article
Medicine, General & Internal
Ivana Mikolasevic, Goran Hauser, Maja Abram, Tajana Filipec Kanizaj, Marija Radic, Irena Krznaric Zrnic
CROATIAN MEDICAL JOURNAL
(2021)