Article
Chemistry, Organic
Xinyang Li, He-Ping Chen, Lin Zhou, Jing Fan, Takayoshi Awakawa, Takahiro Mori, Richiro Ushimaru, Ikuro Abe, Ji-Kai Liu
Summary: Cordycicadins A-D are four novel polyketides isolated from the liquid fermentation of Cordyceps cicadae JXCH1. Their structures were determined using spectroscopic analysis, single-crystal X-ray diffraction, and computational methods. Cordycicadins A and B showed antifeedant activity against silkworm larvae.
Article
Medicine, Research & Experimental
Xihua Yang, Lixia Chen, Lili Zhao, Yongming Yang, Jing Wang, Lei Yan, Gang Tai, Hong Zhang
Summary: The study aimed to investigate the therapeutic effect of Isaria felina on experimental autoimmune thyroiditis (EAT). The results showed that high-dose Isaria felina treatment significantly reduced circulating TSH, thyroid antibody, and cytokine levels in EAT mice. In addition, high-dose Isaria felina alleviated histopathological abnormalities and attenuated cell apoptosis in the thyroid tissue samples.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Peng Song, Wei Xu, Kuiming Wang, Yang Zhang, Fei Wang, Xiuling Zhou, Haiying Shi, Wei Feng
Summary: A predicted metalloproteinase gene, HypZn, was cloned from Aspergillus niger and expressed in Pichia pastoris, showing high activity and stability with good hydrolysis effect on soybean protein isolates.
Article
Biochemistry & Molecular Biology
Abdelsamed I. Elshamy, Tarik A. Mohamed, Tatsuro Yoneyama, Masaaki Noji, Sayaka Ban, Hiroshi Imagawa, Thomas Efferth, Mohamed-Elamir F. Hegazy, Akemi Umeyama
Summary: Cordyceps is a genus of fungi that includes edible varieties and those used in Chinese medicine. Chemical analysis of the entomopathogenic fungus Cordyceps bifusispora yielded four previously unknown coumarins, along with known metabolites. The compounds showed potential anti-tumor activity in cell viability assays, and the fungus was predicted to be a promising source of additional antitumor metabolites.
Article
Virology
Yuxiang Zhang, Najie Shi, Ping Wang, Qiuyan Zhu, Guogen Yang, Bo Huang
Summary: In this study, a novel double-stranded RNA mycovirus named Cordyceps chanhua alternavirus 1 (CcAV1) was discovered and characterized in the entomogenous fungus Cordyceps chanhua in China. The complete genome of CcAV1 consists of three segments encoding a putative RNA-dependent RNA polymerase and two hypothetical proteins. Phylogenetic analysis suggests that CcAV1 belongs to the proposed family Alternaviridae.
ARCHIVES OF VIROLOGY
(2022)
Article
Entomology
Di Wang, Pei-Xiang Xing, Hong-Liang Diao, Wen-Wen Zhou, Xian-Wei Li, Li-Jun Zhang, Rui-Yan Ma
Summary: This study investigated the pathogenicity of Cordyceps javanica IJ-tg19 to aphids and compared its characteristics with other entomopathogenic strains. Results showed that IJ-tg19 had higher virulence, faster conidial germination, and higher activity of cuticle-degrading enzymes compared to other strains. Microscopic observations revealed that IJ-tg19 could enter the hemocoel cavity of aphids, indicating its potential as an effective biological control agent for aphids.
Article
Biochemistry & Molecular Biology
Chuyu Tang, Xiuzhang Li, Tao Wang, Jie Wang, Mengjun Xiao, Min He, Xiyun Chang, Yuejun Fan, Yuling Li
Summary: This study evaluated the differences in composition and abundance of metabolites between Cordyceps sinensis and its substitutes through untargeted metabolomics. Differential metabolites and pathways were identified, with lipid metabolites being more abundant in Cordyceps sinensis, possibly contributing to its pharmacological functions.
