Editorial Material
Biology
J. Julie Kim
Summary: A new laboratory model helps to study the role of senescent cells in fostering a uterine environment that can support an embryo.
Review
Biochemistry & Molecular Biology
Manizha Shekibi, Sophea Heng, Guiying Nie
Summary: The development of endometrial receptivity is crucial for successful embryo implantation and pregnancy initiation. Recent studies have focused on the role of microRNAs (miRNAs) in the regulation of endometrial receptivity. This review provides an up-to-date knowledge of the miRNAs identified in humans and mice that may play a functional role in the establishment of receptivity. The findings suggest that miRNAs could be potential biomarkers and therapeutic targets for improving endometrial receptivity in human fertility treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Thomas M. Rawlings, Komal Makwana, Deborah M. Taylor, Matteo A. Mole, Katherine J. Fishwick, Maria Tryfonos, Joshua Odendaal, Amelia Hawkes, Magdalena Zernicka-Goetz, Geraldine M. Hartshorne, Jan J. Brosens, Emma S. Lucas
Summary: The study suggests that decidual senescence controls endometrial fate decisions at implantation and the presence of senescent decidual cells creates a dynamic implantation environment, enabling embryo expansion and attachment but leading to gradual disintegration of assembloids. Pharmacological inhibition of stress responses in pre-decidual cells accelerated decidualization and eliminated the emergence of senescent decidual cells, resulting in a strong, static decidual matrix. The research highlights how endometrial assembloids may facilitate the discovery of new treatments to prevent reproductive failure.
Article
Endocrinology & Metabolism
Federica Cariati, Consolata Carotenuto, Francesca Bagnulo, Daniela Pacella, Vincenzo Marrone, Rossella Paolillo, Maria Rosaria Catania, Raffaella Di Girolamo, Alessandro Conforti, Ida Strina, Carlo Alviggi
Summary: The study aimed to use a culturomics-based method to analyze the endometrial microbiota and correlate the results with ongoing pregnancy rates. It was found that detection of bacteria by culturomics from catheter tips used for embryo transfer could offer a reliable method to detect pathogen growth. The study highlights the importance of endometrial microbiota testing in improving diagnosis and treatment strategies in IVF patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chen Zhou, Xinyan Cheng, Fanming Meng, Yongzhong Wang, Wanyun Luo, Enqin Zheng, Gengyuan Cai, Zhenfang Wu, Zicong Li, Linjun Hong
Summary: This study analyzed the circRNA expression profile in the pig endometrium during the 12th day of gestation and found that circRNAs are abundantly expressed and play important regulatory roles in related genes. These findings provide insights into the molecular mechanisms contributing to pregnancy establishment and embryo loss in pigs, as well as the molecular pathway differences between breeds during the critical implantation period.
Article
Environmental Sciences
Yu-Ting Su, Jia-Shing Chen, Yi-Ru Tsai, Kuo-Chung Lan, Cheng-Chun Wu, Fu-Jen Huang
Summary: Mifepristone directly affects blastocyst viability and inhibits post-implantation embryo development in mice. The study suggests a potential risk of fetus fatality and developmental problems when pregnancies are continued after mifepristone treatment fails.
Review
Obstetrics & Gynecology
Awoniyi O. Awonuga, Olivia G. Camp, Husam M. Abu-Soud, Daniel A. Rappolee, Elizabeth E. Puscheck, Michael P. Diamond
Summary: The contributions of uterine endometrium and embryo in implantation success are still debated. Various measures, such as endometrial receptivity analysis (ERA), endometrial scratch (ES), endometrial microbiome, and intrauterine or intravenous measures, are recommended to improve implantation success. However, there is insufficient evidence to determine the relative importance of endometrium and embryo in implantation success and the utility of these measures, especially with euploid embryos. Evidence suggests that embryos can implant and develop on non-endometrium epithelium. The role of embryo euploidy in implantation success is currently underappreciated.
REPRODUCTIVE SCIENCES
(2023)
Article
Immunology
Rumiana Ganeva, Dimitar Parvanov, Nina Vidolova, Margarita Ruseva, Maria Handzhiyska, Katarina Arsov, Ivon Decheva, Dimitar Metodiev, Veselina Moskova-Doumanova, Georgi Stamenov
Summary: The objective of this study was to compare the quantities and ratios of immune cells in the endometrium during the mid-luteal phase between women with recurrent implantation failure (RIF) with successful and unsuccessful embryo implantation. Endometrial biopsies from 116 women undergoing Assisted Reproductive Technology (ART) were stained for different immune cells, and the quantities and ratios were compared based on the outcome of embryo implantation in the subsequent cycle. The results showed that certain ratios of immune cells were significantly different between the successful and unsuccessful implantation groups, and could be predictive factors for successful implantation in RIF patients.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Review
Obstetrics & Gynecology
Yubao Wei, Cuilian Zhang, Guoping Fan, Li Meng
Summary: Organoids have emerged as innovative models for biomedical research, providing insights into disease mechanisms and therapy development. Despite their limitations in fully mimicking in vivo tissues, organoids greatly benefit therapy development for reproductive clinics.
REPRODUCTIVE SCIENCES
(2021)
Article
Reproductive Biology
Daniel Veraguas-Davila, Maria Francisca Cordero, Soledad Saez, Darling Saez-Ruiz, Alejandro Gonzalez, Fernando Saravia, Fidel Ovidio Castro, Lleretny Rodriguez-Alvarez
Summary: The study evaluated the effects of zona pellucida removal on the in vitro and in vivo development of domestic cat embryos. Results showed that removal of the zona pellucida negatively impacted gene expression and prevented successful implantation of the embryos.
