Article
Endocrinology & Metabolism
Xi Zhang, Li Li, Wenhong Zhang, Yang Luo, Yuling Mao, Hongzi Du, Lei Li
Summary: This study compares the outcomes of different types of sperm retrieved using microdissection testicular sperm extraction (micro-TESE) in patients with non-obstructive azoospermia (NOA). The results indicate that artificial oocyte activation (AOA) can improve fertilization rate but does not affect embryo quality and live birth outcomes. AOA is recommended only when immotile sperm are injected.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Jing Ling Ruan, Shan Shan Liang, Jia Ping Pan, Zhi Qin Chen, Xiao Ming Teng
Summary: This retrospective study found that artificial oocyte activation (AOA) with calcium ionophore ionomycin can significantly improve the reproductive outcomes of patients with previous fertilization failure and poor embryo development after intracytoplasmic sperm injection (ICSI). AOA can increase fertilization rate, embryo quality, implantation rate, clinical pregnancy rate, and live birth rate, while decreasing miscarriage rate. The study suggests that AOA is an effective and safe treatment option for some infertility patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Sophie Popkiss, Fabrizzio Horta, Beverley Vollenhoven, Mark P. Green, Deirdre Zander-Fox
Summary: This study aimed to investigate the effect of calcium chloride dihydrate supplementation on different fertilization rate groups during the ICSI process. The results showed that adding calcium significantly improved fertilization rate and clinical pregnancy rate in patients with low fertilization rates (< 50%), but had minimal impact on patients with normal fertilization rates.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Review
Physiology
Yinghua Shan, Huishan Zhao, Dongmei Zhao, Jianhua Wang, Yuanqing Cui, Hongchu Bao
Summary: This meta-analysis demonstrates the efficacy and safety of using calcium ionophore for assisted oocyte activation. Calcium ionophore significantly increases clinical pregnancy and live birth rates without increasing the risk of adverse pregnancy outcomes.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Endocrinology & Metabolism
Xiao Fu, Yiting Zhang, Shuli Gao, Shuzhe Gao, Meng Zhang, Shanshan Gao, Jinlong Ma, Zi-Jiang Chen
Summary: Different indications for oocyte vitrification do not affect clinical pregnancy rates. The clinical pregnancy rate of vitrified oocytes for different indications is correlated with age and number of thawed oocytes. For women with an abundant number of retrieved oocytes, cryopreserving a small number of oocytes is a valuable option that may benefit them in the future. Additional studies with large-scale, variable-controlled methodologies and extended follow-up are needed to further validate and clarify these findings.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Xiaokun Hu, Yuliang Liu, Xiubing Zhang, Pingyin Lee, Yangxing Wen, Chenhui Ding, Canquan Zhou, Yanwen Xu
Summary: This study aimed to investigate the impact of oocyte degeneration after ICSI on cumulative live birth rate in young women; The results showed that oocyte degeneration is not an indicator for predicting the cumulative live birth rate.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
Tzung-En Tsai, Pei-Hsuan Lin, Pei-Fen Lian, Chia-Jung Li, Salvatore Giovanni Vitale, Mislav Mikus, Wan-Ping Su, Hsiao-Wen Tsai, Kuan-Hao Tsui, Li-Te Lin
Summary: AOA may improve embryo quality in older patients with diminished ovarian reserve undergoing IVF-ICSI cycles, especially in women aged >= 40 years old.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Obstetrics & Gynecology
Amanda Souza Setti, Gabriela Halpern, Daniela Paes de Almeida Ferreira Braga, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: Maternal lifestyle factors, such as smoking, alcohol consumption, refined sugar, and artificial sweeteners, have a significant impact on the incidence of oocyte dimorphisms and outcomes of intracytoplasmic sperm injection (ICSI) cycles. These habits are positively associated with oocyte dimorphisms and negatively associated with ovarian stimulation response, embryo development, clinical pregnancy rates, and live birth rates. The associations are dose-dependent.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Agriculture, Dairy & Animal Science
Jana Rychtarova, Alena Langerova, Helena Fulka, Pasqualino Loi, Michal Benc, Josef Fulka
Summary: The long-term storage of biological material is crucial for various fields, and a simple approach for evaluating sperm DNA damage level has been proposed to address the issue of limited frozen biological material quantities.
Article
Endocrinology & Metabolism
Nan Peng, Shuiying Ma, Cheng Li, Hui Liu, Haibin Zhao, Lian-Jie Li, Qing Li, Mei Li
Summary: This study aimed to explore the clinical application value of half-ICSI treatment for infertility in assisted reproductive technology. The results showed that ICSI treatment improved laboratory results compared with IVF treatment, but there were no significant differences in clinical pregnancy and live birth rates. Therefore, the use of ICSI as an extra intervention should be judicious.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Nowak Agnieszka, Kochan Joanna, Witarski Wojciech, Okolski Adam
Summary: This study aimed to assess the viability and developmental competence of equine oocytes after in vitro maturation and vitrification using the Rapid-I method. The results showed that in vitro matured oocytes vitrified in EquiproVitKit media had a high survival rate.