Article
Microbiology
Ying Wang, Zhanshan Yang, Dapeng Bao, Bo Li, Xin Yin, Yingying Wu, Hongyu Chen, Guirong Tang, Nanyi Li, Gen Zou
Summary: Cordyceps militaris, a valuable bioresource, shows significant differences in growth and bioactive compounds synthesis under different cultivation methods. The adaptation to hypoxic conditions plays a crucial role in the growth and synthesis of bioactive compounds in C. militaris. Strategies to improve hypoxia adaption can have distinct effects on the production of bioactive compounds in C. militaris, offering insights for rational strain engineering and optimization strategies for cultivation and fermentation.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Xiuyun Wu, Tao Wu, Ailin Huang, Yuanyuan Shen, Xuanyu Zhang, Wenjun Song, Suying Wang, Haihua Ruan
Summary: The article systematically sorted out the latest research progress on the chemical characteristics, biosynthetic gene clusters and pathways of four typical bioactive ingredients in Cordyceps militaris. This summary will lay a foundation for obtaining low-cost and high-quality bioactive ingredients in large amounts using microbial cell factories in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Eliot T. Smith, Michael Kruppa, David A. Johnson, Jessica Van Haeften, Xingchen Chen, Darren Leahy, Jonathan Peake, Jonathan M. Harris
Summary: Recombinant human neutrophil elastase (rHNE) was successfully expressed, purified, and confirmed to have the same activity and substrate affinity as the native enzyme. Major modifications were made to prevent hyper-glycosylation and optimize the translation in Pichia pastoris. This study provides a valuable foundation for further research on this important human neutrophil enzyme.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Biochemistry & Molecular Biology
Chaoqun Tong, Tian Li, Shisui Luo, Ruoni Chen, Shijiang Chen, Junhong Wei, Yuling Qing, Shaorong Qin, Guoqing Pan, Chunfeng Li, Zeyang Zhou
Summary: A PCR primer pair was designed to detect Cordyceps farinosa and Samsoniella hepiali in soil samples and dead Thitarodes armoricanus larvae samples. This rapid detection method provides important information for controlling C. farinosa during Ophiocordyceps sinensis cultivation.
CANADIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sitao Wen, Guogan Wu, Huawei Wu
Summary: The newly discovered xylanase ArXyn10 from Anaeromyces robustus was cloned and expressed in Pichia pastoris, showing optimal activity at pH 5.5 and 40°C. ArXyn10 exhibited good stability and was able to increase reducing sugar content in bread making, improving the quality of bread.
Article
Microbiology
Peipei Wu, Qilian Qin, Jihong Zhang, Huan Zhang, Xuan Li, Hongtuo Wang, Qian Meng
Summary: The temporal and spatial regulation of entry of O. sinensis into ghost moths was investigated, revealing that the invasion process does not require specific differentiated structures, providing a new perspective on the invasion process of entomopathogenic fungi.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenxiu Wang, Fengmao Chen
Summary: The study obtained the active recombinant CJPRB protein through the Pichia pastoris expression system. It was found that injection of CJPRB protein could induce a more rapid, widespread and intense immune response in H. cunea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Hongbo Jiang, Jinghui Liu, Jie Bao, Yuenan Xing, Chengcheng Feng, Qingbiao Hu, Xiaodong Li, Qijun Chen
Summary: This study establishes two recombinase polymerase amplification (RPA) detection methods to efficiently diagnose and prevent microsporidia infection in Chinese grass shrimp. These methods show high specificity, sensitivity, and short detection time, which can simplify the detection process and improve sample analysis speed.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Plant Sciences
Ting Yang, Yi Xin, Tongyao Liu, Zhengfeng Li, Xingzhong Liu, Yunpeng Wu, Mingfeng Wang, Meichun Xiang
Summary: This study identified a rhizosphere bacterium, strain NC1, which showed strong inhibition against root-knot nematodes. Gas chromatography-mass spectrometry analysis revealed that the strain produced volatile compounds, DMDS and benzaldehyde, that exhibited nematicidal activity. A greenhouse trial demonstrated that the strain effectively controlled root-knot nematodes.