Article
Environmental Sciences
Chuanxin Zhang, Xueqi Dong, Xiaoyu Yin, Xinyi Yuan, Jiawei Wang, Jinzhu Song, Zhenzhen Hou, Cheng Li, Keliang Wu
Summary: 2-bromoacetamide (BAcAm), a new class of disinfection by-products (DBPs), is commonly found in drinking water worldwide. The high cytogenetic toxicity of BAcAm has raised concerns about its toxic effects on early embryonic development. In this study, we developed an optimized in vitro culture (IVC) system for peri-and early post-implantation mouse embryos and used it to assess the developmental toxicity of BAcAm. We observed that exposure to BAcAm led to a decrease in egg cylinder formation rate and aberrant lineage differentiation in a dose-dependent manner. Transcriptomic analysis further revealed that BAcAm exposure during early developmental stages affected the abundance of transcripts related to various biological processes, indicating its toxic effects on embryonic development. Therefore, this study provides a robust tool for studying the toxicology of chemicals during early embryonic development and highlights the developmental toxicity of BAcAm in mammals.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Endocrinology & Metabolism
Davoud Jafari-Gharabaghlou, Mostafa Vaghari-Tabari, Hajar Oghbaei, Laura Lotz, Reza Zarezadeh, Yeganeh Rastgar Rezaei, Mahnaz Ranjkesh, Mohammad Nouri, Amir Fattahi, Saba Nikanfar, Ralf Dittrich
Summary: This review highlights the role of various adipokines in embryo-maternal interactions, endometrial receptivity, and embryo implantation, as well as the underlying molecular mechanisms.
ENDOCRINE CONNECTIONS
(2021)
Article
Multidisciplinary Sciences
Harunobu Kagawa, Alok Javali, Heidar Heidari Khoei, Theresa Maria Sommer, Giovanni Sestini, Maria Novatchkova, Yvonne Scholte Op Reimer, Gael Castel, Alexandre Bruneau, Nina Maenhoudt, Jenna Lammers, Sophie Loubersac, Thomas Freour, Hugo Vankelecom, Laurent David, Nicolas Rivron
Summary: Human pluripotent stem cells cultured in specific conditions can efficiently form blastoids resembling blastocyst-stage analogues, providing a faithful model for investigating human implantation and development.
Article
Biochemistry & Molecular Biology
Chow-Seng Kong, Alexandra Almansa Ordonez, Sarah Turner, Tina Tremaine, Joanne Muter, Emma S. Lucas, Emma Salisbury, Rita Vassena, Gustavo Tiscornia, Ali A. Fouladi-Nashta, Geraldine Hartshorne, Jan J. Brosens, Paul J. Brighton
Summary: The study found that uterine natural killer cells play a crucial role in embryo biosensing and implantation process, highlighting their importance in selectively eliminating senescent decidual cells.
Article
Cell Biology
Qing Tian, Jia-Peng He, Can Zhu, Qiu-Yang Zhu, Yu-Gu Li, Ji-Long Liu
Summary: This study used single-cell RNA sequencing to investigate the molecular mechanism of embryo implantation in pigs. The researchers analyzed the interaction between embryo cells and maternal endometrial cells and identified global gene expression changes associated with embryo implantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Alice Newman-Sanders, Jackson C. Kirkman-Brown, Meurig T. Gallagher
Summary: This study revealed a significant lack of awareness among young adults in the UK regarding the potential impacts of gym lifestyles and supplementation on male infertility. Men were found to have a concerning lack of concern for their own fertility, with differences in awareness levels between men and women. It was also observed that men were more likely to consider making changes to their behavior if it had a long-term impact on their fertility compared to short-term effects.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Shachar Reuvenny, Michal Youngster, Almog Luz, Rohi Hourvitz, Ettie Maman, Micha Baum, Ariel Hourvitz
Summary: Using a machine-learning model to determine the optimal trigger days can improve the outcomes of antagonist protocol cycles in freeze-all or fresh transfer cycles, for all age groups. Implementing these models can more accurately predict the number of retrieved oocytes, optimizing physicians' decisions, balancing workloads, and creating more standardized yet patient-specific protocols.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Chao Chen, Qi Wen, Feng Deng, Rong Li, Ying Wang, Xiumei Zhen, Jing Hang
Summary: This study investigates the proteomic and phosphoproteomic differences in the endometrium of women with recurrent pregnancy loss (RPL) compared to healthy control women during different phases of the menstrual cycle. The results identify differentially expressed proteins and phosphorylated proteins, and highlight the insulin/cyclic nucleotide signalling pathway and AMPK/mTOR signalling pathway as major contributors to the abnormality of RPL endometrium. The findings provide insights into potential proteins associated with the pathogenesis of RPL and contribute to the identification of potential targets for RPL treatment.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Jaime Guerrero, Juan Carlos Castillo, Jorge Ten, Jose Antonio Ortiz, Belen Lledo, Domingo Orozco, Francisco Quereda, Andrea Bernabeu, Rafael Bernabeu
Summary: The study found no significant differences in clinical outcomes between using oocytes obtained from random-start protocols and those from conventional ovarian stimulation in oocyte donation treatments. Luteal-phase stimulation required longer stimulation and higher FSH consumption.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)