Article
Obstetrics & Gynecology
Xiaolei Chen, Haibin Zhao, Jiale Lv, Yi Dong, Maoning Zhao, Xinlei Sui, Ran Cui, Boyang Liu, Keliang Wu
Summary: This study investigates the efficacy of calcium (Ca2+) ionophore treatment in improving embryo development and pregnancy outcomes in patients with poor embryo development in previous intracytoplasmic sperm injection (ICSI) cycles. The results show that the use of calcium (Ca2+) ionophore significantly improves preimplantation development and pregnancy rates.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Article
Genetics & Heredity
Jose Buratini, Clarissa Moutier, Maria Cristina Guglielmo, Diana Turchi, Robert Webb, Elena De Ponti, Mario Mignini Renzini, Mariabeatrice Dal Canto
Summary: This study aimed to assess the effects of oocyte central granularity and its underlying endocrine environment on the developmental competence of dysmorphic and morphologically normal oocytes. A retrospective cohort study was conducted with 1,082 patients undergoing autologous ICSI cycles. Results showed that CG oocytes had severely compromised developmental competence and a lower live birth rate compared to NCG and N oocytes. Maternal age should be considered when deciding whether to use CG oocytes in ICSI cycles.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Obstetrics & Gynecology
Bei Sun, John Yeh
Summary: This paper reviews the effects of calcium oscillatory patterns in oocytes and early embryo development, suggesting that abnormal calcium oscillations may be the primary mechanism for total fertilization failure (TFF). Studies also found strong associations between abnormal calcium oscillatory patterns and suboptimal developmental outcomes. Empirical use of artificial oocyte activation (AOA) methods has shown initial success in helping TFF patients.
REPRODUCTIVE SCIENCES
(2021)
Article
Obstetrics & Gynecology
Lei Jia, Panyu Chen, Wenlong Su, Shujing He, Yingchun Guo, Lei Zheng, Cong Fang, Xiaoyan Liang
Summary: This retrospective cohort study compared the fertilization rates in ICSI cycles treated with artificial oocyte activation (AOA) using 10 mu mol/l ionomycin or commercial A23187. The results showed that the fertilization rates were significantly higher in the ionomycin group compared to the A23187 group for all subgroups. Therefore, AOA with 10 mu mol/l ionomycin may be more effective in improving oocyte activation, especially in cases of sperm-related defects.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Biochemistry & Molecular Biology
Shahnaz Babaei-Abraki, Fereshteh Karamali, Mohammad Hossein Nasr-Esfahani
Summary: This study demonstrates that iron accumulation and lipid peroxidation are responsible for cell death in dissociated human embryonic stem cells (hESCs). P53 mediates iron-induced cell death, while the nuclear factor erythroid 2-related factor 2 (Nrf2) inhibits cell death by regulating multiple genes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Sareh Soroushzadeh, Fereshteh Karamali, Elahe Masaeli, Atefeh Atefi, Mohammad Hossein Nasr Esfahani
Summary: The study introduces a unique scaffold-free method for RPE cell sheet engineering, which reconstructs the basal membrane of cells through cell sheet engineering and provides a new approach for retina regeneration and treatment.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Cell Biology
Milad Abdollahi, Sayyed Mohammad Marandi, Kamran Ghaedi, Zahra Safaeinejad, Fatemeh Kazeminasab, Samaneh Shirkhani, Mohammad Hossein Sanei, Parsa Rezvanian, Mohammad Hossein Nasr-Esfahani
Summary: In this study, it was found that the combination of aerobic training with green coffee or chlorogenic acid supplementation can improve complications in prediabetic mice and have an effect on genes related to liver and glucose metabolism regulation.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Andrology
Marziyeh Tavalaee, Mohsen Rahmani, Joel R. Drevet, Mohammad Hossein Nasr-Esfahani
Summary: This review investigates the potential role of inflammasome pathway (IP) activation in male infertility. The results suggest that inflammasome activation and its consequences, including cytokine storms, apoptosis, and pyroptosis, may be associated with reduced sperm count and structural and functional sperm defects. This indicates that anti-inflammatory therapeutic strategies may be considered for restoring male fertility in future research.
BASIC AND CLINICAL ANDROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hanieh Hadady, Fereshteh Karamali, Fatemeh Ejeian, Sareh Soroushzadeh, Mohammad Hossein Nasr-Esfahani
Summary: This study evaluated two methods (micro-electromechanical systems and ultrafiltration) for isolating extracellular vesicles (EVs) from stem cells from apical papilla (SCAP). It found that different methods enriched different subsets of EVs, which significantly impacted their biological and functional effects. Further experiments confirmed the neuroprotection and anti-inflammatory capacity of SCAP-EVs in vitro, as well as their potential therapeutic effects in a retinal degeneration model.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Parisa Nadri, Saeid Ansari-Mahyari, Farnoosh Jafarpour, Amir Hossein Mahdavi, Nima Tanhaei Vash, Liana Lachinani, Kianoush Dormiani, Mohammad Hossein Nasr-Esfahani
Summary: The addition of melatonin to the culture medium can accelerate blastocyst formation and improve the quality of ovine SCNT embryos by reducing oxidative stress, apoptosis, and promoting telomere elongation. These findings offer new possibilities for the development of ovine SCNT embryos.