Article
Biotechnology & Applied Microbiology
Shu Zhang, Shuang Wang, Zemin Fang, B. Franz Lang, Yong-Jie Zhang
Summary: The study presents the complete mitogenome assembly of Gongronella sp. w5 and reveals substantial differences in mitogenome size, intron number, gene order, and orientation from other Mucoromycota species. This unique mitogenome information enhances our understanding of fungal evolution in Mucoromycota.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Mycology
Wenjia Li, Jing Li, Xuefeng Hu, Linghua Xu, Xiaoyu Liu, Zhengming Qian, Liling Jin, Yimin Zhang, Jiangchun Wei, Xingzhong Liu
Summary: Chinese cordyceps extract (CCE) has been found to exhibit anti-tumor effects through the suppression of tumor angiogenesis, making it a potential treatment for angiogenesis-related cancers.
MYCOLOGICAL PROGRESS
(2022)
Article
Microbiology
Meichun Xiang, Jun Chu, Wenjiao Cai, Haikun Ma, Weijing Zhu, Xiaoling Zhang, Jinwei Ren, Lizheng Xiao, Dongbo Liu, Xingzhong Liu
Summary: This study tracked microbial succession and interactions during the development of the golden flower fungus in Fu Brick tea using culture-dependent methods and high-throughput sequencing. The study found that the presence of the golden flower fungus was associated with microbes such as Klebsiella, Lactobacillus, and Bacillus, highlighting the importance of these co-occurring microbes in the manufacturing process of Fu Brick tea.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Zongkui Wang, Huiwen Xiao, Jiali Dong, Yuan Li, Bin Wang, Zhiyuan Chen, Xiaozhou Zeng, Jia Liu, Yanxi Dong, Li Ma, Jun Xu, Lu Cheng, Changqing Li, Xingzhong Liu, Ming Cui
Summary: Intravenous immunoglobulin (IVIg) has been investigated as a potential treatment for radiation injuries. The results showed that IVIg can protect against radiation-induced hematopoietic and gastrointestinal toxicity in female mice but not in males. In addition, IVIg can modulate gut microbiota configurations in both females and males. Overall, IVIg shows potential as a sex-specific therapeutic avenue to improve the prognosis of cancer patients undergoing radiotherapy.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Microbiology
Xiaozhou Zhao, Yani Fan, Meichun Xiang, Seogchan Kang, Shunxian Wang, Xingzhong Liu
Summary: The Ca2+/calmodulin-dependent signaling pathway plays critical roles in regulating growth, conidiation, and stress response in nematode-trapping fungi. The deletion of the Crz1 gene (DdaCrz1) in Drechslerella dactyloides significantly reduced hyphal growth and trap formation, and altered sensitivity to various ions and stress conditions. This study highlights the importance of the Ca2+/calmodulin-dependent signaling pathway in trapping nematodes.
Article
Microbiology
Yue Chen, Jia Liu, Yani Fan, Meichun Xiang, Seogchan Kang, Dongsheng Wei, Xingzhong Liu
Summary: DdVam7 protein plays a crucial role in D. dactyloides, regulating fungal growth, conidiation, and predatory process by mediating vacuole assembly, providing a basis for studying mechanisms of NTF and ring cell rapid inflation.
MICROBIOLOGY SPECTRUM
(2022)
Article
Plant Sciences
Peinan Fu, Yang Zhang, Chenyin Wang, Shuyi Si, Xingzhong Liu, Yongsheng Che
Summary: Four new 9,11-secosteroid-derived gamma-lactones, called Altersteroids A-D (1-4), were isolated from a culture of the ascomycete fungus Alternaria sp. Their structures were determined primarily by NMR experiments. The absolute configuration of compound 1 was established through X-ray crystallographic analysis, while the configurations of compounds 2-4 were assigned using quantum-chemical calculations. These compounds feature a gamma-lactone moiety fused to the steroid D ring at C13/C-14. Compound 3 exhibited moderate cytotoxicity against four tumor cell lines and induced apoptosis in A549 cells. Notably, compound 3 showed equipotent activity against cisplatin-sensitive and cisplatin-resistant MB49 cells, with an IC50 value of 12.7 μM.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Plant Sciences
Yang Zhang, Peinan Fu, Yiqing Zhang, Yang Xu, Chunyan Zhang, Xingzhong Liu, Yongsheng Che
Summary: In this study, two new xanthone derivatives, Cladoxanthones A and B, as well as a known compound, mangrovamide J, were isolated from the culture of Cladosporium sp. The structures and absolute configurations were determined using NMR and X-ray crystallography. These compounds exhibited weak cytotoxic effects.
JOURNAL OF NATURAL PRODUCTS
(2022)
Correction
Biology
Wenjia Li, Jinming Xia, Quanping Li, Zongyao Zhang, Weiwei Zhang, Caihong Dong, Jiangchun Wei, Xingzhong Liu
Summary: This paper contains errors in the authors' affiliations. Wenjia Li is a Ph.D. candidate at the University of Chinese Academy of Sciences, while Jiangchun Wei and Xingzhong Liu are professors at the same university, which was missing in the original version. The affiliations have been corrected.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Review
Mycology
Shunxian Wang, Xingzhong Liu
Summary: This paper summarizes the development and progress of gene manipulation tools in nematode-trapping fungi (NTF), including transformation methods and strategies, random gene mutagenesis methods, and target gene mutagenesis methods. It provides a clear reference and instruction for researchers working on NTF as well as other groups of fungi.
MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY
(2023)
Article
Mycology
Jingzu Sun, Shuang Yu, Yongzhong Lu, Hongwei Liu, Xingzhong Liu
Summary: During a field survey, two causal agents of mushroom diseases - Diploospora longispora and Paecilomyces penicillatus - were identified, causing up to 80% of yield losses. Phylogenetic analysis revealed that these fungi belong to a distinct clade in Hypocreales. Two new genera, Pseudodiploospora and Zelopaecilomyces, were introduced based on their morphological characteristics and phylogenetic relationships. Five new combinations were proposed.
MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY
(2023)
Article
Genetics & Heredity
Xiaozhou Zhao, Yani Fan, Weiwei Zhang, Meichun Xiang, Seogchan Kang, Shunxian Wang, Xingzhong Liu
Summary: Calcium ion (Ca2+) serves as a universal second messenger in regulating various processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) is responsible for acquiring Ca2+ from the extracellular environment under high extracellular Ca2+ concentration. Nematode-trapping fungi (NTF) encode two related proteins for LACS, and this study focused on characterizing the role of DhFIG_2, an ortholog of AoFIG_2, in growth and development of Dactylellina haptotyla. Knocking down the expression of DhFIG_2 via RNA interference (RNAi) significantly impacted conidiation, trap formation, vegetative growth, and stress responses, indicating the importance of this LACS component in NTF.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Chemistry, Medicinal
Wenjia Li, Qinggui Lei, Wenqing Li, Guoying Tan, Xingzhong Liu, Zhengming Qian
Summary: A high-performance liquid chromatography (HPLC) method using one reference compound was developed for the determination of 4 nucleosides in Chinese cordyceps. The method involved ultrasonic extraction of the cordyceps sample with a 0.5% phosphoric acid solution, followed by separation on a CORTECS T3 column using acetonitrile and water as the mobile phase. The established HPLC method showed good accuracy, precision, reproducibility, and stability, making it suitable for the quantitative analysis of nucleosides in Chinese cordyceps. The method is a simple, cost-effective, and time-saving alternative for quality control of Chinese cordyceps.
NATURAL PRODUCT COMMUNICATIONS
(2023)
Correction
Chemistry, Analytical
Wenjia Li, Zhengming Qian, Chunhong Li, Dean Guo, Jiangchun Wei, Xingzhong Liu
ANALYTICAL LETTERS
(2022)