Article
Andrology
Fatemeh Karevanpour, Marziyeh Tavalaee, Fatemeh Kazeminasab, Milad Abdollahi, Samaneh Shirkhani, Mohsen Rahmani, Kamran Ghaedi, Sayed Mohammad Marandi, Mohammad Hossein Nasr-Esfahani
Summary: This study investigated the effects of green coffee and chlorogenic acid as active ingredients, as well as exercise, on spermatogenesis and sperm function in pre-diabetic mice. The results showed that pre-diabetic status had adverse effects on spermatogenesis and sperm quality. Exercise improved sperm motility, while green coffee supplementation reduced DNA damage and chlorogenic acid supplementation significantly improved motility. These findings suggest potential approaches for improving sperm quality in individuals with diabetes.
Article
Biology
M. Zanjirband, M. Baharlooie, Z. Safaeinejad, M. H. Nasr-Esfahani
Summary: This study investigated the novel therapeutics targets in PCOS by analyzing the transcriptome of granulosa cells using a drug repositioning approach. The results could provide valuable insights for the management of PCOS.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani
Summary: The role of the WNT/beta-catenin signaling pathway in preimplantation goat development was investigated. The expression of beta-CATENIN was observed in IVF and SCNT embryos, and inhibition of beta-catenin resulted in increased blastocyst formation rate. Different localization patterns of beta-catenin were observed in IVF and SCNT blastocysts. Inhibition of WNT signaling during the compact morula to blastocyst transition improved preimplantation embryonic development.
Article
Obstetrics & Gynecology
Aram Minas, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Hashemi-Asl, Marziyeh Tavalaee
Summary: Varicocele (VCL) induces severe oxidative stress in testicular tissue, leading to infertility in 35% of males. This study compares the impact of varicose vessels on oxidative DNA damage and antioxidant reactions in the seminiferous tubules. The results show that varicocele causes significant DNA damage, reduces antioxidant reactions, and negatively affects the cell cycle progression.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maryam Mirani, Soghra Bahmanpour, Fatemeh Masjedi, Zahra Derakhshan, Mahintaj Dara, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Bagher Tabei
Summary: This study reveals the negative effects of AGEs-RAGE axis on steroidogenesis in luteinized GCs, which can be mitigated by PM treatment. Understanding the interaction between AGEs and PM in ovarian physiology and pathology may lead to more targeted therapy for ovarian dysfunction.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Ali Moghimi-Khorasgani, Farshad Homayouni Moghadam, Mohammad Hossein Nasr-Esfahani
Summary: Microglial cells (MGCs) are resident macrophages in the brain and spinal cord, playing a crucial role in immune defense and transition between different states. Increased Nurr1 expression is associated with anti-neuroinflammatory responses. This study investigates the potential role of ferulic acid (FA) in facilitating microglia transition by inducing Nurr1 expression.
Article
Obstetrics & Gynecology
Saeideh Abdollahzadeh, Ahmad Riasi, Marziyeh Tavalaee, Farnoosh Jafarpour, M. H. Nasr-Esfahani
Summary: An imbalance between omega-6 and omega-3 fatty acids in sperm is correlated with lipid peroxidation and DNA damage in sperm, indicating a possible link to fertility potential. This study found that men with high DNA fragmentation had higher levels of saturated, monounsaturated, and omega-6 to omega-3 fatty acid ratios in sperm and seminal plasma compared to men with low DNA fragmentation. Additionally, there were positive associations between the omega-6 to omega-3 fatty acid ratio and sperm lipid peroxidation, DNA fragmentation, and residual histones in both sperm and seminal plasma.
REPRODUCTIVE SCIENCES
(2023)
Article
Multidisciplinary Sciences
M. Zanjirband, R. Hodayi, Z. Safaeinejad, M. H. Nasr-Esfahani, R. Ghaedi-Heydari
Summary: This study investigated the impact of the p53 pathway in enhancing apoptosis and abnormal function of granulosa cells. Results suggest that p53 target genes play a critical role in the pathogenesis of PCOS, specifically through the enhancement of apoptosis in granulosa cells.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Behrouz Aghajanloo, Fatemeh Ejeian, Francesca Frascella, Simone L. L. Marasso, Matteo Cocuzza, Alireza Fadaei Tehrani, Mohammad Hossein Nasr Esfahani, David W. W. Inglis
Summary: Deterministic lateral displacement (DLD) is a passive separation method for particle and cell sorting. It offers high size resolution and operates using capillary pressure, making it suitable for point-of-care devices. The method achieves high separation efficiency and is simple to use, making it a valuable technique in the field of microfluidic technologies.
